Origin and metabolism of tetrodotoxin in pufferfishes and newts

河豚毒素在河豚和蝾螈中的来源和代谢

基本信息

批准号：
02660203
负责人：
YAMAMORI Kunio
金额：
$ 1.34万
依托单位：
School of Fisheries Sciences, Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660203/
关键词：
pufferfish newt shore crab Tetrodotoxin origin metabolism detoxication 解毒

项目摘要

Supposing that the origin of tetrodotoxin(TTX)in pufferfishes and newts was exogenous, some studies on the origin and metabolism of TTX in these animals were conducted. 1. Toxicity of 27 species of marine invertebrates collected at Sanriku coast was examined, but no toxic animals were found. 2. To investigate the behavior of TTX in non-toxic cultured puffer fish, TTX was administered intravenously via an intravenous cannula. TTX concentration in the blood was progressively decreased in an hour after the administration, suggesting that TTX might be accumulated in certain organs. 3. A half of TTX administered intraperitoneally in non-toxic cultured pufferfish, was accumulated in liver. 4. Toxicity of a few species of invertebrates collected in a marsh inhabited by newts was examined, but no toxic animals were found. 5. There were remarkable individual and local variations of toxicity in newts. Comparison of the toxicity of newts collected from rice fields at different altitude suggested that the altitude has effects on the toxicity of newts. 6. Toxicity of newts changes with their growth. Adults are very toxic. Larvae are not so toxic. Toxicity of immature newts are not known. 7. The shore crab Hemigrapsus sanguineus was found to have anti-TTX substances with a high molecular weight in their body fluid. Preinjection of the body fluid into a mouse was effective to some extent in protecting from TTX challenge.

假设在河豚和NEWT中，四毒素（TTX）的起源是外源性的，那么对这些动物中TTX的起源和代谢的一些研究进行了研究。 1。检查了在Sanriku海岸收集的27种海洋无脊椎动物的毒性，但未发现有毒动物。 2。为了研究TTX在无毒培养的河豚鱼中的行为，TTX是通过静脉内插管静脉内给药的。在给药后一个小时内，血液中的TTX浓度逐渐降低，这表明TTX可能会在某些器官中累积。 3。在肝脏中积累了一半的TTX腹膜内腹膜内的TTX。 4。检查了在纽兹居住的沼泽中收集的几种无脊椎动物的毒性，但没有发现有毒动物。 5。在纽特的毒性中存在显着的个人和局部变化。比较从不同高度的稻田收集的NEW毒性的比较表明，高度对NEW的毒性有影响。 6。纽特的毒性随着其增长而变化。成人非常有毒。幼虫不是那么有毒。不成熟的纽约的毒性尚不清楚。 7。发现岸蟹半rapsusus sanguineus的体液中具有高分子量的抗TTX物质。将体液预注入小鼠可以在某种程度上有效保护TTX挑战。