ESTABLISHMENT OF STRAINS OF XENOPUS LAEVIS AND THEIR APPLICATION TO DEVELOPMENTAL AND GENETIC STUDIES

非洲爪蟾品系的建立及其在发育和遗传研究中的应用

• 批准号: 60440100
• 负责人: KATAGIRI Chiaki
• 金额: $ 15.17万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60440100/
• 关键词: XENOPUS LAEVIS; STRAIN; MAJOR HISTOCOMPATIBILITY COMPLEX (MHC); FOURTH COPLEMENT COMPONENT (C4); GERM CELLS; LYMPHOCYTES; リンパ球; 免疫学的寛容性; アフリカツメガエル; 純系; 組織適合遺伝子複合体(MHC); 胸腺; 免疫寛容性; T細胞抗原; 血球幹細胞; 始原生殖細胞; 補体第4成分; 胸腺リンパ球抗原

1. Two large colonies of strains were established in the anuran amphibian, Xenopus laevis: J-line (MHC haplotype j/j)produced by repeated inbreedings, and K-line (k/k) produced by gynogenetic procedure. The (JxK)F_1 frogs suffered from a typical GVH reaction by inoculation of parental J splenocytes; 2. The fourth complement component (C4) was isolated from plasma, and isoelectric focusing analyses of this 202KD protein in combination with MLR- and skin graft analyses indicated a strong association of C4 IEF-pattern to the MHC-haplotype segregation pattern, 3. A monoclonal antibody (XT-1) sspecific to T-lymphocytes was established. The XT-1, a 120KD membrane glycoprotein, was expressed strongly in J-line frogs but relatively weakly in K- or outbred frog, and served as an excellent marker of functional T-cells; 4. The amount of germ plasm (GP) in cleaving embryos had a good correlation with the number of primordial germ cells (PGC) differentiated in larvae. The number of PGCs in J-line l … More arvae was less variable and higher than in outbred larvae, suggesting the genetic control over the amount of GP; 5. Chimeras produced by grafting cytogenetically labeled tissue cells at tailbud stage were analyzed for the embryonic source of blood cells. It resulted that all types of blood cells in early larvae are derived from the ventral blood island (VBI) whereas those in late larvae and adults are from the dorsal lateral plate mesoderm in addition to VBI; 6. Early-thymectomized, triploid J-frogs (j/j/j) were grafted with <gamma>-irradiated thymuses from diploid (JxK)F_1 frogs (j/k). Immune responsiveness restored in the host frogs was associated with the specific tolerance to the k haplotype, due to a deletion of clones reactive to thymus donors. The J (j/j)line frogs were rendered tolerant to K skin donors by receiving skin grafts at early laraval stage from (j/k) adults. MLR- and lymphocyte transfer analyses indicated that this type of tolerance was maintained by the activities of specific suppressor cells. Less

1. 在非洲爪蟾（Xenopus laevis）中建立了两个大的菌落：通过重复近交产生的j系（MHC单倍型j/j）和通过雌体发生产生的k系（k/k）。（JxK）F_1代蛙接种亲代J脾细胞后发生典型的GVH反应；2. 第四个补体成分（C4）从血浆中分离出来，该202KD蛋白的等电聚焦分析结合MLR和皮肤移植分析表明C4 ief模式与mhc单倍型分离模式有很强的相关性。建立t淋巴细胞特异性单克隆抗体（XT-1）。XT-1是一种120KD的膜糖蛋白，在j系青蛙中表达强烈，而在K系或远交系青蛙中表达相对较弱，是功能性t细胞的良好标记；4. 卵裂胚胚质（GP）数量与幼虫分化的原始生殖细胞（PGC）数量有良好的相关性。j系1 ~ 2龄幼虫的遗传变异较小，且高于远交种幼虫，表明遗传控制了GP的数量；5. 对细胞遗传学标记的组织细胞在尾芽期移植产生的嵌合体进行了血细胞胚胎来源分析。结果表明，早期幼虫的各类血细胞均来源于腹侧血岛（VBI），而后期幼虫和成虫的各类血细胞除来源于腹侧血岛外，还来源于背外侧板中胚层；6. 早期胸腺切除的三倍体j蛙（j/j/j）移植了二倍体（JxK）F_1 （j/k）的< γ >辐照胸腺。由于对胸腺供体有反应的克隆的删除，宿主蛙的免疫应答性恢复与对k单倍型的特异性耐受性有关。J （J / J）系蛙在幼年早期接受（J / K）成年蛙的皮肤移植，使其对K皮肤供体产生耐受性。MLR-和淋巴细胞转移分析表明，这种耐受性是由特异性抑制细胞的活性维持的。少

项目成果

片桐 千明.栃内 新: "講座『免疫科学』第4巻 第6章『両生類と爬虫類の免疫系』" 岩波書店, PP.137-166 (1985)

Chiaki Katagiri. Arata Tochinouchi：“讲座‘免疫学’第 4 卷第 6 章‘两栖动物和爬行动物的免疫系统’”岩波书店，PP.137-166 (1985)

Nakamura,T.,H.Kawahara and Ch.Katagiri: "Rapid production of a histocompatible colony of Xenopus laevis by gynogenetic procedure." Zool. Sci.2. 71-79 (1985)

Nakamura,T.,H.Kawahara 和 Ch.Katagiri：“通过雌核发育程序快速产生组织相容性非洲爪蟾群体。”

Nagata,S.: "Development of T lymphocytes in Xenopus laevis: appearance of the antigen recogized by an anti-thymocyte mouse monoclonal antibody." Develop. Biol.114. 389-394 (1986)

Nagata,S.：“非洲爪蟾 T 淋巴细胞的发育：抗胸腺细胞小鼠单克隆抗体识别抗原的出现。”

Nagata,S.: Dev.Biol.114. 389-394 (1986)

Nagata，S.：Dev.Biol.114。

Eur.J.Immunol.15-6. (1985)

Eur.J.Immunol.15-6。