Non-invasive analyses of brain metabolic function in dementia

痴呆症脑代谢功能的无创分析

• 批准号: 10358017
• 负责人: INUBUSHI Toshiro
• 金额: $ 18.94万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10358017/
• 关键词: ^<13>C MR spectroscopy; MR-tracer method; glucose; non-invasive mesurement; brain; near infra red; spectrophotometory; blood oxygen level; 化学シフト画像; 脳循環代謝; 代謝画像; 脳機能; エコー・プラナーCSI; C-13MR; C-13標識; 局所勾配磁場コイル; 高速撮像; グルコース; 近赤外分光器; NIR画像

1. A local gradient coil with water cooling jacket and a fitted RF coil were newly incorporated into imager system. With this gradient/RF coil assembly it is possible to obtain high-resolution MR images, for example, an MR image with 128 x 128 pixels, in echo-planar (EPI) type fast scanning for functional MRI.Further, a fast scanning EPI approach was incorporated into newly developed highly sensitive in vivo ^<13>C MR spectroscopy method, which was developed in our laboratory by the integration of hetero-nuclear multiple resonance capability with the basis of clinical imager.2. Injection of glucose enriched with ^<13>C into living organs was traced non-invasively by ^1H-detected ^<13>C in vivo MR spectroscopy. The level of the glucose reached to the highest mark in 30 minutes after the njection and followed rapid decrease with 1 hour. In contrast to this, the metabolites glutamine and glutamate produced by the metabolic reaction of the glucose reached to the highest level in 1 hour aft … More er the injection and stayed at this high level for approximately two more hours. In this period, it was able to obtain the distribution of these metabolites as well as the precursor glucose with chemical shift imaging.3. A visible spectrophotometer has been developed in order to precisely measure blood oxygen level in a model living animal. Sensitivity enhancement for blood-oxygen level detection was taken care by observing a Soret-band wavelengths for oxy and deoxy hemoglobin and their isosbestic point for the absorbance curves, whose intensities calculate the concentration of each component of hemoglobin and as long as the total concentration of hemoglobin in the brain. Another feature of the photometer is that this instrument has independent two detectors, which can simultaneously measure right and left hemisphere in rat brain.4. A near infra red spectrometer was also employed to non-invasively monitor oxygen level in blood. The spectrophotometer used for this purpose has 750 and 830nm light sources and 13 channel detectors, which was developed in Dr. Chance's laboratory at University of Pennsylvania. With this instrument oxygenation of hemoglobin and total concentration of hemoglobin were evaluated for patient brain and the maps of these components were also constructed in approximately 30 seconds. Less

1.在成像系统中新加入了带水冷夹套的局部梯度线圈和安装的射频线圈。利用这种梯度/射频线圈组件，可以获得高分辨率的MR图像，例如，128 x 128像素的MR图像，在回声平面(EPI)型快速扫描功能磁共振成像中。此外，快速扫描EPI方法被结合到新开发的高灵敏在体磁共振波谱方法中，该方法是我们实验室通过将异核多重共振能力与临床图像相结合而开发的。~1H检测~(13)&gt；C活体磁共振波谱无创示踪富含~(13)&gt；C的葡萄糖进入活体器官。血糖在注射后30min达高峰，1h后迅速下降。与此相反，葡萄糖代谢反应产生的代谢物谷氨酰胺和谷氨酸在…后1小时达到最高水平更多的呃注射，并在这个高水平停留了大约两个多小时。在此期间，可以通过化学位移成像获得这些代谢物的分布以及前体葡萄糖的分布。为了精确测量活体动物模型的血氧水平，研制了一种可见分光光度计。为了提高检测血氧水平的灵敏度，通过观察氧合和脱氧血红蛋白的Soret波段波长及其吸光度曲线的等色点，其强度计算出血红蛋白的各组分的浓度，以及大脑中的血红蛋白总浓度。光度计的另一个特点是，该仪器有两个独立的探测器，可以同时测量大鼠大脑的左右半球。近红外光谱仪也被用来非侵入性地监测血液中的氧气水平。用于这一目的的分光光度计有750 nm和830 nm光源以及13个通道探测器，这是由宾夕法尼亚大学Chance博士的实验室开发的。用这台仪器评估了患者大脑的血红蛋白氧合和总血红蛋白浓度，并在大约30秒内构建了这些成分的地图。较少

项目成果

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