Comparative Study on Species-Specific Seed Constituents Differenciating between Wild and Cultivated Soybeans

野生大豆和栽培大豆种特异性种子成分差异的比较研究

• 批准号: 06660004
• 负责人: KAIZUMA Norihiko
• 金额: $ 1.15万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06660004/
• 关键词: Soybeans; Wild Soybeans; Trypsin-Inhibitor; Seed Proteins; Ecotype Differenciation; Gene Resources; 種子タンパク質

The chemical seed constituents differentiating between cultivated and wild soybeans were not detected. However, partitional ratios of the constituents among leaves, stems and seeds seemed to differ specifically between the two species.Estimation of the north and south limits in growing area of wild soybean in Japan was determined as in the southern Hokkaido and Kyushu by field investigations.Principal Component Analysis using the 30 morphological traits revealed that at least the two ecotypes exisi in Japan, Southern and Northern.Some new electrophoretic polymorphic types of Kunitz trypsin inhibitor in seed were clearly detected. The DNA sequence of a new type named Tia-s was analyzed. As a result, an insertion and a substitution of the DNA bases of the gene and their respective Amino Acid changes were found.

未检测到区别栽培大豆和野生大豆的化学成分。通过对30个形态性状的主成分分析表明，日本野生大豆的南北界分别为北海道南部和九州，其中至少有两个生态型的野大豆，Southern和北方两个区域。在种子中清晰地检测到Kunitz胰蛋白酶抑制剂的一些新的电泳多态类型。分析了一个新的类型Tia-s的DNA序列。结果，发现了该基因的DNA碱基的插入和取代以及它们各自的氨基酸变化。

项目成果

王克晶ら: "ダイズKunitz型トリプシン・インヒビターTia-s変異体の遺伝分析と塩基配列決定" 育種学雑誌. 46別2. 244 (1996)

Kejing Wang 等人：“大豆 Kunitz 型胰蛋白酶抑制剂 Tia-s 突变体的遗传分析和核苷酸测序”《育种杂志》46 第 2 部分。244 (1996)

Wang, J.K., et al.: "Detection of two new variants of soybean Kunitz trypsin inhibitor through electrophoresis" Breeding Science. 46. 39-44 (1996)

Wang, J.K. 等人：“通过电泳检测大豆 Kunitz 胰蛋白酶抑制剂的两种新变体”《育种科学》。

王 克晶: "ダイズトリプシン・インヒビターの新しい電気泳動的多型" 育種学雑誌. 43(別2). 177〜177 (1993)

王科静：“大豆胰蛋白酶抑制剂的新电泳多态性”，育种杂志43（第2部分）（1993）。

Wang,J.K.,et al.: "The mode of inheritance and the DNA sequence analysis for a new soybean Kunitz type trypsin inhibitor vaariant, Tia-s" Breeding Science. 46,Suppl.2,. 89-89 (1996)

Wang,J.K.,et al.：“新型大豆 Kunitz 型胰蛋白酶抑制剂变体 Tia-s 的遗传模式和 DNA 序列分析”育种科学。

Wang,J.K.,et al.: "Detection of two new variants of soybean Kunitz trypsin inhibitor through electrophoresis" Breeding Science. 46. 39-44 (1996)

Wang,J.K.,et al.：“通过电泳检测大豆 Kunitz 胰蛋白酶抑制剂的两种新变体”《育种科学》。