Comparative Study on Species-Specific Seed Constituents Differenciating between Wild and Cultivated Soybeans

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The chemical seed constituents differentiating between cultivated and wild soybeans were not detected. However, partitional ratios of the constituents among leaves, stems and seeds seemed to differ specifically between the two species.Estimation of the north and south limits in growing area of wild soybean in Japan was determined as in the southern Hokkaido and Kyushu by field investigations.Principal Component Analysis using the 30 morphological traits revealed that at least the two ecotypes exisi in Japan, Southern and Northern.Some new electrophoretic polymorphic types of Kunitz trypsin inhibitor in seed were clearly detected. The DNA sequence of a new type named Tia-s was analyzed. As a result, an insertion and a substitution of the DNA bases of the gene and their respective Amino Acid changes were found.