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Construction of Processes for Production and Separation of Pigments Originated from Plant

植物源颜料生产与分离工艺的构建

基本信息

批准号：
07405057
负责人：
TONE Setsuji
金额：
$ 4.99万
依托单位：
OSAKA UNIVERCITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07405057/
关键词：
PIGMENTS OF PLANTS BETANIN ANTHRAQUINONE PLAT HAIRY ROOTS PRODUCT EXTRACTION MADDER RED BEET ENZYMATIC REACTION 溶媒抽出

项目摘要

Plants have playd an important role in supplying human being with many physiologically active substances since ancient times. Moreover, plants have been important as a raw materials for beverages, medicines, pigments and food additives on an industrial scale. The success in commercial use of plant cell-derived products requires knowledge of the principles of plant cell genetics, physiology, metabolic regulation, and bioprocess engineering. Many problems remain to be solved in culture systems which facilitate high yields of both biomass and the desired metabolites.This project dealt with construction of culture systems suitable to the production of plant-derived pigments by using plant hairy roots. To resolve some key problems related with engineering aspects in hairy root cultures and to develop production process, red beet and madder hairy roots were examined.1. For the improvement of culture conditions, the strategy for enhancement of productivity of desired metabolite was proposed. … More The effect of medium modification on pigment productivity was investigated in the culture of red beet hairy roots and phosphate was identified as an important constituent which stimulated pigment formation. In the culture of madder hairy roots, carbon and nitrogen sources were important factors and the productivity of pigment was drastically enhanced in the culture using ammonium-free medium containing fructose as a carbon source. Moreover, concerning the kinetics of hairy root proliferation, the model based upon the linear extension and lateral branching of the growing points at root tip made it possible to simulate and characterize the growth of various hairy roots.2. For the development of the culture operation for the effective production of pigment by red beet and madder hairy roots, repeated cultures associated with operations of growth and pigment release were performed. The pigments from madder hairy roots were defined as the alizarin, lucidin and their glycosides. To achieve the higher yield for the alizarin which is target product, the enzymatic reaction accompanied with extraction by organic solvent was performed. beta-gulcosidase was suitable to selective hydrolysis of alizarin glycoside and hexane was chosen for the extraction of alizarin. Less

植物自古以来就在为人类提供多种生理活性物质方面发挥着重要作用。此外，植物在工业规模上作为饮料、药品、色素和食品添加剂的原料也很重要。植物细胞衍生产品的商业应用的成功需要植物细胞遗传学、生理学、代谢调节和生物过程工程原理的知识。本课题的目的是构建适合于利用植物毛状根生产植物色素的培养体系。为解决毛状根培养中的一些工程问题，开发毛状根生产工艺，对红甜菜和茜草根进行了研究.为改善培养条件，提出了提高目标代谢产物产量的策略。 ...更多信息以红甜菜毛状根为材料，研究了培养基改良对色素产生的影响，确定磷酸盐是促进色素形成的重要成分。在茜草毛状根培养中，碳源和氮源是重要因素，以果糖为碳源的无铵培养基能显著提高毛状根色素的产量。此外，在毛状根增殖动力学方面，基于根尖生长点的线性延伸和侧向分枝的模型能够模拟和表征各种毛状根的生长.为了开发红甜菜和茜草根状根有效生产色素的培养操作，进行了与生长和色素释放操作相关的重复培养。茜草毛状根色素主要为茜素、光泽素及其苷类。为了提高目的产物茜素的收率，采用酶促反应结合有机溶剂萃取的方法。β-葡萄糖苷酶适合于茜素苷的选择性水解，正己烷适合于茜素苷的提取。少