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Neuronal control of Cerebrovascular Bed

脑血管床的神经元控制

基本信息

批准号：
06671420
负责人：
MAEDA Minoru
金额：
$ 1.41万
依托单位：
JUNTENDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

There are two possible causes for the development of plateau waves : persistent intracranial hypertension and cerebral vasomotor reaction. Previously we investigated the function of noradrenergic cell group (locus coeruleus complex, LC ; medullary reticular formation, MRF) and the cholinoceptive pontine area (CPA) in the generation of plateau waves. This study investigated the function of the cholinergic basal forebrain in control of ICP and cerebrovascular bed.Ten chloralose-anesthetized-immobilized cats with Kaolin-induced hydrocephalus and six normal rats were used of the experiments. BP,ICP and ETCO_2 were measured continuously. The CBF was measured by the hydrogen clearance method. The first experiments recorded single unit spikes continuously during ICP・variations using tungsten-microelectrode in the dorsomedial hypothalamic nucleus (DMH) of the cats. In the second series of experiments injection needles were inserted for microinjection of glutamate or Ach into the magnocellular … More basal nucleus (nucleus basalis of Meynert, NBM ; substantia innominata, SI) of the rats or the DMH of the cats.Glutamate or Ach microinjection into the NBM and SI caused consistent increases in ICP associated with slightly decreased BP,Ach microinjection into the DMH produced a persistent increase in ICP with a marked decrease in BP.ETCO_2 concentration changed little. Ach microinjection into the NBM or the DMH also induced consistent increases in CBF in the cerebral cortex. The DMH-neuron spike activities increased before onset and during spontaneous ICP elevation. The firing rate of the DMH-neuron discharge was increased in phase with the plateau wave like ICP-variations elicited by microinjection of Ach into the CPA or the contralateral DMH.However, some DMH-neurons demonstrated no suppression or promotion of spike activities during increased ICP.Plateau waves may result from an increase in CBV.Glutamate or Ach induced ICP changes in the present study have resulted from increased CBV in response to reduced cerebral vaso-constrictor tone. Our previous and present results suggest that activity within the cholinergic basal forebrain (NBM,SI,DMH), CPA,LC and MRF contributes to ICP changes and is the intrinsic neuronal origins controlling cerebrovascular bed. Less

高原波的形成可能有两种原因：持续性颅内高压和脑血管反应。以前，我们研究了去甲肾上腺素能细胞群（蓝斑复合体，LC ;延髓网状结构，MRF）和胆碱能脑桥区（CPA）在产生平台波的功能。本实验用氯醛糖麻醉制动的猫10只和正常大鼠6只，观察了基底前脑胆碱能神经在颅内压（ICP）和脑血管床控制中的作用。连续监测血压（BP）、颅内压（ICP）和呼气末二氧化碳分压（ETCO_2）。采用氢清除法测定脑血流量。第一个实验用钨微电极在猫下丘脑背内侧核（DMH）连续记录到ICP·变化过程中的单个单位峰电位。在第二组实验中，插入注射针，将谷氨酸或Ach微量注射到大细胞内。 ...更多信息在大鼠的Meynert基底核（NBM）和猫的DMH内微量注射谷氨酸或乙酰胆碱（Ach），均引起颅内压持续升高，血压轻度下降，DMH内微量注射乙酰胆碱（Ach）则引起颅内压持续升高，血压显著下降，ETCO_2浓度变化不大。乙酰胆碱微量注射到NBM或DMH也诱导一致的增加，在大脑皮层的CBF。DMH神经元的放电活动在发病前和自发性ICP升高时增加。在CPA或对侧DMH中微量注射Ach，DMH神经元放电频率增加，与ICP的平台波样变化同相。一些DMH-在本研究中，谷氨酸或乙酰胆碱引起的颅内压变化是由于CBV增加引起的，而CBV增加是由于谷氨酸或乙酰胆碱引起的颅内压降低引起的。脑血管收缩张力。我们以前和现在的研究结果表明，胆碱能基底前脑（NBM，SI，DMH），CPA，LC和MRF内的活动有助于ICP的变化，是控制脑血管床的内在神经元来源。少