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A study on expression mechanisms and functions of a novel adhesion molecule, trophinin

新型粘附分子Trothinin的表达机制和功能研究

基本信息

批准号：
10470348
负责人：
NOZAWA Shiro
金额：
$ 6.53万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1. Biological significance of β1, 4-galactosyltransferase in gynecological cancerWe have established a system that selectively measures the activity ofβ-1, 3-galactosyltransferase (β1, 3GT) andβ-1, 4-galactosyltransferase (β1, 4GT), and we measured the activity of both. We also assessed the expression of β1, 4GTmRNA by the Northern blot analysis. The results revealed higherβ1, 3GT activity thanβ1, 4GT activity in uterineendometrial cancer analysis, and the level of expression ofβ1, 4GTmRNA was lower than in uterine cervix cancer and ovarian cancer. We also transfected humanβ1, 4GTcDNA into a uterine endometrial cancer and ovarian cancer derived cell lines, and established a high-expressionβ1, 4GT line and a low-expression line, and investigated the cellular properties of each. The results showed that the cell proliferation capacity and ability to adhere to the extracellular matrix by the high-expressionβ1,4GT line were greater than in the control, and that the cell proliferation capaci … More ty and the ability of adhere to the extracellular matrix by the low expressionβ1, 4GT line were less than in the control. These findings suggested thatβ1, 4GT may be involved in the properties of uterine endometrial cancer and ovarian cancer cells.2. Expression of the AKT2 gene in gynecological cancerThe product of the recently cloned AKT2 gene has been found to be a cell proliferation signal transmitter, and it has been suggested that the presence or absence of abnormal amplification of the AKT2 gene in pancreatic cancer is associated with disease progression and outcome. We therefore analyzed the expression dynamics of the AKT2 were used as a probe to assess the frequency of AKT2 amplification in ovarian cancer patients by the slot-blot method, with the non-cancerous portion of the same specimen being used as a control. The results revealed abnormal amplification of the AKT2 gene in one of six uterine endometrial cancer cell lines in an analysis using cultured cells, whereas no abnormal amplification of the AKT2 gene was detected among the 25 ovarian cancer patients. Less

1. β1, 4-半乳糖基转移酶在妇科癌症中的生物学意义我们建立了选择性测量β-1, 3-半乳糖基转移酶（β1, 3GT）和β-1, 4-半乳糖基转移酶（β1, 4GT）活性的系统，并测量了两者的活性。我们还通过 Northern blot 分析评估了 β1、4GTmRNA 的表达。结果显示，子宫内膜癌中β1、3GT活性高于β1、4GT活性，且β1、4GTmRNA表达水平低于子宫颈癌和卵巢癌。我们还将人β1、4GTcDNA转染至子宫内膜癌和卵巢癌来源的细胞系中，建立了高表达β1、4GT细胞系和低表达细胞系，并研究了各自的细胞特性。结果显示，高表达β1,4GT细胞系的细胞增殖能力和细胞外基质粘附能力均大于对照，而低表达β1,4GT细胞系的细胞增殖能力和细胞外基质粘附能力低于对照。这些结果提示β1、4GT可能参与子宫内膜癌和卵巢癌细胞的特性。 2． AKT2基因在妇科癌症中的表达最近克隆的AKT2基因产物被发现是一种细胞增殖信号递质，有人提出胰腺癌中AKT2基因异常扩增的存在或不存在与疾病进展和结果相关。因此，我们分析了 AKT2 的表达动态，将其用作探针，通过槽印迹法评估卵巢癌患者中 AKT2 扩增的频率，并使用同一样本的非癌部分作为对照。结果显示，在使用培养细胞进行的分析中，六种子宫内膜癌细胞系之一的 AKT2 基因异常扩增，而在 25 名卵巢癌患者中未检测到 AKT2 基因异常扩增。较少的