Study on Development and Clinical Application of Hybrid Type Artificial Bone Marrow Using Bioceramics-Development of Hybrid-type Immune Organ-

以生物陶瓷为研究对象，建立了形态学、分子生物学、生物力学及实验进化研究相结合的三边研究方法。基于这些研究方法，通过生物力学刺激，在生物陶瓷和钛制成的生物室周围诱导细胞分化。作者已经报道了用烧结羟基磷灰石（HA）人工骨髓腔植入哺乳动物（狗和猴子）成功诱导造血巢。本文采用三边研究方法，报道了以软骨鱼（chondrichthyes）和盲鳗（cyclostomata）为研究对象，在肌肉中人工诱导骨髓造血。这两种鱼类没有带骨髓的骨内骨架，只有placoid中的HA骨，代表了原型脊椎动物的系统发育阶段。在鲨鱼和环口肌中成功地获得了羟基磷灰石腔内类骨组织诱导的造血场。通过本实验，可以通过生物力学结合透明质酸或钛的材料效应来理解类骨组织诱导造血的作用。对于实验造血场，选择肌肉，骨替代，使用烧结多孔管HA人工骨和带电钛。1994年10月获第32届日本人工器官学会独创性奖。为了研究骨髓造血的进化机制，我们进行了实验进化研究。人工骨髓腔是由烧结羟基磷灰石和钛制成的，使用原型脊椎动物，即软骨鱼和环口动物植入，它们的内部没有羟基磷灰石骨架，只有软骨。在原型脊椎动物肌肉内植入人工透明质酸骨后，可观察到造血与类骨组织发生结合的现象。

A trilateral research method integrating morphology, molecular biology, and biomechanics as well as experimental evolutionary research are developed using bioceramics. Based on these research methods, cell differentiations are induced around biochamber made of bioceramics and titanium by biomechanical stimuli. The authors already reported the successful inducement of hemopoietic nests with sintered hydroxyapatite (HA) artificial bone marrow chambers implanted in mammals (dogs and monkeys.) In this paper, through the trilateral research method, artificial inducement of bone marrow hemopoiesis in muscles is reported using dochi-zame (chondrichthyes) and hagfish (cyclostomata), which have no inner skeletons of bone with marrow but HA bone only in placoids, and represent the phylogenical stage of archetype vertebrates. Successful inducement of hematopoietic fields with osteoid tissue in hydroxyapatite chambers was obtained in muscles of shark and cyclostomata. Through this experiment inducement of hemopoiesis with osteoid tissue can be understood by biomechanics conjugated with the material effect of HA or titanium.For an experimental hemopoietic field, muscles were selected, and for bone substitution, sintered porous tubular HA artificial bone and titanium with electricity are used.This work was awarded the originality prize of the 32nd Japanese Society of Artificial Organs in October, 1994. To study evolutionary mechanisms of bone marrow hemopoiesis, experimental evolutionary study are carried out. Artificial bone marrow chambers made of sintered HA and titanium are implanted using archetype vertebrate, i.e., chondrichthyes and cyclostomata, which have no inner skeleton of hydroxyapatite but cartilage. Hemopoiesis conjugated with osteoid tissue genesis could be observed around artificial HA skeleton implanted into muscles of archetype vertebrates.