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DEVELOPMENT OF 2D LINEAR AND CIRCULAR DICHROMETER AND ITS APPLLICATION FOR BIOLOGICAL MOLECULAR SYSTEMS

二维线性和圆形二色计的研制及其在生物分子系统中的应用

基本信息

批准号：
06555257
负责人：
NOZAWA Tsunenori
金额：
$ 11.26万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The goal of this research project consist of (1) development of multipurpose spectrophotometer enable to measure simultaneous electric absorption, linear, circular and mangetic circular dichroism on the same sample with the same observation light, (2) transformation of the signals into two dimensional correlation, and (3) application of the spectrophotometer to biological molecular systems. It has been recognized that we need multiple information to analyze the structure and function of the biologically interesting system. However untill recently, it is necessary to take different spectroscopic data on different devices, and sometimes we must use different type of sample to get different spectoscopic data. Therefore the multiple spectroscopic photometer is anticipated to give the many information simultaneously to enable automatic understanding and yield user friendly data. In this project we tried to construct a spectrometer to enable us to measure simultaneously the electric absorption, linear, circular and magnetic circular dichroism data. The two dimensional analysis of the data need to collect a series of spectra under dynamic perturbation, and to calculate the correlation spectra by Fourier-transformation of the dynamic spectra. We have succeeded to get the simultaneous correlation and anti simultaneous spectra for the oxidation reduction process of the cytochrome c in the photosynthetic reaction center from a purple sulfur bacterium Chromatium tepieum. We are still in the stage of the development, but the fundamental construction and transformation into two dimensional spectra have been accomplished.

本课题的研究目标包括：(1)研制能够在同一观察光下同时测量同一样品的电吸收、线性、圆形和磁性圆二色性的多用途分光光度计；(2)将信号转化为二维相关；(3)将分光光度计应用于生物分子体系。人们已经认识到，我们需要多种信息来分析生物感兴趣系统的结构和功能。然而，到目前为止，需要在不同的设备上采集不同的光谱数据，有时还必须使用不同类型的样品来获得不同的光谱数据。因此，多光谱光度计有望同时提供多种信息，以实现自动理解并产生用户友好的数据。在这个项目中，我们试图构建一个光谱仪，使我们能够同时测量电吸收，线性，圆形和磁性圆二色性数据。对数据进行二维分析需要采集动态摄动下的一系列光谱，并对动态光谱进行傅里叶变换计算相关光谱。我们成功地获得了紫硫菌铬酸盐光合反应中心细胞色素c氧化还原过程的同时相关光谱和反同时光谱。目前尚处于发展阶段，但已基本完成了二维光谱的构建和转换。