Metabolism of Carotenoids by Rumen Microbes

瘤胃微生物对类胡萝卜素的代谢

• 批准号: 06660361
• 负责人: TOMITA Yoshifumi
• 金额: $ 1.28万
• 依托单位: Miyazaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06660361/
• 关键词: rumen microbe; rumen protozoa; rumen bacteria; carotene; carotenoid; metabolism; 栄養代謝; 高速液体クロマトグラフィ

1 Carotenes and carotenoids in rumen fluids from fistulated ruminants (cattle and goats) were assayd by using high-performance liquid chromatography (HPLC). Xanthophylls and betacarotene were rich in protozoa but not in bacteria in rumen fluids. Carotenoid types and contents in feeds mainly influenced rumen carotenoid characteristics.2 Several types of carotenoids (hydrocarbon, keto-, hudroxy-carotenoids) added into rumen fluids containing bacteria, protozoa or their mixture were partly modified after incubation. The survival rate and the growth of protozoa were not effected by carotenoids used. Hydrogenation of unsaturated bonding of carotenoids in rumen fluids was suggested.3 Some unidentified peaks different from those in feeds were detected in rumen fluids and microbes suggesting the metabolism or modification of carotenoids.4 Assay methods for carotenoids in rumen fluids and microbes were improved as to precision and accuracy.

1用高效液相色谱法测定了反刍动物(黄牛和山羊)瘤胃液中的胡萝卜素和类胡萝卜素。瘤胃液中叶黄素和β-胡萝卜素富含原生动物，但不含细菌。饲料中类胡萝卜素的种类和含量主要影响瘤胃类胡萝卜素的特性。2.添加到含有细菌、原生动物或它们的混合物的瘤胃液中的几种类胡萝卜素(碳氢类、酮类、胡萝卜素)在培养后被部分修饰。类胡萝卜素的使用不影响原生动物的存活率和生长。提出了瘤胃液中类胡萝卜素不饱和键的氢化反应。3在瘤胃液和微生物中检测到与饲料中不同的峰，提示类胡萝卜素的代谢或修饰。4改进了瘤胃液和微生物中类胡萝卜素的检测方法，提高了精密度和准确度。

项目成果

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Sakurada, M.：“使用乙二醇和瘤胃液冷冻保存厌氧瘤胃真菌的简单方法。”FEMS 微生物学快报 127・2 (1995)。

Sakuraba.M.: "Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1." FEMS Microbiology Letters. 137・2. 75-78 (1996)

Sakuraba.M.：“来自 Piromyces communis OTS1 的胞质几丁质酶的纯化和特征。”FEMS 微生物学快报 137・2（1996 年）。

アミン、他2名: "Rapid determination of phenylalanine and its related compounds in rumen fluid by HPLC." J.Chromatography B :. 663. 201-207 (1995)

Amin 和其他 2 人：“通过 HPLC 快速测定瘤胃液中的苯丙氨酸及其相关化合物。”J.Chromatography B：663. 201-207 (1995)

Morgavi.D.P.: "Electrophoretic forms of Chitinolytic and lysozyme activities in ruminal protozoa." Current Microbiology. 32・3. 115-118 (1996)

Morgavi.D.P.：“瘤胃原生动物中几丁质分解和溶菌酶活性的电泳形式”，《当代微生物学》115-118。

Amin, M.R.et al.: "Rapid determination of phenylalaninc and its related compounds in rumen fluid by high-performance liquid chromatography." J.Chromatography B.663-2. 201-207 (1995)

Amin, M.R.等人：“通过高效液相色谱法快速测定瘤胃液中的苯丙氨酸及其相关化合物。”