Association of HLA-DR and TAP genes with PSS in Koreans.-the basic trial

韩国人 HLA-DR 和 TAP 基因与 PSS 的关联——基础试验

• 批准号: 07044317
• 负责人: TAKEUCHI Fujio
• 金额: --
• 依托单位: Tokyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for International Scientific Research.
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044317/
• 关键词: PSS; Japanese; Korean; TAP1; TAP2; HLA-DR; PCR-PFLP; Immunogenetics

The contribution of genetic factors to PSS is still unclear. We started the co-work on TAP gene in Korean PSS with Seoul University in Korea. In this research period, the purpose of research is to establish the basical research conditions for co-work. For the purpose, we examined Japanese PSS to establish the research method and presented the research information to Korean researcher. TAP1 and TAP2 typing was carried out in 92 Japanese PSS patients and 95 normal subjects by PCR-RFLP method. HLA-DR typing and HLA DRB1**15, **16 and **08 genotyping were carried out by PCR method and PCR-SSCP method, respectively.For co-work, we established the universal database between Korean and Japanese. In PSS with diffuse scleroderma alleles frequencies of TAP1A and TAP2A were significantly increased (100%, p<0.005 ; 80.0%, p<0.001, respectively). In PSS with antitopisomerase I antibody (a-Scl-70) TAP1A and TAP2A alleles were also increased (93.2%, p=NS ; 63.6%, p<0.05). To the contrary, TAP1B allel … More e was significantly decreased in diffuse scleroderma (0%, p<0.005) and PSS with a-SCL-70 (4.5%, p<0.05). TAP2B was also decreased in diffuse scleroderma (12.5%, p<0.01). The examination of DR and DRB1 showed the significant increase in DRB1**1502 in diffuse scleroderma and a-Scl-70 positive PSS,as our previous study. Association analysis among TAP1A,TAP2A and DRB1**1502 indicated increases in TAP1A and TAP2A were not primary but reflect of an increase in HLA DRB1**1502 in Japanese PSS with diffuse scleroderma and PSS with a-Scl-70.The conclusion, however, was not concorded with Caucasians, so that informations from PSS in Korean were very important for clarify the genetic pathogenesis of PSS.In this study, Korean samples including Korean normal has been ccllected already, and several samples were tested, preliminary, in Seoul University. Additionally, clinical features has also been studied using universal protocol, above mentioned. TAP and DR genes of Korean PSS will be clarified after next research period. Less

遗传因素对 PSS 的影响尚不清楚。我们与韩国首尔大学开始了韩国PSS中TAP基因的合作研究。本研究期的研究目的是为合作研究建立基础研究条件。为此，我们考察了日本的PSS以建立研究方法，并将研究信息提供给韩国研究人员。采用PCR-RFLP方法对92例日本PSS患者和95例正常人进行TAP1和TAP2分型。分别采用PCR法和PCR-SSCP法进行HLA-DR分型和HLA DRB1**15、**16、**08基因分型。为了协同工作，我们建立了韩日通用数据库。在患有弥漫性硬皮病的 PSS 中，TAP1A 和 TAP2A 等位基因频率显着增加（分别为 100%，p<0.005；80.0%，p<0.001）。在使用抗拓扑异构酶 I 抗体 (a-Scl-70) 的 PSS 中，TAP1A 和 TAP2A 等位基因也增加 (93.2%，p=NS；63.6%，p<0.05)。相反，TAP1B 等位基因 e 在弥漫性硬皮病 (0%, p<0.005) 和 a-SCL-70 的 PSS (4.5%, p<0.05) 中显着降低。 TAP2B 在弥漫性硬皮病中也减少（12.5%，p<0.01）。 DR和DRB1检测显示弥漫性硬皮病和a-Scl-70阳性PSS中DRB1**1502显着升高，与我们前期的研究一致。 TAP1A、TAP2A和DRB1**1502之间的关联分析表明，TAP1A和TAP2A的增加不是主要的，而是反映了日本弥漫性硬皮病PSS和a-Scl-70 PSS中HLA DRB1**1502的增加。然而，该结论与白种人不一致，因此来自韩国PSS的信息对于阐明PSS的遗传发病机制非常重要。 这项研究已经收集了包括韩国正常人在内的韩国样本，并在首尔大学对多个样本进行了初步测试。此外，还使用上述通用方案研究了临床特征。韩国PSS的TAP和DR基因将在下一个研究周期后得到澄清。较少的

项目成果

Takeuchi Fujio et al.: "Association of TAP1 and TAP2 with RA in Japanese." (to be submitted).

Takeuchi Fujio 等人：“TAP1 和 TAP2 与日语中 RA 的关联。”

Pattanakitsakul S,Takeuchi Fujio et al.: "A novel new TAP2 gene RFLP observed in Japanese." Tissue Antigens. (in press). (1996)

Pattanakitsakul S、Takeuchi Fujio 等人：“在日语中观察到的一种新的 TAP2 基因 RFLP。”