Studies of molecular genetics on the basis of protein and nucleic acid preserved in fossil materials.
基于化石材料中保存的蛋白质和核酸的分子遗传学研究。
基本信息
- 批准号:07454126
- 负责人:
- 金额:$ 5.06万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Condition of preservation of biomolecules such as protein and nucleic acid were examined, and potential of estimating phylongenetic relationship of fossil organisms by nsing these biomolecules was evaluated. Intracrystalline protein was extraced from four modern species (Patinopecten yessoensis, Umbonium giganteum, Sunetta menstrualis and Crassostrea gigas) and one fossil species (Patenipecten tokyoensis) of mollusca. Patenipecten tokyoensis were occurred from Pliocene deposits of Kakegawa fromation. Twenty-one species of protein were detected by electrophoretic analysis with SDS-PAGE.Protein molecules obtained from Patenipecten yessosensis and Patenipecten tokyoensis were restricted at the position of methionine. Restriction patterns of these molecules are coincident between modern species Patenipecten yessoensis and fossil species Patenipecten tokyoensis. Since these species are considered to be closely related, overall similarity of restriction patterns of protein between these spec … More ies suggest that protein have been preserved in the fossil materials.Phylogenetic relationship among the molluscan species estimated on the basis of the variations in the species of protein possessed by these molluscan species well corresponded to the phylogenetic relationships on the basis of nuclear DNA sequence (lrRNA). This suggests that the preserved protein in the fossils are useful to assess phylogenetic relationships. Nuclear and mitochondrial DNA were extracted from fossil insects (Donaciinae, Carabidae, Hydrophilinae) correceted from the Pleistocene and Holocene peat. These DNAs were amplified via PCR,and 300bp of nuclear 18SrRNA and mitochondrial 16SrRNA were sequenced. Sequences of the fossil species of Hydrophilinae were the closest to those of living species of Hydrophilinae among sequences obtained from Genbank. This suggests that DNAs of fossil Hydrophilinae species are amplified successfully and sequenced. This also suggests that DNA was preserved in the fossil insects occurred from peat. Less
考察了蛋白质、核酸等生物分子的保存条件,评价了利用这些生物分子评价化石生物系统发育关系的潜力。从软体动物的4个现代种(Patinopecten yessoensis、Umbonium Giganteum、Sunetta mentruis和Crassostrea gias)和1个化石种(Patenipecten Tokyoensis)中提取了晶内蛋白。Patenipecten tokyoensis产于上新世Kakegawa组沉积。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对21种蛋白质进行了分析,得到的蛋白质分子均限制在蛋氨酸的位置。这些分子的限制模式在现代物种Yessoens和化石物种Patenipecten tokyoensis之间是一致的。由于这些物种被认为是密切相关的,所以这些特定…之间蛋白质限制性内切酶图谱的总体相似性这些软体动物物种之间的系统发育关系由这些软体动物物种所拥有的蛋白质种类的差异来估计,与基于核DNA序列(LrRNA)的系统发育关系很好地对应。这表明化石中保存下来的蛋白质对评估系统发育关系是有用的。从更新世和全新世泥炭中提取了化石昆虫(石龙亚科、步甲亚科、水虫亚科)的核和线粒体DNA。通过聚合酶链式反应(PCR)扩增出这些DNA,并对其进行了测序。在Genbank获得的序列中,亲水亚科化石种的序列与活的亲水亚科物种的序列最接近。这表明成功地扩增出了嗜水虫化石的DNA并对其进行了测序。这也表明,从泥炭中产生的昆虫化石中保存了DNA。较少
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kapoor,M: "The chloroplastchil gene of the green alga Chlorella Vilgaris C-27 contains a self-spiking group lintion" Mol.Gen.Genet.250. 655-664 (1996)
Kapoor,M:“绿藻 Chlorella Vilgaris C-27 的叶绿体基因包含一个自尖峰基团”Mol.Gen.Genet.250。
- DOI:
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- 影响因子:0
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Sunouchi, N., F.Sasaki, S.Chiba and M.Kawata.: "Morphological stasis and phylogentic relationships in Tadpole shrimps (Triops)." Biological Journal of the Linnean Society. 61. 439-458 (1997)
Sunouchi, N.、F.Sasaki、S.Chiba 和 M.Kawata.:“蝌蚪虾(Triops)的形态停滞和系统发育关系。”
- DOI:
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- 影响因子:0
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Sunouchi N.: "Morphological stasis and phylogenetic velatiorship in tadpole shimps." Biol.Jour.Linn.Soc.61. 439-457 (1997)
Sunouchi N.:“蝌蚪小虾的形态停滞和系统发育变化。”
- DOI:
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- 影响因子:0
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Chiba, S.: "Novel color polymorphisms in hybrid zone of Mandarina (Pulmonata)" Biological Journal of the Linnean Society. 61. 369-389 (1997)
Chiba, S.:“柑橘(Pulmonata)杂交区的新型颜色多态性”林奈学会生物学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sunouchi N.Sasaki F.Chiba, S.Kawata M.: "Morphological stasis and phylogenetic relationships in Tadpole shrimps." Biological Jouranal of the Linnean Society. 61. 439-458 (1997)
Sunouchi N.Sasaki F.Chiba,S.Kawata M.:“蝌蚪虾的形态停滞和系统发育关系。”
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- 影响因子:0
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{{ truncateString('CHIBA Satoshi', 18)}}的其他基金
Conservation palaeontology: protecting future biodiversity by past information
保护古生物学:通过过去的信息保护未来的生物多样性
- 批准号:
24340128 - 财政年份:2012
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
STUDY FOR LONG-TERM CHARACTERISTICS OF WATER AND SEDIMENT QUALITY OF ISE BAY BY A PELAGIC - MARINE SEDIMENT COUPLED ECOSYSTEM MODEL
中上层-海洋沉积物耦合生态系统模型研究伊势湾水沙质量长期特征
- 批准号:
23510016 - 财政年份:2011
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Detection of human mediated evolution by fossil records
通过化石记录检测人类介导的进化
- 批准号:
21340151 - 财政年份:2009
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
evelopment of theoretical method to calculate neutron-induced fission cross sections
计算中子诱发裂变截面的理论方法的发展
- 批准号:
18560805 - 财政年份:2006
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MECHANISMS OF ADAPTIVE DIVERGENCES
适应性分歧的机制
- 批准号:
18204048 - 财政年份:2006
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Evolution in frontier environments
前沿环境的演变
- 批准号:
15340174 - 财政年份:2003
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
COLONISATION OF THE LAND: A CASE STUDY IN GASTROPODA
土地殖民化:腹足类动物案例研究
- 批准号:
12440140 - 财政年份:2000
- 资助金额:
$ 5.06万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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