权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

DEVELOPMETNT OF A NEW SCREENIG SYSTEM FOR WOOD PRSERVATIVES BY USE OF THE INHIBITION FOR WOOD ROTTING FUNGI

利用对木材腐烂真菌的抑制作用开发新的木材防腐剂筛选系统

基本信息

批准号：
13556024
负责人：
SHIMADA Mikio
金额：
$ 8.06万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13556024/
关键词：
Fomitopsis palustris copper-tolerant fungi isocitrate lyase oxalic acid wood-rotting fungi malate suynthase enzyme injibitors glyoxylate cycle 木材防腐菌木材防腐剤

项目摘要

The primary objective of this research project is to develop a new screening system for wood preservatives on the basis of biochemical analysis of the inhibitors blocking the key enzymes of the copper-tolerant wood rotting fungi. First we investigated metabolic mechanism for biosynthesis of oxalic acid which causes detoxification of copper preservatives, and finally, attempted to test many compounds if they inhibit potently the key enzyme isocitrate lyase in the in vitro inhibitor screening system.The following important findings obtained have been published in the international journals listed at the end of this abstract.1) The brown-rot fungus Fomitopsis palustris contains constitutively the glyoxylate cycle in contrast with the normal microorganisms grown on glucose and the cycle linked with the TCA cycle was also found to couple with the oxalic acid biosynthesis.2) Through these metabolic cycles coupling with the oxalate biosynthesis, the wood-rotting fungi seem to gain the energy for their growth and one of the key enzymes was found to be isocitrate lyase (ICL) which plays a pivotal role for biosynthesis of oxalic acid.3) Both ICL and malate synthase (MS) which are key enzymes of the glyoxylate cycle were found to distribute widely among the both white-rot and brown-rot fungi.4) We have successfully purified these key enzymes (ICL and MS) for the first time from the wood-rotting fungi and characterized for comparison with the enzymes from other sources.5) We also succeeded in making fruit bodies of the F.palustris in the liquid culture for the first time.

该研究项目的主要目标是基于对耐铜木材腐烂真菌关键酶抑制剂的生化分析，开发一种新的木材防腐剂筛选系统。首先，我们研究了导致铜防腐剂解毒的草酸生物合成的代谢机制，最后，尝试在体外抑制剂筛选系统中测试许多化合物是否有效抑制关键酶异柠檬酸裂合酶。获得的以下重要发现已发表在本摘要末尾列出的国际期刊上。1）褐腐真菌 Fomitopsis 与以葡萄糖为基础生长的正常微生物相比，沼泽菌本质上含有乙醛酸循环，并且还发现与 TCA 循环相关的循环与草酸生物合成耦合。2) 通过这些与草酸生物合成耦合的代谢循环，腐木真菌似乎获得了生长所需的能量，并且发现关键酶之一是异柠檬酸裂解酶（ICL）在草酸的生物合成中起着关键作用。3）乙醛酸循环的关键酶ICL和苹果酸合酶（MS）被发现广泛分布在白腐真菌和褐腐真菌中。4）我们首次从木腐真菌和褐腐真菌中成功纯化了这些关键酶（ICL和MS）并与其他来源的酶进行比较。5)我们还首次成功地在液体培养物中制备了沼泽沼泽菌的子实体。