Sudies on the detoxication of lactic acid bacteria on mutagenicity of aflatoxin

乳酸菌解毒对黄曲霉毒素致突变性的研究

• 批准号: 07456120
• 负责人: HOSONO Akiyoshi
• 金额: $ 3.01万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456120/
• 关键词: aflatoxin; mutagenicity; lactic acid bacteria; detoxication; glia cell growth inhibition; fermented milk; Lactobacillus rhamnosus; 抗変異原性; ラット脳グリア細胞; アフラトキシンB_2

(1) Antimutagenic activities of milk cultured with five Lactobacillus strains on the mutagenicities of aflatoxins B_1, B_2 and G1 were investigated in vitro using Salmonella typhimurium TA 100 as the indicator bacteria. Cultured milk that was cultured with Lactobacillus acidophilus LA-2 strain had the highest inhibition (58.6 to 77.4%) again the mutagenicities of all the aflatoxins tested in S.typhimurium TA100 under the standard assay conditions with 8-9mix.(2) Effect of aflatoxins B_1, B_2, G_1, and G_2 on growth of glia cells was investigated by use of rats. Number of glia cells reached to ca. 1.5*10^5 cells/well after the incubation for 7 days in a well containing aflatoxin B_2, G_1or G_2, which was almost the same level to that observed in a control well. However, aflatoxin B_1 was observed to have strong inhibition to the growth of glia cells.(3) There was high percentage of binding in autoclave cells of Enterococcus faecalis FK-23 toward aflatoxins B_1, B_2, G_1, G_2.

(1)以鼠伤寒沙门氏菌TA-100为指示菌，研究了5株乳杆菌对黄曲霉毒素B_1、B_2和G_1的体外抗突变作用。在标准试验条件下，8- 9 mix，用嗜酸乳杆菌LA-2菌株培养的培养乳对鼠伤寒沙门氏菌TA 100中所有检测的黄曲霉毒素的致突变性具有最高的抑制率（58.6%至77.4%）。(2)本实验观察了黄曲霉毒素B_1、B_2、G_1、G_2对大鼠神经胶质细胞生长的影响。胶质细胞数达到约。在含有黄曲霉毒素B_2、G_1或G_2的孔中孵育7天后，细胞数为1.5*10^5个/孔，与对照孔中观察到的水平几乎相同。但黄曲霉毒素B_1对胶质细胞的生长有较强的抑制作用。(3)粪肠球菌FK-23高压灭菌细胞对黄曲霉毒素B_1、B_2、G_1、G_2有较高的结合率。

项目成果

Hosoda,M.,Hashimoto,H.,He,F.,Morita,H.and Hosono,A.: "Effect of administration of milk fermented with Lactobacillusacidophilus LA2 on fecal mutagenicity and microflora in humanintestine." J.Dairy Science. 79. 745-749 (1996)

Hosoda,M.、Hashimoto,H.、He,F.、Morita,H. 和 Hosono,A.：“使用嗜酸乳杆菌 LA2 发酵的牛奶对粪便致突变性和人体肠道微生物群的影响”。

Hosono,A.and Hisamatsu,S.: "Binding of amino acid pyrolysates and aflatoxins to autoclaved cells of Enterococcus faecalis." Biosci.Biotech.Biochem.59. 940-942 (1995)

Hosono,A. 和 Hisamatsu,S.：“氨基酸热解产物和黄曲霉毒素与粪肠球菌高压灭菌细胞的结合。”

Hosoda,M.,Hashimoto,H,,He,F.,Yamazaki,K.and Hosono,A.: "Inhibitory effect of milk cultured with Lactobacillus strainson the aflatoxin mutagenicity." Animal Science and Technology. (投稿審査中).

Hosoda, M.、Hashimoto, H,、He, F.、Yamazaki, K. 和 Hosono, A.：“用乳酸菌菌株培养的牛奶对黄曲霉毒素致突变性的抑制作用”（动物科学与技术）。

Hosoda, M., Hashimoto, H., He, F. Yamazaki, K. and Hosono, A.: "Inhibitory effect of milk cultured with Lactobacillus strainson the aflatoxin mutagenicity" Animal Science and Technology. 投稿審査中.

Hosoda, M.、Hashimoto, H.、He, F. Yamazaki, K. 和 Hosono, A.：“用乳酸菌菌株培养的牛奶对黄曲霉毒素致突变性的抑制作用”动物科学与技术提交的材料正在审查中。

Hosoda, M., Hashimoto, H., He, F., Morita, H.and Hosono, A.: "Effect of administration of milk fermented with Lactobacillus acidophilus LA2 on fecal mutagenicity and microflora in human intestine." J.Dairy Science. 79(5). 745-749 (1996)

Hosoda, M.、Hashimoto, H.、He, F.、Morita, H. 和 Hosono, A.：“使用嗜酸乳杆菌 LA2 发酵的牛奶对人肠道粪便致突变性和微生物群落的影响”。