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Development of the method for environmental assessments based on the recognition of the correlation between the speciation of trace metals and the response by living organisms

基于微量金属形态与生物体反应之间相关性的认识开发环境评估方法

基本信息

批准号：
06640785
负责人：
YOKOI Kunihiko
金额：
$ 1.41万
依托单位：
Osaka Kyoiku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

At the early stage of this project fundamentals of the trace metal speciation were investigated and the method for adsorptive collection with Calcein-Blue has been established . Using this method, metal ions were determined sensitively and the limit of detection for cadmium and lead ion were 0.05 and 0.04nM,respectively. For various fresh water samples, the concentrations for above metal ions were directly determined to be 0.2-4 nM with great ease. Secondly, to know each concentration for labile and inert complexes, dissolved organic compounds have to be decomposed before measurements. When the concentrations of metal ions are very low, the decomposition process have to be done under non-contaminated condition. We developed the decomposition method of UV-irradiation with low pressure mercury lamp. With this method , interfering compounds for electrochemical measurements, such as aromatic compounds, humic acids, amino acids, etc, could be decomposed successfully under non-contaminated c … More ondition. Further, we tried to investigate to make the physico-chemical standard to understand the response of living organisms to various metal complexes. Humic acid was represented as the ligand in natural waters. To know the complexing capacity, c_<L1>, and the conditional stability constant, K'_<ML1>, using adsorptive voltammetry, various c_<L1> could be obtained under various values for detection window, logalpha_<ML2>, depends on the ligand for adsorptive collection and its concentraion. For nickle ion, when logalpha_<ML2> was 4.73,5.93 and 6.73 at pH 8.5, c_<L1> was determined to be 14.1,8.7 and 8.3 nM/ppm, and logK'_<ML1>, was 12.2,13.6 and 14.5, respectively. As increasing logalpha_<ML2>, c_<L1> was decreased and logK'_<ML1> was increased. This indicates the functinal groups in humic acids have wide range of ability for coodination. At this stage it has been suggested the possibility that, with logalpha_<ML2>, the response of living organisms to metal ion could be recognized by generalized numerical values. Less

在该项目的早期阶段，研究了痕量金属形态的基本原理，并建立了钙黄绿素蓝吸附收集的方法。该方法对金属离子的测定灵敏，镉和铅离子的检出限分别为0.05和0.04nM。对于各种淡水样品，可轻松直接测定上述金属离子的浓度为0.2-4 nM。其次，为了了解不稳定和惰性复合物的浓度，必须在测量前分解溶解的有机化合物。当金属离子浓度很低时，分解过程必须在无污染的条件下进行。我们开发了低压汞灯紫外线照射分解法。该方法可以在无污染的条件下成功分解电化学测量的干扰化合物，如芳香族化合物、腐殖酸、氨基酸等。此外，我们试图研究制定物理化学标准，以了解生物体对各种金属络合物的反应。腐殖酸被表示为天然水中的配体。为了了解络合能力 c_<L1> 和条件稳定性常数 K'_<ML1>，使用吸附伏安法，可以在不同的检测窗口值 logalpha_<ML2> 下获得不同的 c_<L1>，这取决于吸附收集的配体及其浓度。对于镍离子，当pH 8.5时logalpha_<ML2>为4.73、5.93和6.73时，c_<L1>测定为14.1、8.7和8.3nM/ppm，logK'_<ML1>分别为12.2、13.6和14.5。随着 logalpha_<ML2> 的增加，c_<L1> 减少，logK'_<ML1> 增加。这表明腐殖酸中的功能基团具有广泛的配位能力。在这个阶段，有人提出了这样的可能性：利用 logalpha_<ML2>，可以通过广义数值来识别活体对金属离子的响应。较少的