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THE EGG-COAT MOLECULES INVOLVED IN FERTILIZATION AND DEVELOPMENTAL PROCESSES IN VARIOUS ANIMALS

参与各种动物受精和发育过程的蛋壳分子

基本信息

批准号：
07308051
负责人：
KATAGIRI Chiaki
金额：
$ 6.21万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07308051/
关键词：
VITELLINE ENVELOPE SPERM-VE BINDING CHORIION ZONA PELUCIDA SPERM RECEPTOR VITELLINE COAT LYSIN OVOPEROXIDASE GLYCOPROTEIN オビダクチン

项目摘要

(a) The carbohydrate moieties in 36-39KDa glycoproteins of Bufo japonicus vitelline coat are involved in binding with fertilizing sperm ; (b) cDNA encoding the major glycoprotein gp41 of Xenopus laevis vitelline envelope indicated that it is a homologue of mammalian ZP3 ; (c) The sperm receptor activity of porcine PZP3 resides in beta-Gal residues in beta-Gal residues of N-linked glycoside chains consisting fo tri-, and tetra-antennary, complex-type neutral chain ; (d) Biotinylated sea urchin sperm surface proteins were co-incubated with fucose sulfate glycoconjugates isolated from the jelly coat and were chromatographically separated, with the result that only two sperm proteins were coeluted with the pertinent jelly coat molecules ; (e) Employing the soft fertilization envelope of sea urchin eggs, a 70kDa ovoperoxidase (OPO) was isolated, and its primary structure elucidated on the basis of cloned cDNA indicated the substrate requirement of hatching enzyme ; (f) Comparison of the cDNA-based primary structures of the vitelline coat lysins from 7 species of Archeogastropods revealed that the species-specific sequences reside in 40-100 amino acid residues in the central portion of each molecule ; (g) The jelly coat of the starfish eggs was differentially irradiated by high-energy electrons and the acrosome-reaction-inducing (ARI) activity was monitored, with the result that the minimum size exhibiting ARI activity was of 14kDa molecules containing a plenty amount of sugar residues ; (h) Cloning of cDNAs for the egg envelope proteins of Oryzias latipes revealed that a low molecular-weight subunit (ZI-3) is homologous with mammalian ZP3, and its gene is expressed exclusively in the liver but not in the ovary.

(A)东亚钳蝎卵黄衣36-39 KDa糖蛋白中的碳水化合物部分参与与受精精子的结合，(B)编码非洲爪哇卵黄膜主要糖蛋白gp41的cDNA表明它是哺乳动物ZP3的同源物，(C)猪PZP3的精子受体活性存在于N-连接的三触角和四触角复合型中性链的β-Gal残基中；(D)将生物素化的海胆精子表面蛋白与从凝胶层中分离的岩藻糖硫酸酯共孵育，并进行层析分离，结果只有两个精子蛋白与相应的凝胶层分子一起洗脱；(E)利用海胆卵的软受精包膜，分离到一个70 kDa的卵巢过氧化物酶(OPO)，其一级结构在克隆的cDNA的基础上阐明了孵化酶的底物需求；(F)比较了7种古足类动物卵黄皮裂解酶的一级结构，发现物种特有的序列位于每个分子中央部分的40-100个氨基酸残基；(G)用高能电子对海星卵进行差异辐射，并对其顶体反应诱导活性进行了监测，结果表明，具有顶体反应诱导活性的最小分子大小为14 kDa，其中含有大量的糖残基；(H)克隆了宽叶稻卵被蛋白的cDNAs，发现其低分子量亚基(Zi-3)与哺乳动物ZP3同源，其基因只在肝脏中表达，而在卵巢中不表达。