Device development to measure cellular activity in brain

开发测量大脑细胞活动的设备

• 批准号: 09044287
• 负责人: INUBUSHI Toshiro
• 金额: $ 3.07万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044287/
• 关键词: ^<13>C; NMR-tracer; glucose; non-invasive; brain; near infra red; optical imaging; blood oxygen level; NIR画像; 酸素動機; 酸素運搬; 一酸化窒素

1. Fast scanning echo planar imaging (EPI) approach was incorporated into newly developed highly sensitive in vivo ^<13>C NMR method, which was developed in our laboratory by the integration of hetero-nuclear multiple resonance capability with the basis of clinical imager.2. Injected glucose labeled by ^<13>C into living organs was traced non-invasively by ^1H-detected ^<13>C NMR method. Glucose reached to the highest level in and decreased rapidly, however, glutamine/glutamate derived by the metabolic reaction of the glucose reached to the highest level in 1 hour and stayed in a this high level for approximately two more hours.3. This fast imaging technique made it possible to obtain ^<13>C-labled chemical compounds in rat brain within 30 minute period. These were l-^<13>C-glucose as a precursor and its metabolites, 2-, 3-, and 4- glutamine/glutamate. Therefore, it is feasible to follow the metabolic change during brain ischemia in model animals.4. Near infra red spectroscopy was employed to monitor non-invasively oxygen level in blood. For this a spectrophotometer having 750 and 830nm light sources and 13 channel detector was developed in Dr.Chance's laboratory. With this instrument deoxygenation and blood volume were traced during brain ischemia in rat and their maps were also constructed in approximately 30 seconds.5. A half NO-liganded hemoglobin was elucidated to be in typical T-structure by spectroscopic and oxygen binding studies. Therefore, this hemoglobin may be utilized as an effective oxygen carrier to deceased part in living tissue. In addition, the paramagnetism of this hemoglobin may be used as a contrast reagent in MR imaging, which can delineate blood vessels in living animas.

1.将快速扫描回波平面成像（EPI）方法与<13>本实验室在临床成像基础上结合异核多共振能力开发的高灵敏度活体~ 1C NMR方法相结合.用<13>~ 1H检测~ 1C NMR方法对活体器官注射~ 1C标记葡萄糖进行无创示踪<13>。葡萄糖达到最高水平并迅速下降，然而，由葡萄糖代谢反应产生的谷氨酰胺/谷氨酸在1小时内达到最高水平并在该高水平下保持约两小时。这种快速成像技术使得有可能<13>在30分钟内获得大鼠大脑中的13 C标记的化合物。这些是<13>作为前体的1-β C-葡萄糖及其代谢物2-、3-和4-谷氨酰胺/谷氨酸盐。因此，在模型动物脑缺血期间跟踪代谢变化是可行的.采用近红外光谱法无创监测血液中的氧水平。为此，Chance博士的实验室开发了具有750和830 nm光源和13通道检测器的分光光度计。利用该仪器在30秒左右的时间内对大鼠脑缺血时的脱氧和血容量进行了追踪，并绘制了相应的图谱.通过光谱和氧结合研究，确定了一种半NO配体血红蛋白具有典型的T型结构。因此，这种血红蛋白可以作为有效的氧载体，在活体组织中到达死亡部位。此外，这种血红蛋白的顺磁性可以用作MR成像中的对比剂，其可以描绘活体动物的血管。

项目成果

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Morikawa S、Inubushi T 等人：“通过体内 13 C 和 31 P NMR 光谱评估大鼠肝脏中糖异生和磷酸能学之间的关系”。

Ishii H. et al.: "Evaluation of focal cerebral ischemical in rats by nagnetic resonance imaging and immunhistochemical analyses." Journal of Cerebral Blood Flowand Metabolism. 18. 931-934 (1999)

Ishii H. 等人：“通过磁共振成像和免疫组织化学分析评估大鼠局灶性脑缺血。”

Takahashi K,Shigemori S,et al.: "Effects of halothane and isoflurane on phosphoenergetic state of the liver during hemorrhagic shock in rats : an in vivo ^<31>P nuclear magnetic resonance spectroscopic study." Anesth Analg. 85. 347-52 (1997)

Takahashi K、Shigemori S 等人：“氟烷和异氟烷对大鼠失血性休克期间肝脏磷酸能状态的影响：一项体内 ^<31>P 核磁共振波谱研究。”

Bruley, D.F.: "Feasibility study of a single-and multiple-source near infrared phase modulation device for characterizing" Biomed. Instrum. Technol.31. 373-386 (1997)

Bruley, D.F.：“用于表征生物医学的单源和多源近红外相位调制装置的可行性研究”。

Okada M,Mitsunami K et al.: "Influence of aging or left ventricular hypertrophy on the human heart : contents of phosphorus metabolites measured by ^<31>P MRS." Magn Reson.Med.38. 772-782 (1998)

Okada M，Mitsunami K等人：“衰老或左心室肥大对人类心脏的影响：通过^ 31 P MRS测量的磷代谢物的含量”。