Development of Hybrid Type Artificial Bone Marrow Made of Hydroxyapatite Chamber

在烧结羟基磷灰石（HA）小室中，利用造血诱导微环境（HIM）对骨髓组织进行了体内人工诱导。本研究对揭示造血机制具有重要意义。从生殖的观点来看，HIM肯定存在于离消化道最近的器官中，即。例如，脾脏，在进化的早期阶段。在脊椎动物的第二次革命中，内部骨骼从软骨变成了骨组织。从造血的事实来看，如果我们能找到最佳的结构条件，我们应该能够在HA室中诱导造血巢而不是骨。为初步研究烧结磷灰石对间充质组织的成分效应，进行了以下实验：1）临床上将磷灰石颗粒应用于牙髓室; 2）将烧结磷灰石板置于肋骨骨髓中; 3）切除股骨骨髓段，然后进行管状骨移植。 ...更多信息 型磷灰石人工骨植入，用A-O钢板固定。固定时间后，制作标本，进行组织病理学检查，然后尝试用烧结多孔管状羟基磷灰石和高压气体技术制成的新型HA板在肌肉内人工诱导造血场。然后，我们开发了一种由烧结HA制成的人工骨髓室，将其连接到狗的髂动脉。在肋骨骨髓中观察到羟基磷灰石多孔板的明显吸收，无明显组织反应。不仅在管状HA人工骨的孔隙处，而且在植入背肌的新型HA板表面，均可观察到造血区骨髓细胞团的分化，并对植入的人工骨髓腔进行了组织病理学检查。在HA骨髓腔中观察到网状内皮系统，无骨和骨髓组织。在肌肉组织中，可以观察到HA腔室中有骨形成的骨髓组织较少

Artificial inducement of bone marrow tissue with hemopoietic inductive microenvironment (HIM) in sintered hydroxyapatite (HA) chamber in vivo was carried out. This research is important to disclose the mechanisms of hemopoiesis. From a phylogenic standpoint, it is known that HIM definitely existed in the organ nearest the digestive tract, i. e., spleen, in an early stage of evolution. In the second revolution of vertebrates, the internal skeleton changed into bone tissue from cartilage. From this fact of phylogenesis, we should be able to induce hemopoietic nests in a HA chamber in place of bone, if we can find optimal structural conditions. For a preliminary study, the following experiments were carried out to observe the component effect of sintered apatite upon mesenchymal tissue : 1) apatite granules were applied to a dental pulp chamber clinically : 2) Sintered apatite plates were placed into rib bone marrow : and 3) A section of femur with marrow was removed, after which tubular- … More type apatite artificial bone was implanted and fixed with an A-O plate. After a fixed period, specimens were made and then histopathologically examined.Thereafter, we tried to induce artificially a hematopoietic field in muscles using sintered porous tubular hydroxyapatite and a new type HA plate made by a high-pressure gas technique. Then, we developed an artificial bone marrow chamber made of sintered HA,which was connected to the iliac artery of a dog. Distinct resorption of hydroxyapatite porous plates was observed in rib bone marrow without marked tissue reaction. Not only in the pore sites of tubular HA artificial bone but at the surface of the new type HA plate implanted in the dorsal muscles, differentiation of bone marrow cell clusters of the hematopoietic field could be observed.The implanted artificial bone marrow chamber was also examined histopathologically. A reticuroendothelial system was observed in the HA bone marrow chamber without bone and bone marrow tissue. In muscle tissue bone marrow tissue with bone formation in the HA chamber could be observed Less