Antimutagenic studies of Camembert cheese

卡芒贝尔奶酪的抗突变研究

• 批准号: 09660287
• 负责人: HOSONO Akiyoshi
• 金额: $ 1.98万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660287/
• 关键词: Camembert cheese; desmutagenicity; Trp-P-1; ripening; cheese from the marke; administration to rat; Ames strain; Trp-p1; Amesテスト; Penicillium roqueforti

Desmutagenic activity of Camembert cheese, containing the low molecular weight peptides produced during ripening, was studied in vitro against the mutagenicity of an amino acid pyrolysis product ; Trp-P-1, using the streptomycin-dependent strain (SD 510) of Salmonella typhimurium TA98. Also the effect of administration of freeze-dried Camembert cheese on the excretion of Trp-P-1 through urine and feces of rats were studied in vivo. The experimental cheese showed a gradual increase in water-soluble nitrogen during ripening. The amount of oligopeptides and smaller sized particles also increased with increase in ripening periods.The various types of cheese from the marke showed higher levels of water soluble N as percentage of total N, had high desmutagenicity than those of cheeses using only lactiq acid bacteria as starter. The desmutagenicity of the experimental Camembert cheese exhibited very high percentage, which increased during ripening period. Since preincubation of the reaction mixture in desmutagenic studies had no influence, the studies were done without preincubation. Effect of administration of freeze-dried Camembert cheese on the excretion of Tip-P-1 through urine and feces of rats were studied in vivo. Administration of the chow supplemented with 10% freeze-dried Camembert cheese showed no effect on urinary Tip-P-1 excretion compared to control. rats administrated with Trp-P-1 only, while that of 30% freeze-dried Camembert cheese showed decreased urinary Tip-P-1 excrection (P<0.05).

卡门贝尔奶酪，含有成熟过程中产生的低分子量肽的去诱变活性，进行了研究，在体外对氨基酸热解产物的致突变性;色氨酸-P-1，使用链霉素依赖性菌株（SD 510）的鼠伤寒沙门氏菌TA 98。还在体内研究了施用冻干Camembert奶酪对大鼠尿液和粪便中Trp-P-1排泄的影响。实验干酪在成熟过程中表现出水溶性氮的逐渐增加。不同类型的马克干酪的水溶性氮含量均高于单用乳酸菌发酵的干酪，且具有较高的抗诱变性。实验Camembert干酪的去致突变性表现出很高的百分比，在成熟期间增加。由于在去致突变研究中反应混合物的预孵育没有影响，因此在不进行预孵育的情况下进行研究。在体内研究了给予冻干Camembert奶酪对大鼠尿液和粪便中Tip-P-1排泄的影响。与对照组相比，补充有10%冻干卡门贝尔奶酪的食物的给药对尿Tip-P-1排泄没有影响。单用Trp-P-1组大鼠尿Tip-P-1排泄量明显低于30%冻干Camembert干酪组（P<0.05）。

项目成果

Yamada, M.et al.: "Antimutagenic activity of Camenbert cheese on the Trp-P1 induced mutagenicity to streptomycin-dependent strain SD510 of Salmonella typhimurium TA98." Int.Dairy J.7. 795-798 (1997)

Yamada, M.等人：“卡门贝尔奶酪对 Trp-P1 的抗诱变活性会诱导鼠伤寒沙门氏菌 TA98 的链霉素依赖性菌株 SD510 的诱变性。”

Yamada,M.et al.: "Antimutageic activity of Camenbert cheese on the Trp-P1 induced mutagenicity to streptomycin-dependent strain SD510 of Salmonella Typhimurium TA98." Int.Dairy J.7. 795-798 (1997)

Yamada,M.et al.：“卡门贝尔奶酪对 Trp-P1 的抗突变活性可诱导鼠伤寒沙门氏菌 TA98 的链霉素依赖性菌株 SD510 的致突变性。”

Yamada, M.et al.: "Desmutagenicity of commercial cheese against the Trp-P1 induced mutagenicity to streptomycin-dependent strain SD510 of Salmonella typhimurium TA98." Anim.Sci.Technol.359-364 (1998)

Yamada, M.等人：“商业奶酪对 Trp-P1 诱导的鼠伤寒沙门氏菌 TA98 链霉素依赖性菌株 SD510 的诱变性的去诱变性。”

Yamada,M.et al.: "Desmutagenicity of commercial cheese against the Trp-P1 induced mutagenicity to streptomycin-dependent strain SD510 of Salmonella typhimurium TA98." Anim.Sci.Technol.69. 359-364 (1998)

Yamada,M.等人：“商业奶酪对 Trp-P1 诱导的鼠伤寒沙门氏菌 TA98 链霉素依赖性菌株 SD510 的诱变性的去诱变性。”

Yamada, M. et al.: "Desmutagenicity of commer cial chees agalnst the Trip-p1 induced mutageniclty to streptomycin-dependent strain SD510 of Salmonella typhimurium TA98." Anim.Sci.Technol.69. 359-364 (1998)

Yamada, M. 等人：“商业奶酪针对 Trip-p1 诱导的鼠伤寒沙门氏菌 TA98 链霉素依赖性菌株 SD510 的诱变性的去诱变性。”