Origin and metabolism of tetrodotoxin in pufferfishes and newts

河豚毒素在河豚和蝾螈中的来源和代谢

• 批准号: 02660203
• 负责人: YAMAMORI Kunio
• 金额: $ 1.34万
• 依托单位: School of Fisheries Sciences, Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660203/
• 关键词: pufferfish; newt; shore crab; Tetrodotoxin; origin; metabolism; detoxication; 解毒

Supposing that the origin of tetrodotoxin(TTX)in pufferfishes and newts was exogenous, some studies on the origin and metabolism of TTX in these animals were conducted. 1. Toxicity of 27 species of marine invertebrates collected at Sanriku coast was examined, but no toxic animals were found. 2. To investigate the behavior of TTX in non-toxic cultured puffer fish, TTX was administered intravenously via an intravenous cannula. TTX concentration in the blood was progressively decreased in an hour after the administration, suggesting that TTX might be accumulated in certain organs. 3. A half of TTX administered intraperitoneally in non-toxic cultured pufferfish, was accumulated in liver. 4. Toxicity of a few species of invertebrates collected in a marsh inhabited by newts was examined, but no toxic animals were found. 5. There were remarkable individual and local variations of toxicity in newts. Comparison of the toxicity of newts collected from rice fields at different altitude suggested that the altitude has effects on the toxicity of newts. 6. Toxicity of newts changes with their growth. Adults are very toxic. Larvae are not so toxic. Toxicity of immature newts are not known. 7. The shore crab Hemigrapsus sanguineus was found to have anti-TTX substances with a high molecular weight in their body fluid. Preinjection of the body fluid into a mouse was effective to some extent in protecting from TTX challenge.

在河豚毒素(TTX)来源于外源性的假设下，对河豚毒素在这些动物体内的来源和代谢进行了研究。1.对三里库海域采集的27种海洋无脊椎动物进行了毒性检测，未发现有毒动物。2.为了研究河豚毒素在无毒养殖河豚鱼体内的行为，通过静脉插管给药。给药后1小时内血中TTX浓度呈进行性下降，提示TTX可能在某些器官内蓄积。3.在无毒养殖河豚体内，有一半的TTX在肝脏中蓄积。4.对采集于沼泽中的几种无脊椎动物进行了毒性检测，未发现有毒动物。5.蝾螈的毒性存在显著的个体和局部差异。通过对不同海拔条件下采集的水稻田蝾螈毒性的比较，表明海拔高度对其毒性有一定的影响。6.蝾螈的毒性随其生长发育而变化。成年人的毒性很大。幼虫没有那么大的毒性。未成熟蝾螈的毒性尚不清楚。7.中华绒螯蟹体液中含有高分子量的抗TTX物质。小鼠体内预先注射体液对TTX攻击有一定的保护作用。

项目成果

T.Matsui: "Toxification of cultured puffer fish by the administration of tetrodotoxin producing bacteria." Nippon Suisan Gakkaishi. 56. 705 (1990)

T.Matsui：“通过施用产生河豚毒素的细菌来使养殖河豚中毒。”

K. Yamamori, S. Yamaguchi, E. Maehara, and T. Matsui: "Tolerance of shore crabs to tetrodotoxin and saxitoxin and antagonistic effect of their body fluid against the toxins." Nippon Suisan Gakkaishi.

K. Yamamori、S. Yamaguchi、E. Maehara 和 T. Matsui：“岸蟹对河豚毒素和石房蛤毒素的耐受性及其体液对毒素的拮抗作用”。

T. Matsui, S. Taketsugu, H. Sato, K. Yamamori, K. Kodama, A. Ishii, H. Hirose and C. Shimizu: "Toxification of cultured puffer fish by the administration of tetrodotoxin producing bacteria." Nippon Suisan Gakkaishi. 56-4. 705 (1990)

T. Matsui、S. Taketsugu、H. Sato、K. Yamamori、K. Kodama、A. Ishii、H. Hirose 和 C. Shimizu：“通过施用产生河豚毒素的细菌对养殖河豚鱼进行毒性”。

T.Matsui: "Toxification of cultuted puffer fish by the administration of tetrodotoxin producing bacteria." NIPPON SUISAN GAKKAISHI. 56. 705 (1990)

T.Matsui：“通过施用产生河豚毒素的细菌来使养殖的河豚中毒。”

K.Yamamori: "Antagonism of the action tetrodotxin in a shore crab,Hemigrapsus sanguineus." Proceedings of the 10th world contress on animal,plant and microbial toxins,3ー8,Nov.1991,Singapore.

K. Yamamori：“岸蟹 Hemigrapsus sanguineus 河豚毒素作用的拮抗作用”，第十届世界动物、植物和微生物毒素大会论文集，1991 年 11 月 3-8 日，新加坡。