Establishment of the methodology to determine nutritional status in higher plants using molecular biological approaches

建立利用分子生物学方法确定高等植物营养状况的方法

基本信息

  • 批准号:
    07456037
  • 负责人:
  • 金额:
    $ 4.42万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

Three major experiments were conducted under this projects.1) A fragment cDNA corresponding to a gene of Arabidopsis regulated by boron nutrition was isolated by the method called differential display. The methods utilizes the PCR procedure to compare population of two or more mRNA populations.2) Arabidopsis metallothionein gene, which we previously isolated in the lab, was found to respond to the elevated levels of Cu in roots but not in leaf tissues. Anaerobic stresses were also found to be involved in the regulation processes.3) The gene encoding the beta subunit of beta conglycinin, a major seed storage protein of soybean, was found to respond to sulfur and nitrogen nutrition. A DNA fragment in the promoter responsible for the regulation was identified.
本研究主要进行了三个方面的实验:1)利用差异显示技术分离了拟南芥硼营养调控基因的cDNA片段。该方法利用PCR程序比较两个或多个mRNA群体的群体。2)拟南芥金属硫蛋白基因,我们以前在实验室分离,被发现响应于根中的铜水平升高,但不响应于叶组织。3)大豆种子贮藏蛋白β伴大豆球蛋白(beta conglycinin)β亚基基因对硫、氮营养具有响应性。鉴定了负责调控的启动子中的DNA片段。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Wei-Ming Shi et al.: "Effect of CaCN_2 treatment of Seed of Inubie on Synthesis of Protein and RNA during germination" Soil Science Plant Nutrition. 42・3. 443-449 (1996)
Wei-Ming Shi 等:“CaCN_2 处理 Inubie 种子对发芽过程中蛋白质和 RNA 合成的影响”土壤科学植物营养 42・3 443-449(1996)。
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    0
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M.YOKOTA-Hrai.et al.: "Effect of Nitrogen Nutrition on the Exprossion of a Soybean Seed Storage Protein Gene in Trausgeric Arobidpodsis thaliana" Sulfur Nutr.and Assimilation,ed Cram J.,SPB Academic Pross.(in press). (1997)
M.YOKOTA-Hrai.et al.:“氮营养对 Trausgeric Arobidpodsis thaliana 中大豆种子储存蛋白基因表达的影响”Sulfur Nutr.and Assimilation,Cram J. 编,SPB 学术期刊(出版中)。
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    0
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M.Awazuhara et al.: "Changes in Metallothion air.gene expression in Response to Sulfur Nutrition in C&d Mutant." Sulfur Nutr.and Assimilation,ed Gram J.,SPB Academic Press.(in press). (1997)
M.Awazuhara 等人:“金属硫离子空气基因表达的变化对 C 中硫营养的反应
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    0
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藤原徹: "イオウ栄養に応じた遺伝子発現制御" RADIOISOTOPES. 46. 59-60 (1997)
Toru Fujiwara:“硫营养反应中基因表达的调节”RADIOISOTOPES 46. 59-60 (1997)。
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  • 影响因子:
    0
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  • 通讯作者:
T.Fujiwara et al.: "Ragulation of Soybean Seed Strage Protein Gene-A Model for Sulfur Nutrition-Regulated Gene Expression" Sulfur Nutr.and Assimilation,ed Gram J.,SPB Academic Pross.(in press). (1997)
T.Fujiwara 等人:“大豆种子基质蛋白基因的调节 - 硫营养调节基因表达的模型”硫营养与同化,Gram J. 编,SPB 学术期刊(出版中)。
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CHINO Mitsuo其他文献

CHINO Mitsuo的其他文献

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{{ truncateString('CHINO Mitsuo', 18)}}的其他基金

"Man-Earth System : A scientific approach to realize human society in harmony with the Earth"-Molecular Breeding of Plants Resistant to Nutrient Stress-Isolation of Plant Genes Responsible for Stress Resistance-
《人地系统:实现人类社会与地球和谐相处的科学途径》-抗营养胁迫植物分子育种-植物抗逆基因分离-
  • 批准号:
    05278105
  • 财政年份:
    1993
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Nutrient behavior and function of root exudate in rhizosphere
根际根系分泌物的营养行为和功能
  • 批准号:
    04806010
  • 财政年份:
    1992
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Isolation of a gene for metallothionein-like protein of higherplant
高等植物金属硫蛋白样蛋白基因的分离
  • 批准号:
    02454058
  • 财政年份:
    1990
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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