MECHANISM AND STRUCTURE OF PHOTOSYNTHETIC REACTION AND APPRATUS FOR APPLICATION OF ITS MECHANISM TO ENERGY PRODUCING BIOMEMBRANE

光合反应的机理和结构及其应用于产能生物膜的装置

• 批准号: 07455437
• 负责人: NOZAWA Tsunenori
• 金额: $ 1.15万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07455437/
• 关键词: PHOTOSYNTHETIC MEMBRANE; REACTION CENTER COMPLEX; MEMBRANE ANTENNA; CHLOROSOME ANTENNA; SOLID STATE NMR; X-RAY DIFFRACTION SPECTROSCOPY; ATP SYNTHESIS; P-31 NMR; 膜内アンテナ; X線分光法; ATP合成反応; ^<31>P NMR; 光合成反応; エネルギー産生生体膜; 光ATP合成活性; 光合成膜顆粒; ^<13

Bio-membranes play key roles in many vital processes in life, especially energy production, and information transfer. In this project we have investigate the energy conversion processes in photosynthesis with an intention to apply its mechanism for construction of semi-synthetic artificial solar energy conversion. Photosynthetic living convert light energy to chemical energy by (1) capturing light energy and conversing it to excitation energy in antenna complex, (2) changing the excitation energy to electro-chemical energy in reaction center and electron transfer complexes, and (3) making the chemical energy ATP (adenosine tri-phosphate) in ATP ase. We have clarified the light capturing mechanism of antenna both on chlorosome and membrane antenna. The primary structures of the reaction center for a novel thermo-philic photosynthetic bacteria have been determined for the first time. The fine structures which modulate the electron transfer ability of reaction center have been clarified by electric and spectroscopic methods. Organization of pigments have been elucidated by magnetic circular dichroism methods for the antenna and photosynthetic membranes. Photosynthetic membrane vehicles (chromatophore) which contain antenna, reaction exenter, electron transfer protein and ATP ase have been studied with respect to its size distribution and photo-phosphorylation activity.

生物膜在生命的许多重要过程中起着重要的作用，尤其是能量的产生和信息的传递。在本项目中，我们研究了光合作用中的能量转换过程，意图将其机制应用于半合成人工太阳能转换的构建。光合生物将光能转化为化学能的途径是：(1)在天线络合物中捕获光能转化为激发能，(2)在反应中心和电子转移络合物中将激发能转化为电化学能，(3)在ATP酶中产生化学能ATP （adenosine triphosphate）。从叶绿体天线和膜天线两方面阐明了天线的光捕获机理。首次确定了一种新型亲热光合细菌反应中心的初级结构。通过电学和光谱学的方法，阐明了影响反应中心电子转移能力的精细结构。用磁性圆二色性方法对天线膜和光合膜的色素组织进行了研究。对含有天线、反应中心、电子转移蛋白和ATP酶的光合膜载体（染色质）的大小分布和光磷酸化活性进行了研究。

项目成果

Zheng-Yu Wang et al.: ""Size distributi on and photophosphorylation of chromatophores from Rhodospirillum rubrum"" Photosynthesis Research. (in press).

王正宇等人：“红色红螺菌色素细胞的大小分布和光磷酸化”光合作用研究。

Anabella Ivancich et al.,: "Hydrogen-bond interactions of the primary donor of the photosynthetic purple sulfur bacterium Chromatium tepidum" Biochemistry. 35. 10529-10538 (1996)

Anabella Ivancich 等人：“光合紫硫细菌 Chromatium tepidum 的主要供体的氢键相互作用”生物化学。

John T.M.Kennis et al.: ""Energy transfer and exciton coupling in isolated B800-850 complexes of the photosynthetic purple sulfur bacterium Chromatium tepidum. The effect of structural symmetry on bacteriochlorophyll excited states"" J.Phys.Chem.100. 2438

John T.M.Kennis 等人：“光合紫色硫细菌 Chromatium tepidum 的分离 B800-850 复合物中的能量转移和激子耦合。

野澤庸則(分担執筆): "新タンパク質応用工学(第5章第2節,第11章第11節)" フジ・テクノシステム(監修籏野昌弘), 19 (1996)

Yoshinori Nozawa（撰稿人）：“新蛋白质应用工程（第 5 章第 2 节、第 11 章第 11 节）”Fuji Techno System（加野正宏监督），19（1996 年）

Insan Fathir et al.,: "The genes coding for the L,M and cytochrome subunits of the photosynthetic reaction center from the thermophilic purple sulfur bacterium Chromatium tepidum" Photosynthesis Research. 51. 71-82 (1997)

Insan Fathir 等人：“编码嗜热紫硫细菌 Chromatium tepidum 光合反应中心 L、M 和细胞色素亚基的基因”光合作用研究。