Development of Hybrid Type Artificial Bone Marrow Made of Hydroxyapatite Chamber

羟基磷灰石室混合型人工骨髓的研制

• 批准号: 03557107
• 负责人: NISHIHARA Katsunari
• 金额: $ 3.97万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557107/
• 关键词: Hydroxyapatite; Phylogeny; Mesenchymal Tissue; Artificial Bone Marrow Chamber; High Pressure Gas Technique; Hemopoietic Microenvironment; Spleen; Reticulo-endothelial System; 微小造血環境; 人工骨髄chamber; 骨髄造血; ヒドロキシアパタイト; 高圧低温法; 巨核球; 血小板; 人工骨髄; ジル

Artificial inducement of bone marrow tissue with hemopoietic inductive microenvironment (HIM) in sintered hydroxyapatite (HA) chamber in vivo was carried out. This research is important to disclose the mechanisms of hemopoiesis. From a phylogenic standpoint, it is known that HIM definitely existed in the organ nearest the digestive tract, i. e., spleen, in an early stage of evolution. In the second revolution of vertebrates, the internal skeleton changed into bone tissue from cartilage. From this fact of phylogenesis, we should be able to induce hemopoietic nests in a HA chamber in place of bone, if we can find optimal structural conditions. For a preliminary study, the following experiments were carried out to observe the component effect of sintered apatite upon mesenchymal tissue : 1) apatite granules were applied to a dental pulp chamber clinically : 2) Sintered apatite plates were placed into rib bone marrow : and 3) A section of femur with marrow was removed, after which tubular- … More type apatite artificial bone was implanted and fixed with an A-O plate. After a fixed period, specimens were made and then histopathologically examined.Thereafter, we tried to induce artificially a hematopoietic field in muscles using sintered porous tubular hydroxyapatite and a new type HA plate made by a high-pressure gas technique. Then, we developed an artificial bone marrow chamber made of sintered HA,which was connected to the iliac artery of a dog. Distinct resorption of hydroxyapatite porous plates was observed in rib bone marrow without marked tissue reaction. Not only in the pore sites of tubular HA artificial bone but at the surface of the new type HA plate implanted in the dorsal muscles, differentiation of bone marrow cell clusters of the hematopoietic field could be observed.The implanted artificial bone marrow chamber was also examined histopathologically. A reticuroendothelial system was observed in the HA bone marrow chamber without bone and bone marrow tissue. In muscle tissue bone marrow tissue with bone formation in the HA chamber could be observed Less

在体内的烧结型羟基磷灰石(HA)小室中，用造血诱导微环境(HIM)对骨髓组织进行人工诱导。这一研究对于揭示其造血机制具有重要意义。从系统发育的观点来看，在进化的早期，它确实存在于离消化道最近的器官中，即脾。在脊椎动物的第二次革命中，内部骨骼由软骨转变为骨组织。根据这一系统发育的事实，如果我们能找到最佳的结构条件，我们应该能够在HA室中诱导造血巢而不是骨。为了初步观察烧结型磷灰石对间充质组织的成分效应，进行了以下实验：1)临床上将磷灰石颗粒应用于牙髓室：2)将烧结型磷灰石板置入肋骨骨髓中：3)取出一段有骨髓的股骨，然后进行Tube-…。植入更多类型的磷灰石人工骨，用A-O钢板固定。在固定时间后，制作标本并进行组织病理学检查，然后尝试使用烧结多孔管状羟基磷灰石和高压气体技术制作的新型HA平板在肌肉中人工诱导造血场。然后，我们研制了一种由烧结HA制成的人工骨髓室，将其连接到一只狗的髂动脉上。肋骨骨髓内可见明显的羟基磷灰石多孔板吸收，未见明显组织反应。不仅在管状羟基磷灰石人工骨的孔洞部位，而且在植入背部肌肉的新型羟基磷灰石人工骨板表面，都可以观察到造血场的骨髓细胞群的分化。HA骨髓室内可见网状内皮系统，未见骨和骨髓组织。在肌肉组织中，HA腔内有骨形成的骨髓组织较少见

项目成果

K.Nishihara,: "Clinical Application of Hydroxyapatite Artificial Root of Fibrous Tissue Attachment Type" Bioceramics. 4. 223-230 (1991)

K.Nishihara，：“纤维组织附着型羟基磷灰石人工根的临床应用”生物陶瓷。

K.Nishihara: "Comparative Histopathological Studies on Stoichiometric and Nonstoichiometric Sintered Hydroxyapatite" Transaction of the 8th Symposium on Apatite. 8. 14 (1992)

K.Nishihara：“化学计量和非化学计量烧结羟基磷灰石的比较组织病理学研究”第八届磷灰石研讨会论文集。

K.Nishihara: "Comparative Studies on Periodontal Tissues Around New Type Artificial Roots Made of ZrO_2,Ti.,and Hydroxyapatite" Phosphorus Research Bulletin. 1. 179-184 (1991)

K.Nishihara：“ZrO_2、Ti.和羟基磷灰石制成的新型人工牙根周围牙周组织的比较研究”磷研究通报。

K.NISHIHARA,T.KOBAYASHI,T.TANGE, K.HIROTA, and Y.HIRAYAMA: "Experimental Study on Artificial Bone Marrow" Apatite. 12-13 (1992)

K.NISHIHARA、T.KOBAYASHI、T.TANGE、K.HIROTA 和 Y.HIRAYAMA：“人工骨髓的实验研究”磷灰石。

K.Nishihara,: "On Autogenous Tooth Transplantation with Application of Sintered HAP Granules" Phosphorus Research Bulletin. 1. 197-202 (1991)

K.Nishihara，：“关于应用烧结 HAP 颗粒的自体牙齿移植”磷研究通报。