Regulatory Mechanism for Oxalate Production by the Enzymes and Its Related System in Wood-rotting Fungi.

木材腐烂真菌酶及其相关系统产生草酸盐的调控机制。

• 批准号: 07456155
• 负责人: SHIMADA Mikio
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456155/
• 关键词: Oxalic acid; Glyoxylic acid; Flavohemoprotein; Glyoxylate dehydrogenase; Brown-rot fungi; White-rot fungi; Basidiomycetes; Tyomyces palustris; Oxalic acid; Glyoxylic acid; Biosynthesis; Glyoxylate oxidase; White-rot fungi; Brown-rot fungi; B

A role of oxalate producing enzyme systems in wood rotting fungi has not been elucidated. Most of brown rot fungi which attack wooden parts of houses have a strong activity to produce oxalic acid and they are tolerant to copper wood preservaties, including CCA (Copper, chrome and arsenate), because they detoxicate the copper with oxalate produced to form insoluble copper oxalate.As a result, following findings have been obtained.1.Oxalate which was known to inhibit the one elctron oxidation by lignin peroxidase was found also to inhibit the cleavage nonphenolic beta-O-4LCC model compound between lignin model moiety and glucose.2.Three enzymes, including oxalate producing enzyme (glyoxylate oxidase), oxalate decarboxylase, and formate dehydrogenase were isolated from a white-rot fungus Coriolus versicolor. Formate dehydrogenase was first cell-free extracted from wood rotting fungi by this investigation.3.We succeeded in isolating and purifying a new type of cytochrome c dependent glyoxylate dehydrogenase from copper tolerant brown rot fungus Tyromyces palustris. Spectral analysis of the enzyme showed that it belongs to a family of flavohemoproteins. FMN was found to be a prosthetic group. Thus, oxalate production was found to be catalyzed by the electron transport system with cytochrome c and may be controlled by inhibition of the electron transportation system.

草酸生产酶系统在木材腐烂真菌中的作用尚未阐明。褐腐菌大多具有很强的产草酸活性，对铜木材腐蚀物（包括CCA）具有一定的耐受性（铜、Chrome和砷酸盐），因为它们用产生的草酸盐使铜解毒，形成不溶性的草酸铜。结果，结果表明：1.草酸能抑制木质素过氧化物酶的单电子氧化作用，但也能抑制非酚类物质的裂解2.从白腐菌Coriolus versicolor中分离到3种酶，包括草酸生成酶（乙醛酸氧化酶）、草酸脱羧酶和甲酸脱氢酶。本研究首次从木腐菌中无细胞提取甲酸脱氢酶。3.从耐铜褐腐菌沼泽酪菌中分离纯化了一种新型的细胞色素c依赖的乙醛酸脱氢酶。光谱分析表明该酶属于黄血红素蛋白家族。FMN被发现是一个假体组。因此，草酸生产被发现是由细胞色素c的电子传递系统催化，并可能通过抑制电子传递系统控制。

项目成果

T.Tokimatsu et al.: "Degradation of non-phenolic β-ο-4 type lignin carbohydrate complex model compounds by lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium" Mokuzai Gakkaishi. 42. 173-179 (1996)

T. Tokimatsu 等人：“来自白腐真菌 Phanerochaete chrysosporium 的木质素过氧化物酶对非酚 β-ο-4 型木质素碳水化合物复合物模型化合物的降解”Mokuzai Gakkaishi 42. 173-179 (1996)。

M.Shimada et al.: "Possible biochemical roles of oxalic acid as a low moleular weight compound involved in browm-rot and white-rot wood decays." J.Biotechnology. 53. 103-113 (1997)

M.Shimada 等人：“草酸作为一种低分子量化合物，在褐腐病和白腐病木材腐烂过程中可能发挥的生化作用。”

Shimada,M.他4名: "Possible biochemical roles of oxalic acid as a low molecular weight compound involved in brown-rot and white-rot wood decays" Journal of Biotechnology. 54(in press). (1997)

Shimada, M. 和其他 4 人：“草酸作为一种低分子量化合物参与褐腐和白腐木材腐烂的可能生化作用”《生物技术杂志》54（出版中）。

T.Tokimatsu et al.: "Purification and characterization of cytochrome c dependent glyoxylate dehydrogenase as a new oxalate producing enzyme from Tyromyces palustris." Proceedings of the 7th International Conference on Pulp and Paper Industry, Vancouver, C

T.Tokimatsu 等人：“细胞色素 c 依赖性乙醛酸脱氢酶作为一种来自沼泽酪霉菌的新型草酸生产酶的纯化和表征。”

Mii,K.他2名: "Occurence of enzyme systems for production and decomposition of oxalate in a white-rot fungus Coriolus versicolor and some characteristics of glyoxylate oxidase" Wood Research. 83. 23-26 (1996)

Mii, K. 和其他 2 人：“白腐真菌云芝中草酸盐产生和分解的酶系统的发生以及乙醛酸氧化酶的一些特征”Wood Research 83. 23-26 (1996)。