Breeding of the Soybean Lines with High Quality Edible Oil Utilizing a Lipoxigenase-Lacking Trait in Seed

利用种子缺乏脂氧合酶的特性选育优质食用油大豆品系

• 批准号: 06556001
• 负责人: KAIZUMA Norihiko
• 金额: $ 1.73万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06556001/
• 关键词: Artificial Mutation Induction; Soybean Seed Proteins; Lipoxigenase; Edible Oil; 人為突然変異; プロテイン・ボディ; スフェロゾーム

The ultimate objective of this study is to develope completely lipoxigenase-lacking soybean lines in seeds which are characterized with oil deterioration-resistance by effect of no or less oxidation of non-suturated fatty acids.Some promising soybean F3 lines were selected from the hybridization between Kyushu 11 (lypoxigenase-lacking) and high oil Jilin 11/Williams in the Agricutural Research Center. However, oil analyzes and oil-deterioration tests are now in progress.On the other hand, the soybean lines accumulating some seed protein-subunit lacking variant genes (7S and 11S globulin-subunits) through hybridizations were developed in Iwate University for future hybridization programs with lipoxigenade-lacking lines. SDS-PAGE analyzes using about 3,500 F2 seeds were carried out for genetic analyzes of the protein-subunit lacking traits. A part of them were used for oil and protein contents analyzes and very interesting results were obtained.Electron microscopic observations of the protein bodies and spherosomes in the 7S and/or 11S globulin subunit-lacking variants brought some interesting results.

本研究的最终目的是通过不氧化或少氧化非缝合脂肪酸，在种子中培育完全缺乏脂肪氧合酶的大豆品系。从九州11号(缺乏脂氧合酶)与高油吉林11/Williams杂交中筛选出一批有前景的大豆F3系。另一方面，岩手县大学通过杂交积累了一些缺乏变异基因的种子蛋白亚基(7S和11S球蛋白亚基)的大豆品系，用于未来与脂氧素缺失品系的杂交计划。对大约3500粒F2代种子进行了蛋白质亚基缺失性状的遗传分析。其中一部分用于油脂和蛋白质含量的分析，得到了非常有趣的结果。对7S和/或11S球蛋白亚基缺失变体的蛋白质小体和球体进行了电子显微镜观察，得到了一些有趣的结果。

项目成果

Kitamura,K.: "Breeding trials for improving the food processing quality of soybeans" Trend in Food Sci. & Tech.41. 64-67 (1993)

Kitamura,K.：“提高大豆食品加工质量的育种试验”食品科学趋势。

Phan, T.H., et al.: "Specific inheritance of a mutant gene controlling alfa, beta subunits-null beta conglycinin in soybean (Glycine max (L.) Merrill) and observation of chloroplast ultrastructure of the mutant" Breeding Science. 46. 53-59 (1996)

Phan, T.H. 等人：“控制大豆 (Glycine max (L.) Merrill) 中α、β 亚基无效 β 伴大豆球蛋白的突变基因的特异性遗传以及突变体叶绿体超微结构的观察”《育种科学》。

喜多村啓介: "Breeding trials for improving the food processing quality of soybeans" Trends in Food Sci.& Tech.41. 64-67 (1993)

Keisuke Kitamura：“提高大豆食品加工质量的育种试验”Trends in Food Sci.& Tech.41 (1993)。

Inamori, Y., et al.: "Genetic analysis of subunits of seed storage proteins in the hybrids between a line lacking alfa and alfa-prime subunits of 7S globulins and lines lacking all the 11S globulins subunits" Breeding Science. 46 Suppl.2. 101-101 (1996)

Inamori, Y., 等人：“缺乏 7S 球蛋白的 alfa 和 alfa-prime 亚基的品系与缺乏所有 11S 球蛋白亚基的品系之间的杂种中种子储存蛋白亚基的遗传分析”《育种科学》。

Kitamura,K.: "Genetic improvement of nutritional and food processing quality in soybean" JARQ. 29. 1-8 (1995)

Kitamura,K.：“大豆营养和食品加工质量的遗传改良”JARQ。