A novel hair analysis for trace elements on hair cross section by synchrotron radiation X-ray fluorescence imaging with X-ray microprobe.

利用 X 射线微探针同步加速器辐射 X 射线荧光成像对头发横截面的微量元素进行新型头发分析。

• 批准号: 06670372
• 负责人: SHIMOJO Nobuhiro
• 金额: $ 1.34万
• 依托单位: The University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06670372/
• 关键词: SR-XRF; smelter; hair; heavy metals; biological monitoring; time lag; SOD; methylmercury; 金属精錬工場; 非破壊放射光蛍光X線分析; 2次元イメージング; 金属精錬労働者; 金属; 金属精錬; 労働者; 生物学的モニタリング; 鉛; 亜鉛; 銅

The result of the studies carried out for three years, 1994 to 1996, can be summarized as follows.1) In this study, we attempted to perform the X-ray microanalysis, ie, one pixel is 5*5mum^2, to reveal trace element distribution of human hair cross sections without washing the samples. The SR-XRF imaging technique used in this study clearly revealed differential distribution of Zn and Pb in the cross hair secton of the smelter.2) A study of alterations in mammalian Cu, Zn- and Mn-superoxide dismutase caused by methylmercury has been reviewed. Mechanisms for the isozyme-elective decrease in Mn-SOD by the organometallic compound observrd in vivo has been extensively examined by experiments with purified enzyme preparations.3) The metal leveals in blood and hair reached a maximum at 7 and 8.5 days, respectively, but these elimination curves exhibited a similar pattern (r=0.97) with a time lag (about 3.5 days).

1994 ~ 1996年三年的研究结果如下：1）本研究尝试用X射线微区分析法，即一个象素为5*5mum^2，在不清洗样品的情况下，揭示人发横截面的微量元素分布。本研究中使用的SR-XRF成像技术清楚地揭示了锌和铅在冶炼厂十字线部分的差异分布。2）已审查了由甲基汞引起的哺乳动物铜、锌和锰超氧化物歧化酶变化的研究。3）血和发中金属离子的清除量分别在第7天和第8.5天达到最大值，但它们的清除曲线具有相似的规律（r=0.97），但有一个时滞（约3.5天）。

项目成果

S.Homma-Takeda, M.Ishido, Y.Kumagai, Y.Takenaka, and N.Shimojo.: "In situ determination of mercury distribution and apoptotic cells in mercury-treated rat kidney by a combination of synchrotron radiation X-ray fluorescence (SR-XRF) imaging and TUNEL stain

S.Homma-Takeda、M.Ishido、Y.Kumagai、Y.Takenaka 和 N.Shimojo.：“通过同步辐射 X 射线荧光组合原位测定汞处理的大鼠肾脏中的汞分布和凋亡细胞

N.Shimojo, I.Matsuzaki, S.Homma-Takeda, K.Tabata, M.Shinyashiki, G.Sun, A.Iida and Y.Kumagai.: "A novel hair analysis for trace elements on hair cross section by synchrotron radiation X-ray fluorescence imaging with X-ray microscope : Two-dimentional dist

N.Shimojo、I.Matsuzaki、S.Homma-Takeda、K.Tabata、M.Shinyashiki、G.Sun、A.Iida 和 Y.Kumagai.：“通过同步辐射对头发横截面微量元素进行新颖的头发分析

新井 淑弘: "無機水銀投与による急性腎障害に及ぼす運動負荷の影響" 産衛誌. 37. 195-196 (1995)

Yoshihiro Arai：“运动负荷对无机汞给药引起的急性肾损伤的影响”Sanei Journal 37. 195-196 (1995)。

Shino Homma: "Site-specific distribution of copper,selenium,and zinc in human kidney by synchrotron radiation induced X-ray fluorecence." Nuclear Instruments and Methods in Physics Research B. 103. 229-232 (1995)

Shino Homma：“通过同步加速器辐射诱导 X 射线荧光观察人体肾脏中铜、硒和锌的位点特异性分布。”

Nobuhiro Shimojo: "Effects of exercise traning on the distribution of metallic mercury in mice." Human & Experimental Toxicology. 13. 524-528 (1994)

Nobuhiro Shimojo：“运动训练对小鼠体内金属汞分布的影响。”