Inhibition of Ocular Neovasvularization : An Experimental Study

1.实验性脉络膜新生血管中新生血管的周细胞。我们在灵长类动物模型中研究了周细胞在脉络膜新生血管形成过程中的作用。强激光光凝诱导猴眼脉络膜新生血管形成。取眼球去核，电镜观察。周细胞参与内皮细胞的成熟，形成脉络膜新血管。地高聚糖钠对脉络膜内皮细胞体外血管生成的影响。我们用牛脉络膜内皮细胞（CECs）研究了特高alan钠对体外血管生成的三个重要组成部分（内皮细胞增殖、迁移和管形成）可能的抑制作用。碱性成纤维细胞生长因子（bFGF）、血管内皮生长因子（VEGF）以及bFGF和VEGF的组合刺激体外CECs的增殖、迁移和成管。这些刺激作用被高聚糖钠所抑制。替高格兰钠抑制碱性成纤维细胞生长因子诱导的眼部新生血管。研究了特高聚糖钠对角膜新生血管的抗血管生成作用。3 .特高聚糖钠对bFGF诱导兔角膜新生血管的抑制作用呈剂量依赖性。脉络膜内皮细胞体外血管形成受视网膜色素上皮细胞调节。我们通过体外共培养系统阐明了牛CEC血管生成的机制，特别是视网膜色素上皮细胞（RPE）的作用。RPE细胞对CECs有血管生成和抗血管生成作用。VEGF和bFDF在这一现象中发挥了重要作用。琥珀酸维生素E对培养的牛脉络膜内皮细胞增殖的抑制作用。我们报道了琥珀酸维生素E （VE）对培养的牛CECs增殖的影响。发现琥珀酸VE明显抑制CEC增殖。蛋白激酶C （PKC）刺激剂酚酯降低了琥珀酸VE诱导的对牛CEC增殖的抑制作用。PKC至少部分参与了这一行动。少

1.Pericytes of newly formed vessels in experimental choroidal neovascularization.We examined the role of pericytes in the progression of choroidal neovascularization in a primate model. Choroidal neovascularization was induced by intense laser photocoagulation in monkey eyes. The eyes were enucleated and were studied with electron microscopy. Pericytes were involved maturation of the endothelial cells that formed choroidal new vessels.2.Effect of tecogalan sodium on angiogenesis in vitro by choroidal endothelial cells.We examined the possible inhibitory effect of tecogalan sodium on three important components of in vitro angiogenesis (endothelial proliferation, migration, and tube formation) using bovine choroidal endothelial cells (CECs). Basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and a combination of bFGF and VEGF stimulated proliferation, migration, and tube formation by CECs in vitro. These stimulatory effects were inhibited by tecogalan sodiu … More m.3.Tecogalan sodium inhibits comeal neovascularization induced by basic fibroblast growth factor.The antiangiogenic effect of tecogalan sodium on corneal neovascularization was investigated. Comeal neovascularization of rabbits induced by bFGF was inhibited by tecogalan sodium in a dose-dependent manner.4.Vessel formation by choroidal endothelial cells in vitro is modulated by retinal pigment epithelial cells.We elucidated the mechanism of bovine CEC angiogenesis and, in particular, the role of retinal pigment epithelial (RPE) cells by use of an in vitro coculture assay system. RPE cells showed to have angiogenic and antiangiogenic effects on CECs. VEGF anf bFDF playd an important role in this phenomenon.5.Inhibitory effect of vitamin E succinate on the proliferation of cultured bovine choroidal endothelial cells.We reported the effect of vitamin E (VE) succinate on the proliferation of cultured bovine CECs. VE succinate was found to significantly inhibit CEC proliferation. The protein kinase C (PKC) stimulator phorbol ester reduced the VE succinate-induced inhibitation od bovine CEC proliferation. PKC was involved in this action at least in part. Less