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Study on screening method for allergy associated with dental metals

牙科金属过敏筛查方法研究

基本信息

批准号：
06672068
负责人：
YAMANAKA Sumie
金额：
$ 1.34万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06672068/
关键词：
Dental Metals Allergy Dermal Sensitization Test Patch Test Mouse Ear Swelling Test

项目摘要

Various dental metals have been used as restrative, prosthetic and endodontic materials, but there have been many case reports of contact allergy associated with dental metals. Then the screening methods to evaluate simply for allergy related to dental metals in humans and experimental animals were investigated and the following results were obtained. *1* 839 normal subjects were patch tested with 16 metal compounds and urushiol. The incidence of positive reaction to each metal was lower than that of urushiol (10.8%). Positive reaction to mercury was 7.7%, which ranked first in dental metals in this study. The order of positivity was Hg>Co>Zn>Sn>Mn>Ni>Au>Pd>In>Cr. Positive reaction to cobalt, nickel and zinc was significantly higher in females than in males. This might be due to the fact that females have more contact with these metals through cosmetics and metal accessories. Positive reaction to metals was high in persons who had experienced allergy to pollen, food or drugs. These findings suggested that persons with allergies were also sensitive to dental metals. *2* Two sesitization tests were compared in guinea-pig. In Maximization method with high sensitivity, Cr, Sn and Ag showed positive reaction. Hg, however, was negative reaction because epidermal necrosis by FCA (Freund's Complete adjuvant) injection perhaps injured immunocyte. On the contrary, Hg exposed positive sensitization reaction in Buehler test, and Cr, Sn and Ag were negative. *3* Mouse ear swelling test (MEST) was simple screening method for detecting sensitization by ear thickness. Hg, Cr and Ni did not show positive reaction by one time induction, and they needed the repeat inductions.It should be shown in future studies that a simple screening method in vivo and in vitro might be useful for the evaluate of hypersensitivity to dental metals.

各种牙科金属材料已被用作参考，修复和牙髓材料，但有许多病例报告与牙科金属材料相关的接触性过敏。然后研究了在人类和实验动物中简单评估与牙科金属相关的过敏的筛选方法，并获得了以下结果。*1* 839名正常受试者接受了16种金属化合物和漆酚的斑贴试验。各金属的阳性反应率均低于漆酚（10.8%）。对汞的阳性反应率为7.7%，在本研究的牙科金属中居首位。正性顺序为Hg>Co>Zn>Sn>Mn>Ni>Au>Pd>In>Cr。女性对钴、镍、锌的阳性反应明显高于男性。这可能是因为女性通过化妆品和金属配件与这些金属接触更多。对花粉、食物或药物过敏的人对金属的阳性反应率很高。这些发现表明，过敏者也对牙科金属敏感。*2* 在豚鼠中比较了两种致敏试验。灵敏度高的最大化法对铬、锡、银呈阳性反应。Hg则为阴性反应，可能是由于FCA（弗氏完全佐剂）引起的表皮坏死损伤了免疫细胞。而汞在Buehler试验中呈阳性反应，铬、锡、银呈阴性反应。*3* 小鼠耳肿胀试验（MEST）是通过耳厚度检测致敏性的简单筛选方法。Hg、Cr、Ni单次诱导均未出现阳性反应，需反复诱导，提示在今后的研究中，一种简便的体内外筛选方法可能有助于评价牙科金属过敏症。