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The development of a new diagnostic method for uterine endometrial cancer, using monoclonal antibody for glicolipids, and its application for mass screening

使用糖脂单克隆抗体开发子宫内膜癌新诊断方法及其在大规模筛查中的应用

基本信息

批准号：
06557088
负责人：
NOZAWA Shiro
金额：
$ 4.1万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557088/
关键词：
endometrial cancer monoclonal antibody MSN-1 enzyme immunoassay

项目摘要

We measured the levels of the "MSN-1" antigen (mainly the Lewis^b sugar chain) in endometrial cell samples using an enzyme immunoassay (EIA) with the anti-uterine cancer monoclonal antibody MSN-1. We developed a new diagnostic EmC-EIA (Endometrial Cell Enzyme Immunoassay) method for endometrial cancer which enabled distinction between normal endometrial cells and cancer cells according to quantitative differences in the level of MSN-1 antigen.The original EmC-EIA method was also improved. Since long term storage of samples is possible and the procedure is simple, this EmC sandwich EIA method could easily be made into a kit. Its clinical applicability was confirmed using many clinical samples. The EmC sandwich EIA method involves solubilization with surfactant of the residual cells adhering to the sample collection device after endometrial cytodiagnosis. The primary antibodies for actin (protein content) and MSN-1 are bound to a microtiter plate. Subsequently, the amount of MSN-1 antige … More n per unit protein in the cell sample is measured by a sandwich EIA using enzme-labeled MSN-1 and antiactin antibodies. We investigated 48 samples of normal endometrium, 11 of endometrial hyperplasia, and 45 of endometrial cancer. The positive rate (when the mean +2 SD for normal endometrium was taken as the cut-off value) was 4.2% for normal endometrium, 45.5% for endometrial hyperplasia, and 66.7% for endometrial cancer. The positive rate was 76% for well-differentiated endometrial adenocarcinoma, a particularly high value. In a study of 35 patients with uterine cancer who underwent both cytodiagnosis and the EmC sandwich EIA,the positive rate was 63.3% for cytodiagnosis and 63.3% for the EmC sandwich EIA.However, when the two methods were combined, the positive rate rase 83.3%.In conclusion, the EmC sandwich EIA was found to be useful as a auxiliary procedure to cytodiagnosis in patients with uterine cancer, especially those with well-differentiated adenocarcinoma which is often hard to evaluate by cytodiagnosis. Since this assay has various features such as application as a kit, it could also be used for screenig. Less

我们使用抗子宫癌单克隆抗体MSN-1的酶免疫测定法（EIA）测定了子宫内膜细胞样本中“MSN-1”抗原（主要是刘易斯^B糖链）的水平。我们建立了一种新的诊断子宫内膜癌的EMC-EIA（Endometrial Cell Enzyme Immunoassay）方法，该方法根据MSN-1抗原水平的定量差异来区分正常子宫内膜细胞和癌细胞，并对原有EMC-EIA方法进行了改进。由于该方法可长期保存样品，且操作简单，可方便地制成试剂盒。使用许多临床样本证实了其临床适用性。EmC夹心EIA方法包括在子宫内膜细胞诊断后用表面活性剂溶解粘附在样品收集装置上的残留细胞。肌动蛋白（蛋白质含量）和MSN-1的一抗与微量滴定板结合。随后，MSN-1 antige ...更多信息使用酶标记的MSN-1和抗肌动蛋白抗体通过夹心EIA测量细胞样品中每单位蛋白的n。我们研究了48例正常子宫内膜，11例子宫内膜增生症和45例子宫内膜癌。正常子宫内膜、子宫内膜增生症和子宫内膜癌的阳性率分别为4.2%、45.5%和66.7%。高分化子宫内膜腺癌的阳性率为76%，特别高。对35例子宫癌患者同时进行细胞学诊断和EmC夹心EIA的研究表明，细胞学诊断的阳性率为63.3%，EmC夹心EIA的阳性率为63.3%，两者联合应用的阳性率为83.3%。因此，EmC夹心EIA可作为子宫癌细胞学诊断的一种辅助方法。尤其是那些具有通常难以通过细胞诊断评估的高分化腺癌的患者。由于该检测方法具有多种特点，如作为试剂盒应用，因此也可用于筛选。少