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STUDIES ON THE EXPRESSION OF DOMINANCE/RECESSIVENESS IN THE SELF-INCOMPATIBILITY OF BRASSICEAE

十字花科植物自交不亲和性显性/隐性表现的研究

基本信息

批准号：
05454038
负责人：
HINATA Kokichi
金额：
$ 4.54万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Objective of this research is to carry out a survey on the dominant/recessive relationships of S-alleles in the self-incompatibility of Brassicaceae and to investigate the mechanism of the dominant/recessive relationships.Out of 300 possible combinations between 25 S-allele homozygotes of Brassica campestris, 275 F_1s were raised and their dominant/recessive relationships were surveyed in the two cases, ie heterozygotes in stigma or anther, separately. In the case of stigma heterozygotes, the S-alleles were classified into two groups ; codominant and dominant groups. In the case of anther heterozygotes, they were classified into three groups ; codominat, dominant and recessive groups. The dominant/recessive relationships of S-alleles were indepent between stigma and pollen. There were 14 combinations where the heterozygotes were compatible to both parental strains, whereas they were self-incompatible. These S-alleles are considered to be independent weakening genes.Several SLG,SRK genes were isolated from cDNA or genomic DNA libraries. In S9-homozygotes, the sequences through the promoter region and S-domain across about 1.7kb were completely identical between SLG and SRK.This identity should be concerned with the dominance relationship at the stigma side. In S29 homozygotes, SLG and SRB clones were isolated, and it was revealed that they are highly homologous to each other. Because of short period of this project research, we could not obtain whole the SLG and SRKs. However it is expected that the objective of this study will be attained in near future by the development of this line of study.

本研究的目的是对十字花科植物自交不亲和性中S等位基因的显性/隐性关系进行调查，并探讨显性/隐性关系的机制。在25个油菜S等位基因纯合子的300个可能的组合中，产生了275个F_1，并在两个案例中调查了其显性/隐性关系，即杂合子柱头或花药，分开。在柱头杂合子的情况下，S等位基因分为两组；共显性群体和显性群体。就花药杂合子而言，它们被分为三组；共显性、显性和隐性群体。 S等位基因的显性/隐性关系在柱头和花粉之间是独立的。在 14 种组合中，杂合子与两个亲本菌株均相容，但它们是自交不亲和的。这些S等位基因被认为是独立的弱化基因。从cDNA或基因组DNA文库中分离出几个SLG、SRK基因。在S9纯合子中，SLG和SRK之间从启动子区到S结构域的序列大约1.7kb是完全相同的。这种一致性应该与柱头侧的显性关系有关。在S29纯合子中，分离出SLG和SRB克隆，结果表明它们彼此高度同源。由于本项目研究时间较短，我们无法获得完整的SLG和SRK。然而，预计通过该研究方向的发展，本研究的目标将在不久的将来实现。