Histochemical and immunohistochemical studies on peptides and glucosaminoglycans in testes and accesory organs in bulls.

公牛睾丸和附属器官中肽和葡萄糖胺聚糖的组织化学和免疫组织化学研究。

• 批准号: 07456130
• 负责人: YAMADA Junzo
• 金额: $ 5.12万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456130/
• 关键词: Bull; Testes; Accesory sexual organs; Immunohistochemistry; Lectin; Inhibin; Peptides; RLA; 生殖器; 副生殖腺; レクチン; 複合糖質; ペプチド

Age-Related changes in the Serum Levels of Inhibin, FSH,LH and Testosterone in Holstein Bulls : Five-hundred-fifteen Holstain Bulls were divided into age-groups as 0.2,0.5,1,2,3,4,5,6,7 and 8years old. Bood samples were taken, and sera were separated immediately after collection by centrifugation and stored at -20ﾟCC until hormone assay. Serumlevel of inhibin, folicle stimurating hormone (FSH), luteinizing hormone (LH). and testosterone were assayd by radio immuno assay (RIA). The plasma levels of inhibin were the highest in the young bulls before 1year old (0.2year, 2.46ng/ml ; 0.5year, 2.60ng/ml) and gradually decreased with age. In contrast to inhibin, serum levels of FSH were the low in the young bulls before 1year old (0.2year old, 5.83ng/ml) and gradually increased with ages. The serum levels of LH were suggested a weak tendency to increase with age but not so clear as that of FSH.Plasma levels of testosterone were low at postnatal period and increased gradually with age. The pre … More sent results clatified age-related changes of inhibin, FSH.LH and testosterone. Seru, levels of inhibin were correlated negatively with FSH before the onset of the puberty.Serum Inhibin, FSH,LH and testosterone Levels, Testicular inhibin and Testosterone Contents and Immunohitochemical Localization of Inhibin in Holstein Bull Calves from Birth to Puberty : Teste sinhibin contents were the highest in neonatal calves (3.3ng/g wet weight) and decreased gradually with age. At 8.6month-old bull calves, they were 0.52ng/g. There was positive correlationship between the inhibin content and age. On the otherhand, testosterone contents did not show any age-related changes in this experimental period.Seruminhibin levels were very high from neonatal period to 1.8month-old calves (7.09ng/g wet weight) and decreased gradually with age. At 8.6month-old bull calves, they were 1.98ng/g. There was positive correlationship between the inhibin content and age. Serum FSH and LH levels were greater at 1.6month-old than other age groups, but clear increrasing or decreasing with age was not found in this sutdy. Serumtestosterone levels were increased with ages, but there was no clear correrationship with age.Positive reactions for inhibin localization were clearly detected in the Sertoli cells of the seminiferous tubules from neonatal to 2.3month-old calves. After that, their intensities were decreased with age, and they were not detected immunohistochemically over 6month-old claves. They were also not detected in any Sertoli cells of over 6month-old calves. Less

荷斯坦种公牛血清抑制素、卵泡刺激素、促黄体生成素和睾酮水平的增龄变化：将515头荷斯坦种公牛分为0.2、0.5、1、2、3、4、5、6、7和8岁年龄组。取BOD标本，离心后立即分离血清，保存于-20ﾟCC中，直至激素测定。血清抑制素、卵泡刺激素(FSH)、黄体生成素(LH)水平。用放射免疫分析法(RIA)测定血清睾酮水平。1岁以前的公牛血浆抑制素水平最高(0.2岁，2.46 ng/ml；0.5岁，2.60 ng/ml)，随着年龄的增长，抑制素水平逐渐下降。与抑制素相反，青年公牛血清FSH水平在1岁前最低(0.2岁，5.83 ng/ml)，并随着年龄的增长而逐渐升高。血清黄体生成素水平有随年龄增加的微弱趋势，但不如FSH水平明显。出生后血浆睾酮水平较低，随着年龄的增长逐渐升高。The Pre…更多的研究结果证实了抑制素、FSH.LH和睾酮的年龄相关性变化。血清抑制素水平与FSH在青春期前呈负相关。荷斯坦公牛从出生到青春期的血清抑制素、FSH、黄体生成素和睾酮水平、睾丸抑制素和睾酮含量以及抑制素的免疫组织化学定位：新生犊牛睾丸抑制素含量最高(3.3 ng/g湿重)，并随着年龄的增长逐渐下降。8.6月龄公犊的抑制素含量为0.52 ng/g，与日龄呈正相关。血清抑制素水平从新生期到1.8月龄都很高(7.09 ng/g湿重)，并随着年龄的增长而逐渐下降。8.6月龄公犊的抑制素含量为1.98 ng/g，与日龄呈正相关。1.6月龄血清FSH、LH值高于其他年龄组，但未见随年龄增长而明显升高或下降。血清睾酮水平随年龄增长而升高，但与年龄无明显相关性。从新生到2.3月龄，生精小管内的支持细胞均有明显的抑制素定位阳性反应。此后，随着年龄的增长，它们的强度逐渐降低，6月龄以上的锁骨免疫组织化学未检测到它们。在6月龄以上的小牛的支持细胞中也没有检测到它们。较少