Studies on the distribution and diversification of S alleles in connectrion with self-and non-self recognition in Brassica in European countries

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The objective of this project research is to carry out a survey trip on wild Brassica campestris and B.oleracea in European countries and a collection of seeds of them on individual base to make analysis on the distribution of S-alleles, which are considered to participate in the recognition reaction of self-and nonself pollen/pistils.In order to investigate it in Germany we have visited Dr. Thomas Glades, institute of Plant Genetics and Crop Plant Research in Gatersleben, and made a trip to the place in where a wild B.campestris population was described to grow by kind help of him. However, the barley field was changed to pasture, according to very recent changes of agricultural situations, and we could not find any wild populations of this species in this country. Furthermore, it was suggested that this species is listed to be a possible species going to extinction.We have carried out a survey trip between Amsterdam and Utrecht in Netherland. Eight populations of B.napus was found on the creek sides and road sides. These populations, however, was considered to be escaped ones. B.nigra was also found near to some of these populations.At Folkestone, near to Dover, in England, we could find a good population of wild B.oleracea. They grow on the cliff against sea and the road on the base of cliff. More than hundred plants grew along the road for about 300 m. and we could obtain some seeds from them on individual base. Only few seeds were collected from each plant not to disturb the population structure of the population.It was revealed that wild B.campestris may grow at this moment near the Black Sea in Russia and Slovakia. It is not sure, but possibly grow as a weed in the field or rape cultivation in England or Denmark.This survey trip was partly successful in obtaining some seeds of B.oleracea, and we are going to make an analysis of S-alleles on this materials and to investigate the distribution and diversity of Salleles of wild populations.