アメロゲニン遺伝子をターゲットとする遺伝子の検討

针对牙釉蛋白基因的基因检查

• 批准号: 06771629
• 负责人: 大山 和彦
• 金额: $ 0.64万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06771629/
• 关键词: アメロゲニン遺伝子; HGF; TGF-β; エナメル質形成不全ラット

本研究では,アメロゲニン遺伝子に先立って発現し,形態形成に関与している2種の成長因子Hepatocyte growth factor(以下HGF),Transforming growth factor-β1(TGF-β)の遺伝子発現をin situ hybridization法を用いて,正常ラットとエナメル質形成不全ラット(不全ラット)において検討した。胎生13日から生後2日のwister系ラット(正常ラット)と不全ラットの下顎M1の歯胚を観察した。その結果TGF-βのsignalは,HGFよりも早い時期,すなわちbud stage(13日)でエナメル器および歯乳頭に認めた。cap-stage(14,5日)では,強いシグナルをエナメル器および歯乳頭に認めた。bell stage(16,17日)になりHGFのsignalを歯乳頭に限局して発現を認めた。TGF-βのsignalはエナメル器の発現は弱くなっていたが,歯乳頭では強い発現が観察された。HGFのsignalは,以後咬頭部より消退し始め,20日から生後1日にかけて消失した。TGF-βのsignalも生後1-2日には徐々に消失した。歯の発生過程でTGF-β遺伝子は,アメロゲニン遺伝子,HGF遺伝子に先立って発現し,生後1-2日目まで発現を認めた。このことよりTGF-β遺伝子は歯を構成する細胞の増殖分化に深く関わっていることが示唆された。しかし正常,不全ラットにおいて特に発現の相違はなく,不全ラットの病因遺伝子は今後の研究が待たれる。

In this study, the growth factor Hepatocyte growth factor (below HGF), Transforming growth factor- β 1 (TGF- β) and in situ hybridization method were used in the study. The results showed that the in situ hybridization method was used in the study, and the results showed that the growth factors of shape formation and growth factor were the growth factor of the growth factor (below). The fetus was born on the 13th and 2nd day after birth, and the embryo was examined under the M1 embryo. The results showed that TGF- β was signal, HGF was in early morning, and the bud stage (13th) was checked to check the breast cancer confirmation. Cap-stage (14,5), make sure that the equipment is in good condition. Bell stage (16,17) to HGF the signal to obtain confirmation from the Bureau of restriction. The TGF- β-signal device shows that it is weak, and the breast cancer is strong. After HGF, the signal will disappear, and on the 20th, it will disappear on the 1st day. TGF- β "signal" disappeared 1-2 days after birth. During the course of birth, the TGF- β child was born, and the HGF child was first established. After 1-2 days of life, the child was recognized. The cells of TGF- β were divided into colonies, and the differentiation of colonies was deep. The disease is normal, but it is not completely clear that the cause of the disease is abnormal, and that the etiology will be studied in the future.