Structural Elucidation of Photosynthetic antenna and Its Application for Light Harvesting System.

光合天线的结构解析及其在光捕获系统中的应用。

• 批准号: 03650638
• 负责人: NOZAWA Tsunenori
• 金额: $ 1.15万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03650638/
• 关键词: Thermostability; Photosynthetic Bacteria; Photosynthetic Antenna; CP; MAS^<13>C NMR; Chlorophyll; Excitation Energy Transfer; FTIR; Time Resolved Fluorescence; NMR; 光合成色素; ラマン; IR; 会合構造; CP; MAS NMR

We have succeeded in preparing photosynthetic antennas from photosynthetic membranes of Chloroflexus aurantiacus and Chlorobium tepidum. The isolated antenna chlorosomes were studied by a solid state high resolution (cross polarization / magic angle spinning (CP/MAS)) ^<13>CNMR. We have clarified the cooridation states of the bacteriochlorophyll (BChl) c's, the interactions between BChl c's and the interactions of BChl c's with surrounding amino acid residues in native states and in situ. We have purified various BChl c and d derivatives by extraction with organic solvent and HPLC. The structures of the pigments were determined with one and two dimensional ^1H and ^<13>C NMR and FTIR. Excitation energy transfers in the antenna systems and also between the antennas and reaction centers were investigated with a pico-second time resolved fluorescence spectrometry. The results gave an evidence to elucidate the structures and mechanisms for the antenna systems to collect and utilize the light energy. The model antenna systems which resembled the native chlorosome antenna have been prepared for the first time from the purified BChl c and d derivatives with clarified structures. The organization of the synthesized model antenna were investigated with CP/MAS^<13>C NMR and FTIR. These investigations offered a guide line to construct a model antenna system and to utilize light energy efficiently.

我们成功地从橙黄绿弯藻和绿球藻的光合膜中制备了光合天线。用固体高分辨（交叉极化/魔角自旋（CP/MAS））~（13）CNMR研究了分离的天线绿小体<13>。我们已经澄清了细菌叶绿素（BChl）c的配位状态，BChl c之间的相互作用和BChl c与周围的氨基酸残基在天然状态和原位的相互作用。我们已经纯化了各种BChl的c和d衍生物的有机溶剂萃取和HPLC。用一维和二维核磁共振氢谱、碳谱和傅里叶变换红外光谱确定了颜料的结构<13>。用皮秒时间分辨荧光光谱研究了天线系统中以及天线与反应中心之间的激发能转移。研究结果为阐明天线系统收集和利用光能的结构和机制提供了依据。从纯化的BChl c和d衍生物中首次制备了类似于天然叶绿体天线的模型天线系统。用CP/MAS ~ 1C NMR和FTIR研究了合成的模型天线的结构<13>。这些研究为构造模型天线系统和有效利用光能提供了指导。

项目成果

T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita and H.Konami: "Progress in Photosynthesis Research C/MAS ^<13>CNMR Studies on Antenna Structures in Chlorosomes in Green Bacteria" Kluwer Academic Publishers, 4 (1993)

T.Nozawa、K.Ohtomo、M.Suzuki、Y.Morishita 和 H.Konami：“光合作用研究进展 C/MAS ^<13>CNMR 研究绿色细菌叶体天线结构”Kluwer 学术出版社，4（1993 年）

T.NOZAWA,K.OHTOMO,M.SUZUKI,Y.MORISHITA and Michael T.Madigan: "Structures and Organization of Bacteriochlorophyll c′s in Chlorosomes from a New Thermophilic Chlorobium tepidum" Bull.Chem.Soc.Jpn.66. 1-7 (1993)

T.NOZAWA、K.OHTOMO、M.SUZUKI、Y.MORISHITA 和 Michael T.Madigan：“来自新的嗜热绿菌叶绿素 cs 的结构和组织”Bull.Chem.Soc.Jpn.66。 -7 (1993)

T.Nozawa,K.Ohtomo,M.Suzuki,Y.Morishita,H.Konami and Michael T.Madigan: "Structures of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Chem.Lett.1763-1766 (1991)

T.Nozawa、K.Ohtomo、M.Suzuki、Y.Morishita、H.Konami 和 Michael T.Madigan：“来自新型嗜热细菌 Chlorobium tepidum 的叶绿素 c 的结构” Chem.Lett.1763-1766 (1991)

T.NOZAWA,K.OHTOMO,N.TAKESHITA,Y.MORISHITA,M.OHSAWA and Michael T.Madigan: "Substituent Effects on the Aggregation of Bacterochlorophyll d Homologues Purified from Chlorobium limicola" Bull.Chem.Soc.Jpn.65. 3493-3494 (1992)

T.NOZAWA、K.OHTOMO、N.TAKESHITA、Y.MORISHITA、M.OHSAWA 和 Michael T.Madigan：“取代基对从 Chlorobium limicola 纯化的细菌叶绿素 d 同系物聚集的影响”Bull.Chem.Soc.Jpn.65。

T.Nozawa, K.Ohtomo, M.Suzuki, Y.Morishita, H.Konami and Michael T.Madigan: "Structures of Bacteriochlorophyll c's in Chlorosomes from a New Thermophilic Bacterium Chlorobium tepidum" Chem. Lett.1763-1766 (1991)

T.Nozawa、K.Ohtomo、M.Suzuki、Y.Morishita、H.Konami 和 Michael T.Madigan：“来自新型嗜热细菌 Chlorobium tepidum 的叶绿素 c 的结构” Chem。