Research on Nitrification : Isolation and Suppression of Nitrifing Bacteria

硝化作用研究：硝化细菌的分离和抑制

• 批准号: 07456041
• 负责人: SATO Yukiharu
• 金额: $ 3.26万
• 依托单位: Tamagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456041/
• 关键词: Nitrification; Nitrification inhibitors; Nitrosomonas europaea ATCC25978; Nitrobacter agilis ATCC14123; 1,3,5-Triazine; アンモニアモノオキシゲナーゼ; 1,3,5-トリアジン系化合物; Nitrosomonas europaea ATCC 25978; 硝化作用抑制剤; アンモニア酸化細菌; 亜硝酸酸化細菌

(1)In spite that it is difficult to prepare 1,3,5-triazines having different substituents, 2,4-bis(trichloromethy1)-6-trifluoromethy 1-1,3,5-triazine and 2-methy 1-4-trichloromethy 1-6-trifluoromethy 1-1,3,5-triazine were synthesized by the condensation reaction between corresponding imidoy 1-amidines and acid anhydrides. Other many of 1,3,5-triazines were synthesized using trimerization reaction and co-trimerization reactions.(2)1,3,5-Triazines having fluorine substituents showed weak nitrification inhibitory activity but 2-amino-4-tribromomethy 1-6-trichloromethy 1-1,3,5-triazine very strongly suppressed the growth of Nitrosomonas europaea ATCC25978 (pI_<50>=7.12). It was 65 times stronger than that of authentic nitrification inhibitor, MAST (pI_<50>=5.31).(3)We made a new ammonia bicarbonate (ABC) medium for the culture of N.europaea ATCC25978. In this medium N.europaea grew 3 times faster than in Lewis & Pramer medium.(4)Attempt to isolate new nitrifying bacteria using ABC medium from soil is not sucessful yet.(5)In the bioassay using N.europaea ATCC25978, N.sp.TK794, Nitorobacter agilis ATCC14123, and N.sp TH21., synthesized 1,3,5-triazines inhibited the growth of genus Nitorosomonas.(6)In the cell free level assay of O_2-consumption by N.europaea using ammonium sulfate as substrates, the target enzyme of synthesized 1,3,5-triazines is found to be an ammonia-monooxygenase.

(1)In尽管难以制备具有不同取代基的1，3，5-三嗪，但通过相应的亚氨基脒与酸酐之间的缩合反应，合成了2，4-双（三氯甲基）-6-三氟甲基-1，3，5-三嗪和2-甲基-4-三氯甲基-6-三氟甲基-1，3，5-三嗪。其他许多1，3，5-三嗪是通过三聚反应和共三聚反应合成的。(2)1含氟取代基的1，3，5-三嗪类化合物对硝化反应的抑制作用较弱，而2-氨基-4-三溴甲基-6-三氯甲基-1，3，5-三嗪类化合物对欧洲亚硝化单胞菌ATCC 25978的生长有很强的抑制作用（pI <50>=7.12）。该菌株对硝化作用的抑制能力是标准硝化抑制剂MAST（pI =5.31）的65倍<50>。(3)We制备了一种新的用于欧洲烟草ATCC 25978培养的碳酸氢铵（ABC）培养基。在此培养基上，欧洲猪笼草的生长速度比在刘易斯&amp;普拉默培养基上快3倍。（4）用ABC培养基从土壤中分离新硝化细菌的尝试尚不成功。(5)In使用欧洲拟单胞菌ATCC 25978、N. sp. TK 794、Nitoraviagilis ATCC 14123和N.sp TH 21.的生物测定，合成的1，3，5-三嗪类化合物对硝化单胞菌的生长有抑制作用。(6)In以硫酸铵为底物的无细胞水平氧耗测定表明，合成1，3，5-三嗪的靶酶是氨单加氧酶。

项目成果

Takahashi I.: "Mode of Action and QSAR Studies of Nitrification Inhibitors : Effect of Trichloromethyl-1,3,5-Triazines on Ammonia-oxidizing Bacteria" Journal of Pesticide Science. 22. 27-32 (1997)

Takahashi I.：“硝化抑制剂的作用方式和 QSAR 研究：三氯甲基-1,3,5-三嗪对氨氧化细菌的影响”农药科学杂志。

Ohki S., Kasahara Y., Murakami M., Miyamoto Y., Tokuyama T., Vonk J.W., Sato Y., and Wakabayashi K.: ""Effects of Halomethyl-1,3,5-triazines on Nitrification"" Journal of Pesticide Science. 22. 95-101 (1997)

Ohki S.、Kasahara Y.、Murakami M.、Miyamoto Y.、Tokuyama T.、Vonk J.W.、Sato Y. 和 Wakabayashi K.：“卤甲基-1,3,5-三嗪对硝化作用的影响”期刊

I.Takahashi: "Mode of Action and QSAR Studies of Nitrification Inhibitors : Effect of Trichloromethyl-1,3,5-triazines on Ammonium-oxidizing Bacteria" Journal of Pesticide Science. 22. 27-32 (1997)

I.Takahashi：“硝化抑制剂的作用方式和 QSAR 研究：三氯甲基-1,3,5-三嗪对铵氧化细菌的影响”农药科学杂志。

Ohki S.: "Effects of Halomethyl-1,3,5-triazines on Nitrification" Journal of Pesticide Science. 22. 95-101 (1997)

Ohki S.：“卤甲基-1,3,5-三嗪对硝化作用的影响”农药科学杂志。

Manabu Murakami: "A Possible Target Site of 1,3,5-Triazine Nitrification Inhibitors" Journal of Pesticide Science. 20. 471-477 (1995)

Manabu Murakami：“1,3,5-三嗪硝化抑制剂的可能目标位点”农药科学杂志。