Relationship between the skin and innert defense mechanisms in fish

鱼类皮肤与内防御机制的关系

• 批准号: 07456087
• 负责人: SUZUKI Yuzuru
• 金额: $ 4.8万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456087/
• 关键词: Fish; Skin; Defense mechanism; Lectin; Neutrophils; Opsonin; ウナギ; 白血球; 生体防御機構; 免疫; 食細胞

Opsonic activity of the skin mucus lectin against neutrophils was examined in the Japanese eel, Anguilla japonica, to elucidate if the defense mechanisms in the skin mucus has some meaning for the defense mechansms, e.g.leucocytes, when the infection occur via integument. Leuctin was extracted from the epithelial tissue and separated by gel filtration. This lectin agglutinated rabbit red blood cells (rRBC) with specificity to lactose. Eel neutrophils separated by Percoll density gradients were incubated with rRBC pretreated with the lectin, and phagocytic activity was estimated under microscopy. The lectin was found to enhance the phagocytosis of neutrophils, phagocytic index against lectin-bound rRBC was significantly higher than against normal rRBC with an increase in numbers of phagocytized rRBC.The activation of phagocytosis was inhibited by additive lectin inhibitor, i.e., lactose in the process of lectin treatment of rRBC.The skin mucus lectin in eel thus exhibited opsonic activity against eel neutrophils. Phagocytosis was influenced, e.g., enhanced or reduced accordingly by coexistent lymphocytes, showing modulational function of the lymphocytes. These findings show that the defense mechanisms of the skin surface have interrelationship with internal mechanisms.

本文研究了日本鳗鲡（Anguillajaponica）皮肤粘液凝集素对中性粒细胞的调理活性，以阐明当感染通过体壁发生时，皮肤粘液中的防御机制是否对防御机制（如白细胞）有某种意义。从上皮组织中提取亮氨酸并通过凝胶过滤分离。该凝集素凝集兔红细胞（rRBC），对乳糖具有特异性。Percoll密度梯度分离的鳗鱼中性粒细胞与凝集素预处理的rRBC孵育，并在显微镜下估计吞噬活性。结果表明，凝集素能增强中性粒细胞的吞噬功能，对凝集素结合的rRBC的吞噬指数显著高于对正常rRBC的吞噬指数，且吞噬rRBC的数量增加，而凝集素抑制剂则抑制了吞噬功能的激活。结果表明，鳗鱼皮肤粘液凝集素对鳗鱼中性粒细胞具有调理作用。吞噬作用受到影响，例如，共存的淋巴细胞相应地增强或降低，显示淋巴细胞的调节功能。这些发现表明，皮肤表面的防御机制与内部机制有相互关系。

项目成果

Kanlis et al.: "Immunoglobalin in occytes,fertilijed eggs and yolk saclarvol in red sea bream" Fish.Sci.61. 787-790 (1995)

Kanlis 等人：“红鲷中的卵细胞、受精卵和卵黄 saclarvol 中的免疫球蛋白”Fish.Sci.61。

Kanlis, G., Suzuki, Y., Tauchi, M., Numata, T., Shirojo, Y.and Takashima, F.: "Immunoglobulin in oocytes, fertilized eggs and yolk sac larvae in red sea bream." Fish.Sci.61. 789-790 (1995)

Kanlis, G.、Suzuki, Y.、Tauchi, M.、Numata, T.、Shirojo, Y.和 Takashima, F.：“红鲷卵母细胞、受精卵和卵黄囊幼虫中的免疫球蛋白。”

Suzuki,Y: "Ontogeny of the self defence mechanisms in Japanese eel" Prelim Rep.of Hakuho Maru Cruise KH-94-2(Leg3). 47-49 (1995)

Suzuki,Y：“日本鳗鱼自我防御机制的个体发育”，白凤丸游轮 KH-94-2（Leg3）的初步报告。

鈴木譲(分担): "水産動物の生体防御,pp.9-17「魚類の体表における防御反応」" 恒星社厚生閣,

铃木裕月（撰稿人）：“水生动物的生物防御，第9-17页“鱼体表面的防御反应””Kouseisha Koukaku，

Suzuki, y., Otaka, T., Sato, S., Hou, Y.Y.and Aida, K.: "Reproduction related immunoglobulin changes in rainbow trout." Fish Physiol.Biochem. (in press).

Suzuki, y.、Otaka, T.、Sato, S.、Hou, Y.Y. 和 Aida, K.：“虹鳟鱼繁殖相关的免疫球蛋白变化。”