Association of HLA-DR and TAP genes with PSS in Koreans.-the basic trial

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The contribution of genetic factors to PSS is still unclear. We started the co-work on TAP gene in Korean PSS with Seoul University in Korea. In this research period, the purpose of research is to establish the basical research conditions for co-work. For the purpose, we examined Japanese PSS to establish the research method and presented the research information to Korean researcher. TAP1 and TAP2 typing was carried out in 92 Japanese PSS patients and 95 normal subjects by PCR-RFLP method. HLA-DR typing and HLA DRB1**15, **16 and **08 genotyping were carried out by PCR method and PCR-SSCP method, respectively.For co-work, we established the universal database between Korean and Japanese. In PSS with diffuse scleroderma alleles frequencies of TAP1A and TAP2A were significantly increased (100%, p<0.005 ; 80.0%, p<0.001, respectively). In PSS with antitopisomerase I antibody (a-Scl-70) TAP1A and TAP2A alleles were also increased (93.2%, p=NS ; 63.6%, p<0.05). To the contrary, TAP1B allel … More e was significantly decreased in diffuse scleroderma (0%, p<0.005) and PSS with a-SCL-70 (4.5%, p<0.05). TAP2B was also decreased in diffuse scleroderma (12.5%, p<0.01). The examination of DR and DRB1 showed the significant increase in DRB1**1502 in diffuse scleroderma and a-Scl-70 positive PSS,as our previous study. Association analysis among TAP1A,TAP2A and DRB1**1502 indicated increases in TAP1A and TAP2A were not primary but reflect of an increase in HLA DRB1**1502 in Japanese PSS with diffuse scleroderma and PSS with a-Scl-70.The conclusion, however, was not concorded with Caucasians, so that informations from PSS in Korean were very important for clarify the genetic pathogenesis of PSS.In this study, Korean samples including Korean normal has been ccllected already, and several samples were tested, preliminary, in Seoul University. Additionally, clinical features has also been studied using universal protocol, above mentioned. TAP and DR genes of Korean PSS will be clarified after next research period. Less