Glycolipids analysis in female genital organs originated from Muller's duct : relationship to the extracellular matrix

本研究的目的是分析子宫内膜和输卵管内的糖脂，通过分析糖脂与细胞外基质的关系，从糖结合物的角度阐明子宫内膜癌的受精和受精机制，以及子宫内膜癌的增殖和转移机制，建立一种新的子宫内膜癌诊断技术。我们得到了以下的发现。子宫内膜及输卵管内糖脂及细胞外基质分析：正常子宫内膜增生期仅检出少量硫脂，中性糖脂神经酰胺未见羟基化。另一方面，在分泌期，神经酰胺的羟基化作用显著增加。在整个性周期中，输卵管中都有硫醚的表达。与自脂质一样，层粘连蛋白明显与硫脂质具有高亲和力，当细胞由增殖期转变为分泌期时，层粘连蛋白的表达开始增加。高危子宫内膜增生筛查基础资料的收集：据报道，我们实验室的单克隆抗体MSN-1主要识别细胞表面的糖脂，并与90%以上的子宫内膜癌标本发生反应。观察期间，部分子宫内膜增生发展为子宫内膜癌。应用免疫组织染色法测定发展为子宫内膜癌患者的MSN-1反应性。最终发展为子宫内膜癌的子宫内膜增生组的阳性率高于对照组；囊性增生75%，腺瘤性增生78%，非典型增生100%。这些结果提示，分析MSN-1识别抗原的表达可能有助于筛查高危子宫内膜增生。子宫内膜癌诊断新技术的建立：我们建立了一种新的MSN-1酶免疫分析法（EmC-EIA法），并对其作为子宫内膜癌诊断新技术的可行性进行了研究。各组阳性率计算如下：子宫内膜正常组5.7%，子宫内膜增生组20.0%，子宫内膜癌组77.3%。我们的新方法与常规临床检查的细胞诊断相结合，提高了高分化子宫体癌组的诊断正确率，阳性率高达89.5%。因此，电磁-环评法可作为子宫内膜癌的辅助诊断技术。少

The purposes of the present study are to analyze glycolipid in the endometrium and fallopian tube, to clarify not only the mechanism of fecundation and nidation but also the mechanism of the proliferation and metastasis of uterine endometrial carcinoma from the viewpoint of glycoconjugates by analyzing the relation between glycolipid and extracellular matrix and to establish a new diagnostic technique for uterine endometrial carcinoma. We obtained the following findings.1.Analysis of glycolipid and extracellular matrix in the endometrium and fallopian tube :In the normal endometrium, a small amount of sulfatide and no hydroxylation of neutral glycolipid ceramide were detected in the proliferative phase. On the other hand, in the secretory phase, sulfatide increased remarkably and hydroxylation of ceramide was observed. Sulfatide was expressed in the fallopian tube throughout the sexual cycle. As in the case of selfatide, laminin, which apparently bound sulfatide with high affinity, sta … More rted to increase when the phase changed from proliferative phase to secretory phase.2.Collection of basic data for the screening of high resk endometrial hyperplasia :It has benn reported that MSN-1, a monoclonal antibody in our laboratory, mainly recognizes glycolipid on the cell surface and reacts with over 90% of uterine endometrial carcinoma specimens. During the observation period, endometrial hyperplasia developed into endometrial carcinoma in some cases. MSN-1 reactivity was determined by immunohistological staining in the cases developed into endometrial carcinoma. The positive rates calculated in the group of endometrial hyperplasia eventually developed into uterine endometrial carcinoma were higher than the positive rates calculated in the control group ; 75% in cystic hyperplasia cases, 78% in adenomatous hyperplasia cases, 100% in atypical proliferation cases. These results suggested the possibility that the analysis of the expression of the antigen recognized by MSN-1 was useful for the screening of high risk endometrial hyperplasia.3.Development of a new diagnostic technique for uterine endometrial carcinoma :We developed a new method of enzyme immunoassay with MSN-1(EmC-EIA method) and studied its usefulness as a new diagnostic technique for uterine endometrial carcinoma. The positive rate calculated in each group was as follows : 5.7% in the group woth normal uterine endometrium, 20.0% in the group with endometrial hyperplasia, 77.3% in the group of uterine endometrial carcinoma.The combination of our new method and cytodiagnosis performed as a routine clinical examination secured more correct diagnosis and increaed the positive rate up to 89.5% in the group with highly differentiated uterine body carcinoma. Therefore the EmC-EIA method was considered to be usuful as an ancillary diagnostic technique for uterine endometrial carcinoma. Less