Staging of brain and liver function in brain death by non-invasive MR method utilizing stable isotope.

利用稳定同位素通过非侵入性 MR 方法对脑死亡患者的脑和肝功能进行分期。

• 批准号: 07408028
• 负责人: INUBUSHI Toshiro
• 金额: $ 22.34万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07408028/
• 关键词: ^<13>C,; NMR-tracer,; glucose,; non-invasive,; brain,; liver,; brain death,; staging; 肝代謝機能; 多量子遷移; デカップリング; 生体NMR

1.Highly sensitive in vivo ^<13>C NMR method has been developed by the integration of hetero-nuclear multiple resonance capability with the basis of clinical imager. New RF system and field gradient control system was incorporated into an existing MR imager. The original MR imaging system was not modified in the hardware.2.High sensitivity of ^<13>C NMR detection has been achieved by employing hetero-nuclear multiple quantum coherence between ^1H and ^<13>C in combination with gradient selection pulses. The signal intensity in the spin system passing through multiple quantum coherence was found to be affected by the rapid relaxaing behavior in the multiple quantum spin system. Therefore, the highest sensitivity for the outcome was obtained with optimizing the shape and duration of the selection gradient pulses.3.Injected glucose labeled by ^<13>C into living organs was traced non-invasively by ^1H-detected ^<13>C NMR method. Glucose reached to the highest level in 30 minutes and decreased rapidly, however, glutamine/glutamate derived by the metabolic reaction of the glucose reached to the highest level in 1 hour and stayd in a this high level for approximately two more hours.4.Metabolic stagin in liver was performed non-invasively by ^<13>P and ^<13>C NMR spectroscopy and found that the accumulation of glucose as glycogen and metabolic reaction of alanine to such as glutamine, glutamate, and glucose was related to the level of ATP and also to gluconeogenesis and glycogenesis.5.Further improvement will be expected with easier operation of the developed MR system as well as a development of automatically analyzing software of the metabolic response which will help to accurately judge the state of the brain dead patients.

1.将<13>异核多重共振技术与临床成像技术相结合，发展了高灵敏度的活体~ 13 C NMR方法。新的射频系统和场梯度控制系统被整合到现有的MR成像仪中。2.<13>利用^1H和^ C之间的异核多量子相干性<13>，结合梯度选择脉冲，实现了高灵敏度的~ 1C NMR探测。研究发现，多量子自旋系统中的快速弛豫行为会影响通过多量子相干的自旋系统中的信号强度。因此，优化选择梯度脉冲的形状和持续时间可以获得最高的灵敏度。3.<13>采用~ 1H检测的~ 1C NMR方法对活体器官内注射的~ 1C标记葡萄糖进行无创追踪<13>。葡萄糖在30分钟内达到最高水平，并迅速下降，然而，由葡萄糖代谢反应产生的谷氨酰胺/谷氨酸在1小时内达到最高水平，并在此高水平下保持约两小时。4.通过~ 1 <13>P和~ 1C <13>NMR光谱法非侵入性地进行肝脏中的代谢阶段，发现葡萄糖作为糖原的积累和丙氨酸代谢反应，如谷氨酰胺、谷氨酸和葡萄糖与ATP水平有关，与脑组织的新生和糖原生成有关。5.随着MR系统操作的简化以及代谢反应自动分析软件的开发，将有助于准确判断脑死亡患者的状态，该系统还有待进一步完善。

项目成果

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Takahashi,K.: "Effects of halothane and isoflurane on phosphoenergeticastate of the liver during hemorrhagic shock in rats." Anesth.Analg.85. 347-352 (1997)

Takahashi,K.：“氟烷和异氟烷对大鼠失血性休克期间肝脏磷酸能状态的影响。”

Morikawa,S.: "Dissociation between lactate accumulation and acidosis in middle cerebral artery-occluded rats assessed by ^<31>P and ^1H metabolic..." Magnetic Resonance Imaging. 14. 1197-1204 (1996)

Morikawa，S.：“通过^ 31 P和^ 1 H代谢评估大脑中动脉闭塞大鼠中乳酸积累和酸中毒之间的分离......”磁共振成像。

Morikawa S,Inubushi T,Kito K,et al: "Longterm observationof in vivo ^<31>P NMR spectra in carbon tetrachloride intoxicated rabbit liver using implanted wireless surface coil." NMR Biomed. 8. 38 (1995)

Morikawa S、Inubushi T、Kito K 等人：“使用植入的无线表面线圈对四氯化碳中毒兔肝脏中的体内 31 P NMR 谱进行长期观察。”