A study on expression mechanisms and functions of a novel adhesion molecule, trophinin

1. 我们建立了选择性测定β- 1,3 -半乳糖转移酶（β 1,3gt）和β- 1,4 -半乳糖转移酶（β 1,4gt）活性的系统，并对两者的活性进行了测定。我们还通过Northern blot分析评估了β 1,4 gtmrna的表达。结果显示，β 1,3 gt在子宫内膜癌组织中的活性高于β 1,4 gt, β 1,4 gtmrna的表达水平低于宫颈癌和卵巢癌组织。我们还将人β 1,4 gtcdna转染到子宫内膜癌和卵巢癌衍生细胞系中，建立了高表达的β 1,4 gt细胞系和低表达的β 1,4 gt细胞系，并对其细胞特性进行了研究。结果表明，高表达的β 1,4gt细胞系细胞增殖能力和粘附细胞外基质的能力均高于对照，而低表达的β 1,4gt细胞系细胞增殖能力和粘附细胞外基质的能力均低于对照。这些发现提示β 1,14 gt可能参与了子宫内膜癌和卵巢癌细胞的性质。妇科癌症中AKT2基因的表达最近克隆的AKT2基因的产物已被发现是细胞增殖信号的传递者，并且已经表明在胰腺癌中AKT2基因异常扩增的存在或缺失与疾病的进展和结局有关。因此，我们分析了AKT2的表达动态，并使用slot-blot方法作为探针来评估AKT2在卵巢癌患者中的扩增频率，并将同一标本的非癌部分作为对照。结果显示，在使用培养细胞的分析中，六种子宫内膜癌细胞系中的一种存在AKT2基因异常扩增，而在25名卵巢癌患者中未检测到AKT2基因异常扩增。少

1. Biological significance of β1, 4-galactosyltransferase in gynecological cancerWe have established a system that selectively measures the activity ofβ-1, 3-galactosyltransferase (β1, 3GT) andβ-1, 4-galactosyltransferase (β1, 4GT), and we measured the activity of both. We also assessed the expression of β1, 4GTmRNA by the Northern blot analysis. The results revealed higherβ1, 3GT activity thanβ1, 4GT activity in uterineendometrial cancer analysis, and the level of expression ofβ1, 4GTmRNA was lower than in uterine cervix cancer and ovarian cancer. We also transfected humanβ1, 4GTcDNA into a uterine endometrial cancer and ovarian cancer derived cell lines, and established a high-expressionβ1, 4GT line and a low-expression line, and investigated the cellular properties of each. The results showed that the cell proliferation capacity and ability to adhere to the extracellular matrix by the high-expressionβ1,4GT line were greater than in the control, and that the cell proliferation capaci … More ty and the ability of adhere to the extracellular matrix by the low expressionβ1, 4GT line were less than in the control. These findings suggested thatβ1, 4GT may be involved in the properties of uterine endometrial cancer and ovarian cancer cells.2. Expression of the AKT2 gene in gynecological cancerThe product of the recently cloned AKT2 gene has been found to be a cell proliferation signal transmitter, and it has been suggested that the presence or absence of abnormal amplification of the AKT2 gene in pancreatic cancer is associated with disease progression and outcome. We therefore analyzed the expression dynamics of the AKT2 were used as a probe to assess the frequency of AKT2 amplification in ovarian cancer patients by the slot-blot method, with the non-cancerous portion of the same specimen being used as a control. The results revealed abnormal amplification of the AKT2 gene in one of six uterine endometrial cancer cell lines in an analysis using cultured cells, whereas no abnormal amplification of the AKT2 gene was detected among the 25 ovarian cancer patients. Less