Studies on Antimutagenic and Antitumoral Activities of Microbial Polysaccharides

微生物多糖抗突变和抗肿瘤活性研究

• 批准号: 01303011
• 负责人: HOSONO Akiyoshi
• 金额: $ 4.86万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01303011/
• 关键词: Microbial Polysaccharides; Antimutagenicity; Antitumor; Adjuband Effect; Fermented Milk; Superoxide Dismutase; Lactic Acid Bacteria; 微生物生産多糖類; ケフィ-ル; ダディヒ; アジェバンド効果; マイドジェル活性

Throughout 2 years research regarding to the subject described above, we obtained the following results.(1) Preparation of mutant strains with high producing abilities of polysaccharides :Mutant strains with high producing abilities of polysaccharides were prepared from the bacterial strains isolated from various kinds of fermented milk. Polysaccharides prepared from these mutant strains showed higher antitumoral activities compared with original strains.(2) Adjuvand effect of microbial polysaccharides :We confirmed the immunopotentiating activity on Mycobacterium tuberculosis, and detected little activity on Leuconostoc Paramesenteroides and Streptococcus lactis subsp. Diacetylactis.(3) Superoxide dismutase (SOD) activity :SOD activities of several strains of lactic acid bacteria was investigated. Among the strains tested St. salivarius subsp. Thermophilus demonstrated highest SOD production. General properties of partially purified SOD produced by this strain were also investigated.(4) Antimutagenic and antitumoral activities of polysaccharides prepared from the strains in traditional fermented milk products :Various strains of lactic acid bacteria were isolated from various types of traditional fermented milk products from Asian and East European countries. Peptideglycan fractions obtained from Leu. Dextranicum and Leu. Paramesenteroides demonstrated highest antimutagenic activity toward Trp-P1 and Trp-2. The kinetics of such amino acid pyrolyzates was found to be bandage to peptidoglycans. Polysaccharides prepared from Lactobacillus jugruti and Lb. Helveticus showed inhibition against the growth of Lewis lung carcinoma and Sarcoma-180. Chemical composition of polysaccharides prepared from these was also examined.

通过两年的研究，取得了以下研究结果：(1)高产多糖菌的筛选：从各种发酵乳中分离得到高产多糖菌，筛选出高产多糖菌。微生物多糖的佐剂和作用：证实了其对结核分枝杆菌的免疫增强活性，对副葡萄球菌和乳链球菌几乎没有活性。(3)超氧化物歧化酶活性：对几株乳酸菌的超氧化物歧化酶活性进行了研究。在所测试的菌株中，圣唾液亚种。嗜热菌具有最高的超氧化物歧化酶产量。对该菌株生产的部分纯化的超氧化物歧化酶的一般性质进行了研究。(4)传统发酵乳制品中多糖的抗突变和抗肿瘤活性：从亚洲和东欧国家不同类型的传统发酵乳制品中分离到不同菌株的乳酸菌。从亮氨酸中获得的多肽-葡聚糖组分。Dextraicum和Leu.副肠拟线虫对Trp-P1和Trp-2的抗诱变活性最高。这种氨基酸热解产物的动力学被发现是与肽多聚糖结合的。从乳杆菌和乳酸菌中提取的多糖。Helveticus对Lewis肺癌和S-180肉瘤的生长有抑制作用。并对其制备的多糖的化学组成进行了分析。

项目成果

Akiyoshi HOSONO,Masafumi SUZUKI and Hajime OTANI: "Superoxid Dismutage Activty in the Crude Cell Extract from Streptococcus salivarius subsp.thermophilus 3535." Anim.Sci.Technol.(Jpn.). 62. 39-44 (1991)

Akiyoshi HOSONO、Masafumi SUZUKI 和 Hajime OTANI：“唾液链球菌嗜热亚种 3535 粗细胞提取物中的超氧化物歧化活性。”

古川 徳: "微生物生産多糖投与による in vivoの抗腫瘍性発現の検討"

古川典宏：“通过施用微生物产生的多糖来检查体内抗肿瘤活性”

Hajime OTANI and Akiyoshi NOSONO: "The Chicken Egg Yolk Antibody Level as an Indicator to Assess Adjuvant Activity." Jpn. J. Dairy and Food Sci.

Hajime OTANI 和 Akiyoshi NOSONO：“鸡蛋黄抗体水平作为评估佐剂活性的指标。”

Hajime NAKAJIMA, Tetsuji HIROTA, Takahiro TOBA, Takatoshi ITOH and Susumu ADACHI: "Structure of the Extracellular Polysaccharide Viilian, from Slime-forming Lactococcus lactis subsp. cremoris SBT 0495." Carbohyd. Res.

Hajime NAKAJIMA、Tetsuji HIROTA、Takahiro TOBA、Takatoshi ITOH 和 Susumu ADACHI：“来自形成粘液的乳酸乳球菌乳脂亚种 SBT 0495 的细胞外多糖 Viilian 的结构。”

Hosono,A.,Tanabe,T.,Otani,H.: "Binding properties of lactic acid bacteria isolated from Kefir milk with mutagenic amino acid pyrolyzates." Milchwissenschaft. 45. 647-651 (1990)

Hosono,A.、Tanabe,T.、Otani,H.：“从开菲尔牛奶中分离出的乳酸菌与致突变氨基酸热解产物的结合特性。”