Origin of tetrodotoxin and toxification mechanism in tetrodotoxin-bearing pufferfishes and Japanese newt
河豚毒素的来源及河豚毒素和日本蝾螈的中毒机制
基本信息
- 批准号:07456091
- 负责人:
- 金额:$ 4.99万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
High molecular weight substances (TTX-binding substances) which had ability to bind tetrodotoxin (TTX) were proved to be contained in the blood plasma of puffer fishes. Most of TTX in the plasma existed in a state of binding with the subsances. The TTX-binding substances had ability to bind not only TTX but also saxitoxin (STX). Small amount of STX bound with the substances existed naturally in the plasma. All the plasma of puffer fishes examined showed TTX-binding ability. In them the plasma of finepatterned puffer, Takifugu poecilonotus, showed the strongest activity, its one mililiter plasma could bind 23 mouse units of TTX.The TTX-binding substances unbound TTX under acidic condition and bound under neutral condition reversibly. The TTX-binding substances bound STX rather than TTX but did not bind goniotoxins. The TTX-binding substances were examined for neutralizing effects against the lethal activity of TTX or STX.When the mixture of the TTX-binding substances and TTX or STX was injected i.p. into mice, the lethal activity of TTX or STX was significantly reduced.Japanese newt is one of TTX-bearing animals. Specimens were collected from several spots at different vertical levels in Sanriku town, Iwate prefecture. The specimens were assayd for toxicity by mouse assay. A wide individual variation of toxicity was recognized among the specimens caught from a given spot. The local variation in toxicity was also large. There was a tendency that the toxicity was higher in specimens collected at high elevation than at low elevation. Toxicity of the newts collected from rice fields near seashore was increased 100 days after transportation them from the rice fields to a marsh near the top of a mountain.
河豚鱼血浆中含有与河豚毒素(TTX)结合的高分子量物质(TTX结合物质)。血浆中的TTX大部分以与物质结合的状态存在。TTX结合物质不仅能结合TTX,而且还能结合石杉毒素(STX)。血浆中自然存在少量与物质结合的STX。所有受检河豚鱼的血浆均显示TTX结合能力。其中,河豚的血浆活性最强,其1毫升血浆可与23个小鼠单位的河豚毒素结合。河豚毒素结合物质在酸性条件下解离TTX,在中性条件下可逆结合。TTX结合物质结合STX而不是TTX,但不结合弓形虫毒素。观察TTX结合物质对TTX或STX致死活性的中和作用。在小鼠体内,TTX或STX的致死活性显著降低。日本鳗是携带TTX的动物之一。标本从岩手县三里库镇不同垂直水平的几个地点采集。用小鼠试验法对标本进行毒性测定。在特定地点捕获的样本中,毒性存在很大的个体差异。毒性的局部差异也很大。在高海拔地区采集的样品有毒性高于低海拔地区的趋势。从海滨附近的稻田收集的蝾螈,在从稻田运送到靠近山顶的沼泽100天后,其毒性增加。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kunio Yamamori: "Biological activities of marine toxins (Japanese)" Aquabiology. 18 (4). 279-283 (1996)
Kunio Yamamori:“海洋毒素的生物活性(日语)”水生生物学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
山森邦夫: "魚貝毒の生物活性" 海洋と生物. 18(4). 279-283 (1996)
Kunio Yamamori:“鱼类和贝类毒素的生物活性”海洋和生物科学 18(4) (1996)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kunio Yamamori: "Proteins involved in accumulation of tetrodotoxin in a pufferfish (Japanese)" Nippon Suisan Gakkaishi. 62 (6). 948-949 (1996)
Kunio Yamamori:“参与河豚毒素积累的蛋白质(日语)”Nippon Suisan Gakkaishi。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
山森郁夫: "魚貝毒の生物活性" 海洋と生物. 18(4). 279-283 (1996)
Ikuo Yamamori:“鱼类和贝类毒素的生物活性”海洋与生物学 18(4) (1996)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
山森郁夫: "フグ毒の蓄積に関与するタンパク質" Nippon Suisan Gakkaishi. 62(6). 948-949 (1996)
Ikuo Yamamori:“参与河豚毒素积累的蛋白质”Nippon Suisan Gakkaishi 62(6) 948-949 (1996)。
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- 影响因子:0
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YAMAMORI Kunio其他文献
YAMAMORI Kunio的其他文献
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{{ truncateString('YAMAMORI Kunio', 18)}}的其他基金
Origin of tetrodotoxin and toxification mechanism in juvenile puffer fishes
河鲀幼鱼河豚毒素的来源及中毒机制
- 批准号:
14560159 - 财政年份:2002
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Origin of tetrodotoxin and toxification mechanism in tetrodotoxin-bearing animals
河豚毒素的来源及河豚毒素动物的中毒机制
- 批准号:
09460090 - 财政年份:1997
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Comparison of the effects of adaptation on olfaction and taste in fish.
比较适应对鱼类嗅觉和味觉的影响。
- 批准号:
05660219 - 财政年份:1993
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Origin and metabolism of tetrodotoxin in pufferfishes and newts
河豚毒素在河豚和蝾螈中的来源和代谢
- 批准号:
02660203 - 财政年份:1990
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Gustatory responses to tetrodotoxin and saxitoxin in fishes
鱼类对河豚毒素和石房蛤毒素的味觉反应
- 批准号:
61560221 - 财政年份:1986
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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