Rapid Bioassay with a Bio-Cell Tracer System (BCT)

本研究项目的目标分为两个部分：1.研究目标；将生物细胞示踪剂（BCT）的适用性扩展到各个领域；目的：探讨丝状funji标准方法制定的趋势。在1995.4-1998.3研究期间，获得了以下结果：(1)在临床领域，BCT测量精度显著提高（0.01 μ m/min），用于测量生长速度极低的致病性真菌。开发了一种高性能泵送系统来模拟药物在患者血液中的变化模式。最初为追踪酵母出芽而开发的模式识别程序也被应用于果脯形状的测量。(2)在生产过程中（药剂和农用化学品），使用BCT检测，发现许多中草药含有抗真菌化合物，而这些化合物是用灵敏度较低的常规方法检测不到的。主要从赤墨和雅坎中分离得到有效成分，并对其分子结构进行了分析。(3)在农业和环境测量中，对植物根系生长速率进行了敏感测量，并提出了将根系生长速率作为植物新鲜度指标的建议。测定了植物根系对金属离子和化感物质的反应，并提出了其作为水体和土壤中有毒化合物的生物指示物的价值。标准制定趋势：(1)NCCLS（国家临床实验室标准委员会）没有制定适用于丝状真菌的标准，也没有制定标准的计划。(2) AOAC未考虑丝状funji的标准方法。(3)我们决定尝试在BCT方法的基础上自行建立一种标准方法。(4)我们的努力也指向AOAC日本分部的建立，它可以提供一个程序，以确保包括BCT方法在内的分析方法的可靠性。

The objectives of this research project is divided to two items : 1. to extend the applicability of Bio-Cell Tracer (BCT) to various fields, and 2. to survey trends in the establishment of standard methods for filamentous funji. During the research period 1995.4-1998.3, the following results were obtained.1. Appfication to respective fields : (1) In crinical field, the measuring preciseness of BCT was markedly improved (0.01mu m/min) to measure pathogenic funji the growth rate of which was very low. A high performance pumping system was developed to simulate the changing patterns of a drug in patient blood. The pattern recognition program which was developed originally to trace yeast budding was also applied to the measurement of bolld shape. (2) In production processes (pharmaceutical agents and agricultural chemicals), using BCT,many Chinese herbal medicines were found to contain anti-fungal compounds which could not be detected by less sensitive conventional methods. Particularly from Chimo and Yakan, active components were isolated and their molecular structures were determined. (3) In agriculture and environmental measurements, the growth rate of a plant root was measured sensitively and its use as a freshness in dicator was proposed. The response of a plant root to metal ions and allelochemicals was measured and its wse as a bio-indicator of toxic compounds in waters and soil was also proposed.2. Trends in standard establishment : (1) NCCLS (National Committee for Clinical Laboratory Standards) did not establsh any standard applicable to filamentous funji nor had any plan to establish it. (2) AOAC did not consider any standard method for filamentous funji. (3) It was decided to try to establish a standard method based on BCT method by ourselves. (4) Our efforts were also deirected towards the foundation of AOAC Japan Section which could provide a procedure to assure the reliability of analytical methods including BCT method.