Development of Lymphatic Tisues and Maternal Immunity in Fish Larvae.

鱼幼虫淋巴组织的发育和母体免疫力。

• 批准号: 03454082
• 负责人: SUZUKI Yuzuru
• 金额: $ 0.45万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454082/
• 关键词: Catfish; Larvae; Egg; Immunology; Maternal Immunity; Immunoglobulin; Lymphatic Tissue; Development; 魚類; 卵稚仔; 生体防御機構; 初期発生過程; モノクローナル抗体; 免疫; IgM

The early development of self-defense mechanisms walking catfish Clarias batrachus has been investigated from two different standpoints : development of lymphatic organs, and maternal immunity. In this fishes, kidney and thymus lymphopoiesis occur in the early stages of larval development, while spleen development is delayd. These observations suggest the low immuno-reactivity in fish larvae by their own immune systm.On the other hand the existence of egg IgM has been noted catfish. This was indicated by SDS-PAGE and western blotting technique using monoclonal antibody raised against H-chain of catfish IgM.Egg IgM is thought to be caused from maternal origin, and similar observations has been also made in carp Cyprinus carpio and red sea bream Pagras major by us. In particular, egg IgM from red sea bream vaccinated with Vibrio anguillarum exhibits anti-Vibrio activity, which demostrates the existence of maternal immunity. The egg IgM molecule is not necessarily the same as the plasma IgM molecules, as the H chain of egg IgM in catfish as well as carp has a lower molecular weight. Further investigations are needed to clarify the homogeneity of egg IgM to the plasma type IgM.

从淋巴器官发育和母体免疫两个不同的角度研究了鲇鱼自我防御机制的早期发展。在这种鱼中，肾脏和胸腺的淋巴系统在幼虫发育的早期阶段就开始形成，而脾脏的发育则延迟。这些观察结果表明，鱼苗自身免疫系统的免疫反应性较低。另一方面，卵细胞IgM的存在已引起鲶鱼的注意。采用针对鲶鱼IgM h链的单克隆抗体，通过SDS-PAGE和western blotting技术证实了这一点。卵IgM被认为是由母源引起的，我们在鲤鱼鲤鱼和红鲷鱼中也有类似的观察。特别是，接种过鳗弧菌的红鲷卵IgM具有抗弧菌活性，证明母体免疫的存在。卵IgM分子不一定与血浆IgM分子相同，因为鲶鱼和鲤鱼卵IgM的H链分子量较低。需要进一步的研究来阐明卵细胞IgM与血浆型IgM的同质性。

项目成果

会田、鈴木,植松: "生理「水産学シリーズ100号」日本水産学会編" 恒星社厚生閣,

会田、铃木、植松：《生理学‘水产科学系列第100号’，日本水产学会编》，Kouseisha Koukaku，

Y.Suzuki: "Granulocytes in the Process of Fish Inplammation" Ann.Rev.Fish.Diseases.

Y.Suzuki：“鱼类炎症过程中的粒细胞”Ann.Rev.Fish.Diseases。

Suzuki: "Blood,blood cell formation,and lymphatic tissue. in“Atlas of Fish Histology"ed.by Aida & Takachima" Gastav Fischer Verlag(in press),

铃木：“血液、血细胞形成和淋巴组织，见 Aida 和 Takachima 编辑的《鱼类组织学图集》”Gastav Fischer Verlag（正在出版），

Suzuki et al.: "Existence of low molecular weight immunoglobulin M in carp egg." Fisheries Science. (in press). (1994)

Suzuki 等人：“鲤鱼卵中存在低分子量免疫球蛋白 M。”

Y.Suzuki, M.Orito, K.Furukawa, and K.Aida: "Existence of low nolecular weight immunoglobulin M in Carp egg." Fisheries Science. (in press).

Y.Suzuki、M.Orito、K.Furukawa 和 K.Aida：“鲤鱼卵中存在低分子量免疫球蛋白 M”。