A study on expression mechanisms and functions of a novel adhesion molecule, trophinin

1.β1，4-半乳糖基转移酶在妇科肿瘤中的生物学意义我们建立了选择性测定β 1，3-半乳糖基转移酶（β1，3GT）和β 1，4-半乳糖基转移酶（β1，4GT）活性的系统，并测定了两者的活性。用北方印迹法检测β1，4 GT mRNA的表达。结果表明，子宫内膜癌中β1，3GT活性高于β1，4GT活性，而β1，4GT mRNA表达水平低于宫颈癌和卵巢癌。我们还将人β1，4GT cDNA转染到子宫内膜癌和卵巢癌细胞系中，建立了高表达β1，4GT细胞系和低表达β1，4GT细胞系，并研究了它们的细胞特性。结果表明，高表达β1，4 GT细胞系的细胞增殖能力和与细胞外基质的粘附能力均高于对照组，细胞增殖能力与对照组相比有显著性差异。 关于我们 低表达的β1，4 GT系的细胞粘附细胞外基质的能力低于对照组。提示β1，4GT可能参与了子宫内膜癌和卵巢癌细胞的生物学特性. AKT 2基因在妇科癌症中的表达最近克隆的AKT 2基因的产物已被发现是细胞增殖信号传递物，并且已表明胰腺癌中AKT 2基因异常扩增的存在或不存在与疾病进展和结果相关。因此，我们分析了AKT 2的表达动力学，并将其用作探针，以通过狭缝印迹法评估卵巢癌患者中AKT 2扩增的频率，将同一标本的非癌部分用作对照。结果显示，在使用培养细胞的分析中，在六种子宫内膜癌细胞系中的一种中发现了AKT 2基因的异常扩增，而在25名卵巢癌患者中没有检测到AKT 2基因的异常扩增。少

1. Biological significance of β1, 4-galactosyltransferase in gynecological cancerWe have established a system that selectively measures the activity ofβ-1, 3-galactosyltransferase (β1, 3GT) andβ-1, 4-galactosyltransferase (β1, 4GT), and we measured the activity of both. We also assessed the expression of β1, 4GTmRNA by the Northern blot analysis. The results revealed higherβ1, 3GT activity thanβ1, 4GT activity in uterineendometrial cancer analysis, and the level of expression ofβ1, 4GTmRNA was lower than in uterine cervix cancer and ovarian cancer. We also transfected humanβ1, 4GTcDNA into a uterine endometrial cancer and ovarian cancer derived cell lines, and established a high-expressionβ1, 4GT line and a low-expression line, and investigated the cellular properties of each. The results showed that the cell proliferation capacity and ability to adhere to the extracellular matrix by the high-expressionβ1,4GT line were greater than in the control, and that the cell proliferation capaci … More ty and the ability of adhere to the extracellular matrix by the low expressionβ1, 4GT line were less than in the control. These findings suggested thatβ1, 4GT may be involved in the properties of uterine endometrial cancer and ovarian cancer cells.2. Expression of the AKT2 gene in gynecological cancerThe product of the recently cloned AKT2 gene has been found to be a cell proliferation signal transmitter, and it has been suggested that the presence or absence of abnormal amplification of the AKT2 gene in pancreatic cancer is associated with disease progression and outcome. We therefore analyzed the expression dynamics of the AKT2 were used as a probe to assess the frequency of AKT2 amplification in ovarian cancer patients by the slot-blot method, with the non-cancerous portion of the same specimen being used as a control. The results revealed abnormal amplification of the AKT2 gene in one of six uterine endometrial cancer cell lines in an analysis using cultured cells, whereas no abnormal amplification of the AKT2 gene was detected among the 25 ovarian cancer patients. Less