Studies of molecular genetics on the basis of protein and nucleic acid preserved in fossil materials.

基于化石材料中保存的蛋白质和核酸的分子遗传学研究。

基本信息

批准号：
07454126
负责人：
CHIBA Satoshi
金额：
$ 5.06万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07454126/
关键词：
Fossils,DNA Protein Molecular Biology Nucleotide sequences PCR technique Mollusca PCR 昆虫化石タンパク質化石DNA SDS‐PAGE

项目摘要

Condition of preservation of biomolecules such as protein and nucleic acid were examined, and potential of estimating phylongenetic relationship of fossil organisms by nsing these biomolecules was evaluated. Intracrystalline protein was extraced from four modern species (Patinopecten yessoensis, Umbonium giganteum, Sunetta menstrualis and Crassostrea gigas) and one fossil species (Patenipecten tokyoensis) of mollusca. Patenipecten tokyoensis were occurred from Pliocene deposits of Kakegawa fromation. Twenty-one species of protein were detected by electrophoretic analysis with SDS-PAGE.Protein molecules obtained from Patenipecten yessosensis and Patenipecten tokyoensis were restricted at the position of methionine. Restriction patterns of these molecules are coincident between modern species Patenipecten yessoensis and fossil species Patenipecten tokyoensis. Since these species are considered to be closely related, overall similarity of restriction patterns of protein between these spec … More ies suggest that protein have been preserved in the fossil materials.Phylogenetic relationship among the molluscan species estimated on the basis of the variations in the species of protein possessed by these molluscan species well corresponded to the phylogenetic relationships on the basis of nuclear DNA sequence (lrRNA). This suggests that the preserved protein in the fossils are useful to assess phylogenetic relationships. Nuclear and mitochondrial DNA were extracted from fossil insects (Donaciinae, Carabidae, Hydrophilinae) correceted from the Pleistocene and Holocene peat. These DNAs were amplified via PCR,and 300bp of nuclear 18SrRNA and mitochondrial 16SrRNA were sequenced. Sequences of the fossil species of Hydrophilinae were the closest to those of living species of Hydrophilinae among sequences obtained from Genbank. This suggests that DNAs of fossil Hydrophilinae species are amplified successfully and sequenced. This also suggests that DNA was preserved in the fossil insects occurred from peat. Less

检查了生物分子（例如蛋白质和核酸）的保存条件，并评估了通过NSS NSS NSS估算这些生物分子的估计化石生物的潜能。从四种现代物种（patinopecten Yessoensis，giganteum，Sunetta menstrualis和Crassostrea gigas）中提取丙烯酸内蛋白和一种Mollusca的化石（Patenectecten tokyoensis）。 patececten tokyoensis是从世界上卡盖川的上新世沉积物中出现的。通过用SDS-PAGE进行电泳分析检测到21种蛋白质。从patenectecten Yessensis获得的蛋白质分子和Patenececten tokyoensis在甲基氨酸位置受到限制。这些分子的限制模式在现代物种patenecten yessoensis和化石物种之间是一致的。由于这些物种被认为是密切相关的，因此这些规格之间蛋白质的限制模式的总体相似性……更多的IS表明，蛋白质已保存在化石材料中。根据这些Molluscan物种所具有的蛋白质种类的变化，与这些Molluscan物种在这些蛋白质物种中的变化估算的晶状体发育关系很好地对应于核DNA基础上的核生理关系（LRRNA）。这表明化石中保留的蛋白质可用于评估系统发育关系。从更新世和全新世果皮中释放的化石绝缘（Donacinae，carabidae，hydophilinae）中提取核和线粒体DNA。通过PCR扩增这些DNA，并测序了300bp的核18sRRNA和线粒体16sRNA。在从GenBank获得的序列中，水热化石种类的序列与亲水性生物的生物最接近。表明化石氢磷脂物种的DNA被成功地放大并进行了测序。这也表明DNA保存在果皮发生的化石昆虫中。较少的