Studies on Antimutagenic and Antitumoral Activities of Microbial Polysaccharides

经过2年的研究关于上述主题，我们得到了以下结果。(1)高产多糖突变菌株的制备：从各种发酵乳中分离得到高产多糖突变菌株。从这些突变菌株制备的多糖与原菌株相比具有更高的抗肿瘤活性。(2)微生物多糖的辅助作用：证实其对结核分枝杆菌有免疫增强作用，对乳酸菌和乳酸菌亚群无免疫增强作用。Diacetylactis。(3)超氧化物歧化酶（SOD）活性：对几种乳酸菌的SOD活性进行了研究。在测试的菌株中，唾液链球菌亚种。嗜热菌的SOD产量最高。对该菌株部分纯化SOD的一般性质也进行了研究。(4)传统发酵乳制品中菌株制备的多糖的抗诱变和抗肿瘤活性：从亚洲和东欧国家不同类型的传统发酵乳制品中分离到多种乳酸菌菌株。从右旋亮氨酸和副亮氨酸中提取的肽聚糖对Trp-P1和Trp-2的抗诱变活性最高。这种氨基酸热解产物的动力学被发现与肽聚糖密切相关。从巨乳杆菌和Helveticus制备的多糖对Lewis肺癌和Sarcoma-180的生长有抑制作用。并对这些多糖的化学组成进行了研究。

Throughout 2 years research regarding to the subject described above, we obtained the following results.(1) Preparation of mutant strains with high producing abilities of polysaccharides :Mutant strains with high producing abilities of polysaccharides were prepared from the bacterial strains isolated from various kinds of fermented milk. Polysaccharides prepared from these mutant strains showed higher antitumoral activities compared with original strains.(2) Adjuvand effect of microbial polysaccharides :We confirmed the immunopotentiating activity on Mycobacterium tuberculosis, and detected little activity on Leuconostoc Paramesenteroides and Streptococcus lactis subsp. Diacetylactis.(3) Superoxide dismutase (SOD) activity :SOD activities of several strains of lactic acid bacteria was investigated. Among the strains tested St. salivarius subsp. Thermophilus demonstrated highest SOD production. General properties of partially purified SOD produced by this strain were also investigated.(4) Antimutagenic and antitumoral activities of polysaccharides prepared from the strains in traditional fermented milk products :Various strains of lactic acid bacteria were isolated from various types of traditional fermented milk products from Asian and East European countries. Peptideglycan fractions obtained from Leu. Dextranicum and Leu. Paramesenteroides demonstrated highest antimutagenic activity toward Trp-P1 and Trp-2. The kinetics of such amino acid pyrolyzates was found to be bandage to peptidoglycans. Polysaccharides prepared from Lactobacillus jugruti and Lb. Helveticus showed inhibition against the growth of Lewis lung carcinoma and Sarcoma-180. Chemical composition of polysaccharides prepared from these was also examined.