A study on expression mechanisms and functions of a novel adhesion molecule, trophinin

1.β-1，4-半乳糖基转移酶在妇科肿瘤中的生物学意义我们建立了选择性检测β-1，3-半乳糖基转移酶(β-1，3GT)和β-1，4-半乳糖基转移酶(β-1，4GT)的体系，并对两者的活性进行了检测。用Northern印迹法检测β-1、4GTm RNA的表达。结果表明，子宫内膜癌组织中β1，3GT活性高于β1，4GT活性，β1，4GT基因表达水平低于宫颈癌和卵巢癌。我们还将人β-1，4GT基因导入子宫内膜癌和卵巢癌细胞系，建立了β-1，4GT高表达细胞系和低表达细胞系，并对其细胞特性进行了研究。结果表明，高表达β-1，4GT细胞株的细胞增殖能力和与细胞外基质的黏附能力均高于对照组，且细胞增殖能力强于…低表达的β-1、4GT细胞系黏附于细胞外基质的能力和黏附能力均低于对照组。这些发现提示β-1、4GT可能参与了子宫内膜癌和卵巢癌细胞的特性。AKT2基因在妇科肿瘤中的表达最近克隆的AKT2基因的产物被发现是细胞增殖信号的传递者，并提示胰腺癌中AKT2基因异常扩增的存在或缺失与疾病的进展和预后有关。因此，我们分析了AKT2的表达动态，并以同一标本的非癌部分为对照，用狭缝印迹法检测AKT2在卵巢癌患者中的扩增频率。结果显示，在用培养细胞进行的分析中，6个子宫内膜癌细胞系中的一个发现了AKT2基因的异常扩增，而在25例卵巢癌患者中没有发现AKT2基因的异常扩增。较少

1. Biological significance of β1, 4-galactosyltransferase in gynecological cancerWe have established a system that selectively measures the activity ofβ-1, 3-galactosyltransferase (β1, 3GT) andβ-1, 4-galactosyltransferase (β1, 4GT), and we measured the activity of both. We also assessed the expression of β1, 4GTmRNA by the Northern blot analysis. The results revealed higherβ1, 3GT activity thanβ1, 4GT activity in uterineendometrial cancer analysis, and the level of expression ofβ1, 4GTmRNA was lower than in uterine cervix cancer and ovarian cancer. We also transfected humanβ1, 4GTcDNA into a uterine endometrial cancer and ovarian cancer derived cell lines, and established a high-expressionβ1, 4GT line and a low-expression line, and investigated the cellular properties of each. The results showed that the cell proliferation capacity and ability to adhere to the extracellular matrix by the high-expressionβ1,4GT line were greater than in the control, and that the cell proliferation capaci … More ty and the ability of adhere to the extracellular matrix by the low expressionβ1, 4GT line were less than in the control. These findings suggested thatβ1, 4GT may be involved in the properties of uterine endometrial cancer and ovarian cancer cells.2. Expression of the AKT2 gene in gynecological cancerThe product of the recently cloned AKT2 gene has been found to be a cell proliferation signal transmitter, and it has been suggested that the presence or absence of abnormal amplification of the AKT2 gene in pancreatic cancer is associated with disease progression and outcome. We therefore analyzed the expression dynamics of the AKT2 were used as a probe to assess the frequency of AKT2 amplification in ovarian cancer patients by the slot-blot method, with the non-cancerous portion of the same specimen being used as a control. The results revealed abnormal amplification of the AKT2 gene in one of six uterine endometrial cancer cell lines in an analysis using cultured cells, whereas no abnormal amplification of the AKT2 gene was detected among the 25 ovarian cancer patients. Less