Studies on Antimutagenic and Antitumoral Activities of Microbial Polysaccharides

通过对上述课题的2年研究，我们获得了以下结果。(1)高产多糖突变株的制备：从各种发酵乳中分离菌株，制备高产多糖突变株。突变菌株制备的多糖具有较高的抗肿瘤活性。(2)微生物多糖的佐剂作用：我们证实了对结核分枝杆菌的免疫增强活性，并且检测到对副肠膜明串珠菌和乳酸链球菌亚种的几乎没有活性。二乙酰丙酮。(3)超氧化物歧化酶（SOD）活性：研究了几株乳酸菌的SOD活性。在供试菌株中，唾液圣杆菌亚种（St. salivarius subsp.）嗜热菌表现出最高的SOD生产。还研究了该菌株产生的部分纯化的SOD的一般性质。(4)从传统发酵乳制品中的菌株制备的多糖的抗突变和抗肿瘤活性：从来自亚洲和东欧国家的各种类型的传统发酵乳制品中分离出各种乳酸菌菌株。从Leu获得的肽聚糖级分。右旋糖酐和亮氨酸。Paramesenteroides对Trp-P1和Trp-2表现出最高的抗突变活性。这种氨基酸热解物的动力学被发现是绷带肽聚糖。从Jugruti乳杆菌和Lb. Helveticus对刘易斯肺癌和肉瘤180的生长有抑制作用。从这些制备的多糖的化学组成也进行了检查。

Throughout 2 years research regarding to the subject described above, we obtained the following results.(1) Preparation of mutant strains with high producing abilities of polysaccharides :Mutant strains with high producing abilities of polysaccharides were prepared from the bacterial strains isolated from various kinds of fermented milk. Polysaccharides prepared from these mutant strains showed higher antitumoral activities compared with original strains.(2) Adjuvand effect of microbial polysaccharides :We confirmed the immunopotentiating activity on Mycobacterium tuberculosis, and detected little activity on Leuconostoc Paramesenteroides and Streptococcus lactis subsp. Diacetylactis.(3) Superoxide dismutase (SOD) activity :SOD activities of several strains of lactic acid bacteria was investigated. Among the strains tested St. salivarius subsp. Thermophilus demonstrated highest SOD production. General properties of partially purified SOD produced by this strain were also investigated.(4) Antimutagenic and antitumoral activities of polysaccharides prepared from the strains in traditional fermented milk products :Various strains of lactic acid bacteria were isolated from various types of traditional fermented milk products from Asian and East European countries. Peptideglycan fractions obtained from Leu. Dextranicum and Leu. Paramesenteroides demonstrated highest antimutagenic activity toward Trp-P1 and Trp-2. The kinetics of such amino acid pyrolyzates was found to be bandage to peptidoglycans. Polysaccharides prepared from Lactobacillus jugruti and Lb. Helveticus showed inhibition against the growth of Lewis lung carcinoma and Sarcoma-180. Chemical composition of polysaccharides prepared from these was also examined.