Glycolipids analysis in female genital organs originated from Muller's duct : relationship to the extracellular matrix

来自米勒管的女性生殖器官中的糖脂分析：与细胞外基质的关系

• 批准号: 04454424
• 负责人: NOZAWA Shiro
• 金额: $ 4.74万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454424/
• 关键词: endometrium; fallopian tube; glycolipid; extracellular matrix; sulfatide; MSN-1; endometrial carcinoma enzyme immunoassay; 酵素免疫測定法

The purposes of the present study are to analyze glycolipid in the endometrium and fallopian tube, to clarify not only the mechanism of fecundation and nidation but also the mechanism of the proliferation and metastasis of uterine endometrial carcinoma from the viewpoint of glycoconjugates by analyzing the relation between glycolipid and extracellular matrix and to establish a new diagnostic technique for uterine endometrial carcinoma. We obtained the following findings.1.Analysis of glycolipid and extracellular matrix in the endometrium and fallopian tube :In the normal endometrium, a small amount of sulfatide and no hydroxylation of neutral glycolipid ceramide were detected in the proliferative phase. On the other hand, in the secretory phase, sulfatide increased remarkably and hydroxylation of ceramide was observed. Sulfatide was expressed in the fallopian tube throughout the sexual cycle. As in the case of selfatide, laminin, which apparently bound sulfatide with high affinity, sta … More rted to increase when the phase changed from proliferative phase to secretory phase.2.Collection of basic data for the screening of high resk endometrial hyperplasia :It has benn reported that MSN-1, a monoclonal antibody in our laboratory, mainly recognizes glycolipid on the cell surface and reacts with over 90% of uterine endometrial carcinoma specimens. During the observation period, endometrial hyperplasia developed into endometrial carcinoma in some cases. MSN-1 reactivity was determined by immunohistological staining in the cases developed into endometrial carcinoma. The positive rates calculated in the group of endometrial hyperplasia eventually developed into uterine endometrial carcinoma were higher than the positive rates calculated in the control group ; 75% in cystic hyperplasia cases, 78% in adenomatous hyperplasia cases, 100% in atypical proliferation cases. These results suggested the possibility that the analysis of the expression of the antigen recognized by MSN-1 was useful for the screening of high risk endometrial hyperplasia.3.Development of a new diagnostic technique for uterine endometrial carcinoma :We developed a new method of enzyme immunoassay with MSN-1(EmC-EIA method) and studied its usefulness as a new diagnostic technique for uterine endometrial carcinoma. The positive rate calculated in each group was as follows : 5.7% in the group woth normal uterine endometrium, 20.0% in the group with endometrial hyperplasia, 77.3% in the group of uterine endometrial carcinoma.The combination of our new method and cytodiagnosis performed as a routine clinical examination secured more correct diagnosis and increaed the positive rate up to 89.5% in the group with highly differentiated uterine body carcinoma. Therefore the EmC-EIA method was considered to be usuful as an ancillary diagnostic technique for uterine endometrial carcinoma. Less

本研究的目的是分析子宫内膜和输卵管中的糖脂，通过分析糖脂与细胞外基质的关系，不仅阐明子宫内膜癌的受精和着床机制，而且从糖复合物的角度阐明子宫内膜癌的增殖和转移机制，并建立新的诊断方法。 子宫内膜癌的技术。我们得到以下研究结果：1.子宫内膜和输卵管中糖脂和细胞外基质的分析：在正常子宫内膜中，在增殖期检测到少量的硫苷脂且没有羟基化的中性糖脂神经酰胺。另一方面，在分泌期，硫苷脂显着增加，并且观察到神经酰胺的羟基化。硫脂在整个性周期的输卵管中表达。与自我自杀一样，层粘连蛋白明显与硫苷脂具有高亲和力，当从增殖期转入分泌期时，层粘连蛋白开始增加。 2.高危子宫内膜增生症筛查基础数据的收集：据报道，本实验室的单克隆抗体MSN-1主要识别细胞表面的糖脂，并与糖脂发生反应。 90%的子宫内膜癌标本。观察期间，部分病例子宫内膜增生发展为子宫内膜癌。在发展为子宫内膜癌的病例中通过免疫组织学染色测定MSN-1反应性。子宫内膜增生最终发展为子宫内膜癌组计算的阳性率高于对照组计算的阳性率；囊性增生75%，腺瘤性增生78%，非典型增生100%。这些结果表明，分析MSN-1识别的抗原的表达可能有助于筛查高危子宫内膜增生。3.子宫内膜癌新诊断技术的开发：我们开发了一种MSN-1酶免疫分析新方法（EmC-EIA法），并研究了其作为子宫内膜癌新诊断技术的实用性。 癌。各组计算的阳性率如下：正常子宫内膜组为5.7%，子宫内膜增生症组为20.0%，子宫内膜癌组为77.3%。我们的新方法与常规临床检查中的细胞学诊断相结合，确保了更准确的诊断，使高分化组的阳性率提高到89.5%。 子宫体癌。因此，EmC-EIA 方法被认为可作为子宫内膜癌的辅助诊断技术。较少的

项目成果

Mikio Mikami: "Menstrual cycle-associated expression of 2-hydroxy fatty acyl phytosphingosine-containing GlcCer,LacCer and Gb_3Cer in human uterine endometrium" Bioch.Biophys.Acta. 1125. 104-109 (1992)

Mikio Mikami：“人子宫内膜中含有 2-羟基脂肪酰基植物鞘氨醇的 GlcCer、LacCer 和 Gb_3Cer 与月经周期相关的表达”Bioch.Biophys.Acta。

Nobuyuki Susumu, et al: "Subcellular localization of blood group Le^b carbohydrate antigen(MSN-1-reactive antigen) in endometrial cancer cells." Cancer Res.53. 3643-3648 (1993)

Nobuyuki Susumu 等人：“子宫内膜癌细胞中 Le^b 血型碳水化合物抗原（MSN-1 反应性抗原）的亚细胞定位。”

Kiyoshi Takamatsu: "Structural characteristics of the ceramide of neutral glycoshingolipids in the human female genital tract-their menstrual cycle-associated change in the cervical epithelium and uterine endometrium,and their dissociation in the mucosa o

Kiyoshi Takamatsu：“人类女性生殖道中性糖鞘脂神经酰胺的结构特征——宫颈上皮和子宫内膜中与月经周期相关的变化，以及它们在粘膜中的解离”

野澤志朗: "子宮体癌とモノクローナル抗体-抗子宮体癌モノクローナル抗体“MSN-1"とそのEmC-EIA法への応用-" 臨床病理,臨時増刊. 第94号. 147-160 (1993)

野泽四郎：“子宫癌与单克隆抗体——抗子宫癌单克隆抗体“MSN-1”及其在EmC-EIA方法中的应用”《临床病理学》，特刊第94期。147-160（1993）。

塚崎 克己: "子宮内膜増殖症ーその病態および体癌との関連性ー" KARKINOS. 5. 173-183 (1992)

Katsumi Tsukazaki：“子宫内膜增生 - 其病理学及其与身体癌症的关系”KARKINOS 5. 173-183 (1992)。