JOINT STUDY ON BIOCHEMICAL PROCESSES WITH INTEGRATED UNITS

与集成装置的生化过程联合研究

• 批准号: 07044152
• 负责人: TONE Setsuji
• 金额: $ 5.95万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044152/
• 关键词: BIOPRODUCTION; BIOSEPARATION; BIOREACTOR; REVERSE MICELLES; LIPOSOMES; TISSUE CULTURE; AGITATION CULTURE WITH LOW SHEAR STRESS; OPTIMIZATION OF BIOPROCESS; 生体触媒反応; 自己増殖型リポソーム; マイクロバイオリアクター; 動植物細胞培養; 低剪断力撹拌培養; 低剪断撹拌培養

The development of effective bioproduction and purification processes of the new biopolymers, such as medically useful peptides, antibiotics, engineered proteins and various enzymes, has been urgently needed to meet the increasing demands in various industries.The major objective of this project was to establish effective and advanced units of production and separation through the exchange of different ideas and experts in the institutions, Dept. of Chem.Sci.Eng., Osaka University, Institute for Polymer and Chem.Eng.Dept. (TCL), ETH.This project exploited topical functional materials, membranes, liposomes, aqueous two-phase systems etc. It also included the construction of sophisticated bioreactors based on the analyzes of flow dynamics, design and control of reactors, and optimization of the processes.The obtained findings from this international research project contributed to the basic and technological progress of bioseparation strategies and novel bioreactor systems to cultivate microbes and animal or plant cells, and also to minimize environmental loading. The other important outcome was to contribute to both university academics and industrial engineers by surveying up-to-date information on educational and research systems in both countries.

本项目的主要目的是通过各研究所、工程系、实验室、科研机构化学科学与工程学院，大坂大学高分子化学研究所(TCL)该项目开发了局部功能材料，膜，脂质体，双水相系统等，还包括基于流动动力学分析的复杂生物反应器的构建，反应器的设计和控制，从这个国际研究项目中获得的结果有助于生物分离策略和新型生物反应器系统的基础和技术进步，以培养微生物和动物或植物细胞，以及最小化环境负荷。另一个重要成果是通过调查两国教育和研究系统的最新信息，为大学学者和工业工程师做出贡献。

项目成果

V.Dorovska-Taran: "A^1H=NMR Method for Investigating the Phospholipase D Catalyzed Hydrolysis of Phosphatidicholine in Liposomes" Anal.Biochem.240. 37-47 (1996)

V.Dorovska-Taran：“研究脂质体中磷脂酶 D 催化水解磷脂酰胆碱的 A^1H=NMR 方法”Anal.Biochem.240。

P.L.Luisi: "Self-Reproduction of Micelles and Vesicles: Method for the Mechanisms ofLife from the Perspective of Compartmented Chemistry" Adv.Chem.Phys.42. 425-438 (1996)

P.L.Luisi：“胶束和囊泡的自我复制：从区室化学角度研究生命机制的方法”Adv.Chem.Phys.42。

K.Morigaki: "Autopoietic Self Reproduction of Chiral Fatty Acid Vesicles" J.Am.Chem.Soc.119. 292-301 (1996)

K.Morigaki：“手性脂肪酸囊泡的自生成自我复制”J.Am.Chem.Soc.119。

R.Kuboi: "Refolding of Carbonic Anhydrase Assisted by 1-Palmitoy 1-2-oleoyl-sn-glycero-3- phosphocholine Liposomes" Biotechnol.Prog.13. 828-836 (1997)

R.Kuboi：“1-棕榈酰 1-2-油酰-sn-甘油-3-磷酸胆碱脂质体辅助碳酸酐酶的重折叠”Biotechnol.Prog.13。

H.Ota: "Stabilization of an Enzyme by Using Hydrophobic Ligands" J.Chem.Eng.Japan. 31. 118-122 (1998)

H.Ota：“使用疏水性配体稳定酶”J.Chem.Eng.Japan。