Studies on the distribution and diversification of S alleles in connectrion with self-and non-self recognition in Brassica in European countries

欧洲国家芸苔属植物S等位基因的分布及多样性与自我和非自我识别的关系研究

• 批准号: 07041123
• 负责人: HINATA Kokichi
• 金额: $ 1.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07041123/
• 关键词: BRASSICA; CAMPESTRIS; OLERACEA; NAPUS; NIGRA; self-incompatibility; S-ALLELE; GERMANY

The objective of this project research is to carry out a survey trip on wild Brassica campestris and B.oleracea in European countries and a collection of seeds of them on individual base to make analysis on the distribution of S-alleles, which are considered to participate in the recognition reaction of self-and nonself pollen/pistils.In order to investigate it in Germany we have visited Dr. Thomas Glades, institute of Plant Genetics and Crop Plant Research in Gatersleben, and made a trip to the place in where a wild B.campestris population was described to grow by kind help of him. However, the barley field was changed to pasture, according to very recent changes of agricultural situations, and we could not find any wild populations of this species in this country. Furthermore, it was suggested that this species is listed to be a possible species going to extinction.We have carried out a survey trip between Amsterdam and Utrecht in Netherland. Eight populations of B.napus was found on the creek sides and road sides. These populations, however, was considered to be escaped ones. B.nigra was also found near to some of these populations.At Folkestone, near to Dover, in England, we could find a good population of wild B.oleracea. They grow on the cliff against sea and the road on the base of cliff. More than hundred plants grew along the road for about 300 m. and we could obtain some seeds from them on individual base. Only few seeds were collected from each plant not to disturb the population structure of the population.It was revealed that wild B.campestris may grow at this moment near the Black Sea in Russia and Slovakia. It is not sure, but possibly grow as a weed in the field or rape cultivation in England or Denmark.This survey trip was partly successful in obtaining some seeds of B.oleracea, and we are going to make an analysis of S-alleles on this materials and to investigate the distribution and diversity of Salleles of wild populations.

本项目研究的目的是对欧洲国家的野生油菜和甘蓝进行调查，并在个体基础上收集其种子，分析s等位基因的分布，认为s等位基因参与了自性和非自性花粉/雌蕊的识别反应。为了在德国调查它，我们拜访了Thomas Glades博士，他是Gatersleben植物遗传学和作物植物研究所的一员，并在他的帮助下去了一个野生b.s campestris种群生长的地方。然而，根据最近农业情况的变化，大麦地变成了牧场，我们在这个国家找不到这种物种的野生种群。此外，该物种被列为可能灭绝的物种。我们在荷兰的阿姆斯特丹和乌得勒支之间进行了一次考察旅行。在溪边和路边发现了8个甘油三酯种群。然而，这些种群被认为是逃出来的。在其中一些种群附近也发现了黑桫椤。在英格兰多佛附近的福克斯顿，我们发现了大量的野生甘蓝。它们生长在临海的悬崖上，生长在悬崖脚下的道路上。沿路生长了大约300米的一百多株植物，我们可以在个别基地上从它们身上获得一些种子。为了不影响种群结构，每株只采集少量种子。据透露，在俄罗斯和斯洛伐克的黑海附近，野生的白僵菌可能正在生长。目前还不确定，但可能在英国或丹麦的田地里作为杂草或油菜种植。此次调查获得了部分甘蓝种子，我们将对这些种子进行s等位基因分析，并对甘蓝野生居群的s等位基因分布和多样性进行调查。