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Inhibition of Ocular Neovasvularization : An Experimental Study

抑制眼部新生血管形成：实验研究

基本信息

批准号：
06671767
负责人：
ISHIBASHI Tatsuro
金额：
$ 1.41万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671767/
关键词：
New vessels Neovascularization Endothelial cells Pericytes In vitro In vivo Retinal vessels

项目摘要

1.Pericytes of newly formed vessels in experimental choroidal neovascularization.We examined the role of pericytes in the progression of choroidal neovascularization in a primate model. Choroidal neovascularization was induced by intense laser photocoagulation in monkey eyes. The eyes were enucleated and were studied with electron microscopy. Pericytes were involved maturation of the endothelial cells that formed choroidal new vessels.2.Effect of tecogalan sodium on angiogenesis in vitro by choroidal endothelial cells.We examined the possible inhibitory effect of tecogalan sodium on three important components of in vitro angiogenesis (endothelial proliferation, migration, and tube formation) using bovine choroidal endothelial cells (CECs). Basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and a combination of bFGF and VEGF stimulated proliferation, migration, and tube formation by CECs in vitro. These stimulatory effects were inhibited by tecogalan sodiu … More m.3.Tecogalan sodium inhibits comeal neovascularization induced by basic fibroblast growth factor.The antiangiogenic effect of tecogalan sodium on corneal neovascularization was investigated. Comeal neovascularization of rabbits induced by bFGF was inhibited by tecogalan sodium in a dose-dependent manner.4.Vessel formation by choroidal endothelial cells in vitro is modulated by retinal pigment epithelial cells.We elucidated the mechanism of bovine CEC angiogenesis and, in particular, the role of retinal pigment epithelial (RPE) cells by use of an in vitro coculture assay system. RPE cells showed to have angiogenic and antiangiogenic effects on CECs. VEGF anf bFDF playd an important role in this phenomenon.5.Inhibitory effect of vitamin E succinate on the proliferation of cultured bovine choroidal endothelial cells.We reported the effect of vitamin E (VE) succinate on the proliferation of cultured bovine CECs. VE succinate was found to significantly inhibit CEC proliferation. The protein kinase C (PKC) stimulator phorbol ester reduced the VE succinate-induced inhibitation od bovine CEC proliferation. PKC was involved in this action at least in part. Less

1.实验性脉络膜新生血管中新形成血管的周细胞：我们在灵长类动物模型中研究了周细胞在脉络膜新生血管进展中的作用。强激光光凝法诱导猴眼脉络膜新生血管形成。摘除眼球，并进行电子显微镜研究。2.替考加兰钠对脉络膜内皮细胞体外血管生成的影响本实验以牛脉络膜内皮细胞（covine choroidal endothelial cells，CEC）为实验材料，研究了替考加兰钠对体外血管生成的三个重要组成部分（内皮细胞增殖、迁移和管腔形成）的可能抑制作用。碱性成纤维细胞生长因子（bFGF），血管内皮生长因子（VEGF），以及bFGF和VEGF的组合刺激体外CEC的增殖，迁移和管形成。这些刺激作用被tecogalan sodiu所抑制 ...更多信息 m.3.替可加兰钠抑制碱性成纤维细胞生长因子诱导的角膜新生血管的形成。tecogalan钠可剂量依赖性地抑制bFGF诱导的兔角膜新生血管的形成。4.视网膜色素上皮细胞（retinal pigment epithelial，RPE）对体外培养的脉络膜内皮细胞血管形成有调节作用。RPE细胞对CEC具有促血管生成和抗血管生成作用。5.维生素E琥珀酸酯对体外培养的牛脉络膜内皮细胞增殖的抑制作用本文报道了维生素E琥珀酸酯对体外培养的牛脉络膜内皮细胞增殖的影响。发现VE琥珀酸酯显著抑制CEC增殖。蛋白激酶C（PKC）刺激剂佛波酯降低VE琥珀酸酯诱导的牛CEC增殖的抑制作用。PKC至少部分参与了这一作用。少