Screening methods for allergy associated with dental metals

牙科金属过敏的筛查方法

• 批准号: 11672057
• 负责人: YAMANAKA Sumie
• 金额: $ 2.37万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672057/
• 关键词: Metal allergy; Dental metals; Lymphocyte stimulation test; Dermal sensitization test; Patch test; MEST (Mouse Ear Swelling Test); γ-インターフェロン; インターロイキン-2; アレルギー発現; スクリーニング検査; 感作性

Many cases of contact allergy due to dental metals have been reported, and several in vitro or in vivo tests have been used for screening of the allergenic epitopes. So we evaluated the mercury sensitization by Mouse Ear Swelling Test (MEST). And determined the lymphocyte stimulation for screening method of metal allergy in humans, and the following results were obtained. (1) MEST was a simple screening method for detecting sensitization of metals by mouse ear thickness. Positive response of allergy was obtained in mercury levels caused light swelling. But any positive sensitization response was not seen in case of repeat induction with non-inflammatory low concentration, or in case of high level induction with skin damage. This depression of mercury sensitization might be caused by immunological tolerance. (2) The lymphocyte sitmulation tests of mercury, nickel, chromium, cobalt, gold and titanium were performed in subjects with or without experience of metal allergy, Cobalt, chromium … More , gold and titanium caused positive proliferation in patch test-positive subjects, and the stimulation index (SI) values were lower than 180 in patch test-negative subjects. But in case of mercury and nickel, the SI values of all subjects were higher than 181. So the T-cells of all subjects might be sensitized to mercury and nickel because those metals were used widely in daily tools. The positive response in the lymphocyte stimulation test might also be caused by immunological tolerance, even if the subjects showed a negaive response in vivo. Therefore the lymphocyte stimulation test was not a sufficiently accurate method in mercury and nickel hupersensitivity. (3) The functional test of lymphocyte cells was determined after activation with metal antigen. Cell surface differentiation antigen CD4 (Helper subset) and CD8 (Suppressor subset) of T-lymphocyte did not show many changs, but cell surface Interleukin-2 (IL-2) receptor was increased in lymphocyte with allergy experience. Then the y-Interferon (y-IFN) and IL-2 activity in culture supernatant were increased extremely in subjects with positive reaction in vivo. So these lymphocyte functions might be able to distinguish the allergy expression from depression caused by immunological tolerance. It should be shown in future studies that these test might be useful for the screening method of hypersensitivity to dental metals. Less

许多病例的接触过敏，由于牙科金属已被报道，并在体外或体内试验已被用于筛选过敏原表位。因此，我们采用小鼠耳肿胀试验（MEST）评价汞致敏性。并确定了淋巴细胞刺激性作为人体金属过敏筛选方法，得到以下结果。(1)MEST是一种简单的用耳厚检测金属致敏性的筛选方法。在汞水平引起的轻度肿胀中获得了过敏的阳性反应。但在无炎症的低浓度重复诱导或在皮肤损伤的高水平诱导的情况下，未观察到任何阳性致敏反应。这种汞致敏抑制可能是免疫耐受所致。(2)对有或无金属过敏史的受试者进行汞、镍、铬、钴、金和钛的淋巴细胞刺激试验， ...更多信息 在斑贴试验阳性受试者中，金和钛引起阳性增殖，斑贴试验阴性受试者的刺激指数（SI）值低于180。但在汞和镍的情况下，所有受试者的SI值均高于181。因此，所有受试者的T细胞可能对汞和镍敏感，因为这些金属广泛用于日常工具。淋巴细胞刺激试验的阳性反应也可能是由免疫耐受引起的，即使受试者在体内显示阴性反应。因此，淋巴细胞刺激试验不是一种足够准确的汞和镍过敏的方法。(3)用金属抗原活化淋巴细胞后进行淋巴细胞功能测定。T淋巴细胞表面分化抗原CD_4（辅助亚群）和CD_8（抑制亚群）无明显变化，但有变态反应史的淋巴细胞表面IL-2受体表达增加。体内试验阳性受试者培养上清中γ-干扰素（γ-IFN）和IL-2活性显著升高。因此，这些淋巴细胞功能可能能够区分过敏表达和免疫耐受引起的抑郁。在今后的研究中，这些试验可能是有用的牙科金属过敏症的筛选方法。少

项目成果

野村登志夫, 山中すみへ 他: "培養細胞を用いた歯科用素材の安全性評価"歯科学報. 99. 245 (1999)

Toshio Nomura、Sumie Yamanaka 等人：“使用培养细胞对牙科材料进行安全性评估”《牙科杂志》99. 245 (1999)。

山中すみへ, 野村登志夫 他: "歯科用素材の細胞毒性評価"口腔衛生学会雑誌. 49. 642-643 (1999)

Sumie Yamanaka、Toshio Nomura 等：“牙科材料的细胞毒性评估”口腔健康协会杂志 49. 642-643 (1999)。

Yamanaka Sumie: "Screening assessment of acute toxicity of chemicals by using cultured cell lines"Alt. Animal Testing and Experimentation. Vol. 7. 115-116 (2001)

山中纯惠：“使用培养细胞系筛选评估化学品的急性毒性”Alt。

Yamanaka Sumie et al.: "The lymphocyte stimulation method for allergy associated with dental metals"J. Dental Health. Vol. 51. 69-76 (2001)

Yamanaka Sumie 等：“用于与牙科金属相关的过敏的淋巴细胞刺激方法”J.

山中すみへ, 太田 薫 他: "ヒトにおける金属アレルギーのスクリーニング法としてのリンパ球刺激試験"口腔衛生学会雑誌. 51. 69-76 (2001)

Sumie Yamanaka、Kaoru Ota 等人：“淋巴细胞刺激试验作为人类金属过敏的筛查方法”口腔健康协会杂志 51. 69-76 (2001)。