Commercialization of Enzyme Modified Carbon-Fiber Electrodes Paired with Voltammetry for Simultaneous Real-Time Monitoring of Electroactive and Non-Electroactive Species at Discrete Brain Locations

摘要 商业上可获得的生物传感器被设计成在给定记录下一次仅测量一个分子 绝佳的价钱这是一个问题，因为大脑中的化学信号不是孤立地工作的，而是神经传递 涉及许多化学物质同时释放， 神经化学物质相互之间的波动。理解这些关系对于 开发用于多种神经系统疾病的药物和治疗方法。The Sombers实验室 建立了使用快速扫描循环伏安法（FSCV）和碳纤维微电极进行 同时检测快速多巴胺波动和非电活性物质，如 葡萄糖，在相同的记录位置。这是以比目前更高的空间和时间分辨率完成的 可用的方法。本实验室的目标市场：大脑和行为研究SBIR的工具是 将北卡罗来纳州的Sombers团队开发的技术转化并商业化 大学第一个目标是将多巴胺和葡萄糖联合检测的核心技术从 将Sombers实验室转让给Pinnacle Technology，该公司开发、制造和销售 生物传感器和电化学测量系统。Pinnacle生产的传感器将完全 该技术的特征和详细规格（灵敏度、线性范围、保质期和基准 对于体内性能）将被概述。第二个目标是开发培训工具和软件， 与FSCV的正确实施、表征和分析相关的学习曲线 研究或临床前应用。这将通过修改现有的Pinnacle软件来实现， 创建一个直观的平台，用于使用商业探针采集和分析伏安法数据。 最后，高生产价值的培训视频将被创建，并免费提供的顶峰 网站这些将详细的实验程序的所有方面，在体内伏安法，包括探针 校准、外科手术以及数据采集和分析协议。总的来说，这个项目是创新的， 因为它通过利用酶促产生的H2O2固有的氧化还原活性而脱离现状 为了鉴定目标非电活性物质，即使在存在通常 排除干扰物。它是重要的，因为它结合了两个国家的最先进的和良好的特点， 神经化学监测技术，以一种聪明、直接和前所未有的方式， 该社区拥有一个既定的工具，可用于研究葡萄糖在复杂的生理过程中的作用， 从基本的内分泌功能到动机。它有望对人类社会产生变革性影响， 通过让研究人员对大脑功能的各个方面感兴趣， 这些特定的神经化学物质在离散的大脑位置中快速地共同波动。

ABSTRACT Commercially available biosensors are designed to measure only one molecule at a time at a given recording site. This is a problem because chemical signals in the brain do not work in isolation; rather, neurotransmission involves many chemical species simultaneously released and little is known about how specific neurochemicals fluctuate relative to one another. Understanding these relationships is critical to the development of drugs and treatments for a wide range of neurological disorders. The Sombers Lab has established the feasibility of using fast-scan cyclic voltammetry (FSCV) and carbon-fiber microelectrodes for the simultaneous detection of rapid dopamine fluctuations and those of non-electroactive species, such as glucose, at the same recording site. This is done with higher spatial and temporal resolution than currently available methods. The goal of this Lab to Marketplace: Tools for Brain and Behavioral Research SBIR is to translate and commercialize the technology developed by the Sombers team at North Carolina State University. The first goal is to transfer the core technology for the co-detection of dopamine and glucose from the Sombers laboratory to Pinnacle Technology, a company that has developed, manufactured and sold biosensors and electrochemical measurement systems worldwide. Pinnacle-produced sensors will be fully characterized and detailed specifications for the technology (sensitivity, linear range, shelf-life and benchmarks for in vivo performance) will be outlined. The second goal is to develop training tools and software to minimize the learning curve associated with the proper implementation, characterization and analysis of FSCV in research or pre-clinical applications. This will be accomplished by modifying existing Pinnacle software to create an intuitive platform for acquisition and analysis of voltammetry data using the commercial probes. Finally, high production value training videos will be created and made freely available on the Pinnacle website. These will detail experimental procedures for all aspects of in vivo voltammetry including probe calibration, surgical procedures, and data acquisition and analysis protocols. Overall, this project is innovative, because it departs from the status quo by utilizing the redox activity inherent to enzymatically generated H2O2 to identify targeted non-electroactive species, even in the presence of electroactive molecules that are typically excluded as interferents. It is significant, because it combines two state-of-the-art and well-characterized technologies for neurochemical monitoring in a clever, straightforward, and unprecedented manner to provide the community with an established tool that can be used to study the role of glucose in complex physiological processes ranging from basic endocrine function to motivation. It promises to have a transformative effect on neuroscience by allowing researchers interested in diverse aspects of brain function to better understand how these specific neurochemicals rapidly co-fluctuate in discrete brain locations.