Histochemical and immunohistochemical studies on peptides and glucosaminoglycans in testes and accesory organs in bulls.

荷斯坦种公牛血清抑制素、FSH、LH和睾酮水平的变化：将515头荷斯坦种公牛分为0.2、0.5、1、2、3、4、5、6、7和8岁组。采集血样，收集后立即离心分离血清，并储存在-20 ℃下直至激素测定。血清卵泡刺激素（FSH）、黄体生成素（LH）水平。用放射免疫分析法（RIA）测定血清睾酮水平。1岁以前的青年公牛血浆中胆红素含量最高（0.2岁，2.46ng/ml ; 0.5岁，2.60ng/ml），随年龄增长而逐渐下降。与FSH相比，FSH在1岁以前较低（0.2岁，5.83ng/ml），随着年龄的增长而逐渐升高。血清LH水平随年龄增长有微弱的上升趋势，但不如FSH明显，而血浆睾酮水平在生后较低，随年龄增长逐渐上升。预 ...更多信息 结果表明，FSH、LH和睾酮水平与年龄有关。荷斯坦公犊从出生到青春期血清抑制素、FSH、LH和睾酮水平、睾丸抑制素和睾酮含量及抑制素免疫组织化学定位：新生犊牛睾丸抑制素含量最高（3.3ng/g湿重），随年龄增长而逐渐降低。8.6月龄公犊为0.52ng/g。甜菜碱含量与年龄呈正相关。血清睾酮含量从新生期到1.8月龄都很高（7.09ng/g湿重），随着年龄的增长逐渐下降。8.6月龄公犊为1.98ng/g。甜菜碱含量与年龄呈正相关。血清FSH和LH水平在1.6月龄时高于其他年龄组，但未发现明显的增龄或减龄变化。血清睾酮水平随日龄增加而升高，但与日龄无明显相关性，在新生至2.3月龄犊牛生精小管支持细胞中均检测到明显的阳性反应。此后，随着年龄的增长，它们的强度逐渐减弱，6个月以上的幼芽在化学上均未检测到。在6月龄以上小牛的任何支持细胞中也未检测到它们。少

Age-Related changes in the Serum Levels of Inhibin, FSH,LH and Testosterone in Holstein Bulls : Five-hundred-fifteen Holstain Bulls were divided into age-groups as 0.2,0.5,1,2,3,4,5,6,7 and 8years old. Bood samples were taken, and sera were separated immediately after collection by centrifugation and stored at -20ﾟCC until hormone assay. Serumlevel of inhibin, folicle stimurating hormone (FSH), luteinizing hormone (LH). and testosterone were assayd by radio immuno assay (RIA). The plasma levels of inhibin were the highest in the young bulls before 1year old (0.2year, 2.46ng/ml ; 0.5year, 2.60ng/ml) and gradually decreased with age. In contrast to inhibin, serum levels of FSH were the low in the young bulls before 1year old (0.2year old, 5.83ng/ml) and gradually increased with ages. The serum levels of LH were suggested a weak tendency to increase with age but not so clear as that of FSH.Plasma levels of testosterone were low at postnatal period and increased gradually with age. The pre … More sent results clatified age-related changes of inhibin, FSH.LH and testosterone. Seru, levels of inhibin were correlated negatively with FSH before the onset of the puberty.Serum Inhibin, FSH,LH and testosterone Levels, Testicular inhibin and Testosterone Contents and Immunohitochemical Localization of Inhibin in Holstein Bull Calves from Birth to Puberty : Teste sinhibin contents were the highest in neonatal calves (3.3ng/g wet weight) and decreased gradually with age. At 8.6month-old bull calves, they were 0.52ng/g. There was positive correlationship between the inhibin content and age. On the otherhand, testosterone contents did not show any age-related changes in this experimental period.Seruminhibin levels were very high from neonatal period to 1.8month-old calves (7.09ng/g wet weight) and decreased gradually with age. At 8.6month-old bull calves, they were 1.98ng/g. There was positive correlationship between the inhibin content and age. Serum FSH and LH levels were greater at 1.6month-old than other age groups, but clear increrasing or decreasing with age was not found in this sutdy. Serumtestosterone levels were increased with ages, but there was no clear correrationship with age.Positive reactions for inhibin localization were clearly detected in the Sertoli cells of the seminiferous tubules from neonatal to 2.3month-old calves. After that, their intensities were decreased with age, and they were not detected immunohistochemically over 6month-old claves. They were also not detected in any Sertoli cells of over 6month-old calves. Less