Tissue Function-Reactive Oxygen Cross-talk in Oral Region : Its Pathopharmacological Analysis

The physiological importance of The oxygen free radical system was revealed by The发现activated neutrophils produce the superoxide anion radical (o_2 ^<·->). the o_2 ^<·-> cascade(producing h_2_2 and hydroxylradical，（HO） established in basic chemistry stimulated investigators of reactive oxygen species in numerouspathophysiological processes. The first target of reactive oxygen species，generated in several pathological processes, is the vascular system. in this研究项目，we wished to投资the mechanisms of the effect of reactive oxygen species on vascular smooth1. the HO radical directly produces vasoconstriction in the dental pulpin dogs. This could be explained by the destruction of basally released endothelium-derivedrelaxing factor, possibly nitric oxide，and/or endothelial dysfunction. The selective effect of this radical on endothelium-dependentrelaxation of pulpal vessels is supported by the finding that the increase…More in blood flow caused by a direct-acting, endothelium-independent dilatornitroglycerin, but not by acetylcholine，was unaffected by the exposure to the HO radical.2.我们发现canine lingual artery are canine lingual artery areinnervated by calcitonin gene-related peptide (CGRP)-containing vasodilator nerves. The HO radical，rather than h_2_2 to be the active agent in disturbance of CGRP-mediated neurogenic relaxiation，这可以被支持HO可以deplete endogenous CGRP localized prejunctionally and alsodamage CGRP-induced relaxation of canine lingual artery that is caused by activation ofat -sensitive K + channels at postjunctional sites.3. We evaluated the effects of reactive oxygenspecies on contractions induced by caffeine，Ins (1,4,5) p_3 and noradrenaline (NA) in Staphylococcal alpha- toxin_permeabilized rabbit mesentericartery. The pathway of Ca <2+> release from sarcoplasmic reticulum (SR) dependent on Ins （1,4,5）p_3 is insensitive too_2 . Instead，caffeine-induced Ca^<2+> release mechanism may be susceptible to o_2 ^<·->,and h_2_2，rather than o_2 and ho_2可以在nas -induced contraction中主动地参与其中。the view that the attenuation by h_2_2 of the NA-induced contraction may be linked to therecepter -associated pathway of Ins (1,4,5) p_3 formation.We also assessed the effect of singletoxygen (^ o_2) on vascular reactivity of rabbit mesenteric artery ring preparations. ^ o_2 generatedfrom photolysis of rose bengal is the potent destracive oxygen.****. Endothelium-dependentrelaxation is quite vulnerable to 1_2 is depresses NA-induced contraction possibly viaalpha-adrenoceptor dysfunction.If the sequence of events during pathophysiological processes such asperiodontitis in the vascular wall as well （especially in endothelial cells）这通常会导致区域血flow，这通常会导致tissue injury. Less

The physiological importance of the oxygen free radical system was revealed by the discovery that activated neutrophils produce the superoxide anion radical (O_2^<・->). The O_2^<・-> cascade (producing H_2O_2 and hydroxylradical, HO・) established in basic chemistry stimulated investigators of reactive oxygen species in numerous pathophysiological processes. The first target of reactive oxygen species, generated in several pathological processes, is the vascular system. In this research project, we wished to investigate the mechanisms of the effect of reactive oxygen species on vascular smooth muscle at the cellular level.1. The HO・radical directly produces vasoconstriction in the dental pulp in dogs. This could be explained by the destruction of basally released endothelium-derived relaxing factor, possibly nitric oxide, and/or endothelial dysfunction. The selective effect of this radical on endothelium-dependent relaxation of pulpal vessels is supported by the finding that the increase … More in blood flow caused by a direct-acting, endothelium-independent dilator, nitroglycerin, but not by acetylcholine, was unaffected by the exposure to the HO・radical.2. We found that canine lingual artery are innervated by calcitonin gene-related peptide (CGRP)-containing vasodilator nerves. The HO・radical, rather than H_2O_2, to be the active agent in disturbance of CGRP-mediated neurogenic relaxiation, and it can be suggested that HO・can deplete endogenous CGRP localized prejunctionally and also damage CGRP-induced relaxation of canine lingual artery that is caused by activation of ATP-sensitive K^+ channels at postjunctional sites.3. We evaluated the effects of reactive oxygen species on contractions induced by caffeine, Ins (1,4,5) P_3 and noradrenaline (NA) in Staphylococcal alpha-toxin-permeabilized rabbit mesenteric artery. The pathway of Ca^<2+> release from sarcoplasmic reticulum (SR) dependent on Ins (1,4,5) P_3 is insensitive to O_2^<・->. Instead, caffeine-induced Ca^<2+> release mechanism may be susceptible to O_2^<・->, and H_2O_2, rather than O_2^<・-> and HO・, may be the active agent in the NA-induced contraction. The results obtained are also consistent with the view that the attenuation by H_2O_2 of the NA-induced contraction may be linked to the receptor-associated pathway of Ins (1,4,5) P_3 formation.We also assessed the effect of singlet oxygen (^1O_2) on vascular reactivity of rabbit mesenteric artery ring preparations. ^1O_2 generated from photolysis of rose bengal is the potent destracive oxygen.****. Endothelium-dependent relaxation is quite vulnerable to ^1O_2 is depresses NA-induced contraction possibly via alpha-adrenoceptor dysfunction.If the sequence of events during pathophysiological processes such as periodontitis in the vascular wall as well (especially in endothelial cells), it may lead to disturbances of regional blood flow, which may aggravate the tissue injury. Less