Effects of dietary protein on NO production in the kidney and its evaluation found on food hygiene

膳食蛋白质对肾脏NO生成的影响及其食品卫生评价

基本信息

  • 批准号:
    07457094
  • 负责人:
  • 金额:
    $ 4.86万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1997
  • 项目状态:
    已结题

项目摘要

Sprague-Dawely rats Weighing approximately 200g were pair-fed isocaloric diets containing low amounts (6% casein) of protein, normal amounts (23% casein) of protein or high amounts (40% casein) of protein for 4 weeks. Each group of rats underwent sham operation or bilateral ureteral obstruction. Twenty four hours following operation, glomeruli were isolated by sieve techniques and total RNA was prepared according to the method of guanidinium thiocyanate. The expression of nitric oxide synthase (NOS)-1, NOS-2 and NOS-3mRNA was detected using the technique of polymerase chain reaction coupled to reverse transcription. Glomeruli from Shamoperated control (SOC) rats had enhanced expression of NOS-1 and NOS-3mRNA with increases in the amounts of dietary protein while they exhibited no expression of NOS-2mRNA under the same conditions. By contrast, the expression of NOS-2mRNA was angmented with increases in dietary protein intake in glomeruli from rats with bilateral ureteral obstruction (BUO). The expression of NOS-1 and NOS-3mRNA was reduced in glomeruli from rats with BUO when compared to SOC rats. Accordingly, high protein intake may exacerbate glomerular hemodynamics by decreasing NOS-1 and NOS-3mRNA.Also, it appears that increased NOS-2mRNA worsens glomerular hemodynamics via glomerular injury due to the biosynthesis of large amounts of the free radical, NO.Inversely, low protein intake may prevent the deterioration of glomerular hemodynamics by inhibiting a reduction in NOS-1 and NOS-3mRNA and a rise in NOS-2mRNA.
将体重约200 g的Sprague-Dawely大鼠成对喂食含有低量(6%酪蛋白)蛋白质、正常量(23%酪蛋白)蛋白质或高量(40%酪蛋白)蛋白质的等热量饮食4周。各组大鼠均行假手术或双侧输尿管梗阻。术后24 h,用筛网法分离肾小球,用硫氰酸胍法制备总RNA。采用聚合酶链反应-逆转录技术检测NOS-1、NOS-2、NOS-3 mRNA的表达。假手术对照组(SOC)大鼠肾小球NOS-1和NOS-3 mRNA表达随蛋白质摄入量的增加而增强,而NOS-2 mRNA表达无变化。而双侧输尿管梗阻(BUO)大鼠肾小球NOS-2 mRNA的表达随蛋白质摄入量的增加而增加。BUO大鼠肾小球NOS-1、NOS-3 mRNA表达较SOC大鼠减少。因此,高蛋白摄入可能通过降低NOS-1和NOS-3 mRNA的表达而使肾小球血流动力学恶化; NOS-2 mRNA的表达增加可能通过大量自由基NO的合成而损伤肾小球血流动力学;而低蛋白摄入可能通过抑制NOS-1和NOS-3 mRNA的表达减少和NOS-2 mRNA的表达增加而防止肾小球血流动力学恶化。

项目成果

期刊论文数量(20)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Yanagisawa: "Mechanism of the decreased eicosanoid production in vitro in respense toangiotenin II in glonemli of rats aid eitaterd weteral oletuition" Nephrology. 1. 191-197 (1995)
H.Yanagisawa:“大鼠肾小球血管紧张素 II 反应中类二十烷酸产生体外减少的机制有助于 eitaterd weteral oletuition”。
  • DOI:
  • 发表时间:
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    0
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  • 通讯作者:
柳沢裕之: "Eicosanoid production and levels fo newly characterized eicosanoid-forming enzymes in glomeruli and tubules of rat kidneys" Nephoolozy. 1. 31-38 (1995)
Hiroyuki Yanagisawa:“大鼠肾小球和肾小管中新鉴定的类二十烷酸形成酶的类二十烷酸产生和水平”Nephoolozy。 1. 31-38 (1995)
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    0
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  • 通讯作者:
H.Yanagisawa: "HgCl_2-induced acute renal failare and its pathophysiology" Jpm.J.Hygiene. 52. 1-6 (1998)
H.Yanagisawa:“HgCl_2 诱导的急性肾衰竭及其病理生理学”Jpm.J.Hygiene。
  • DOI:
  • 发表时间:
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    0
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N.Yanagisawa: "Eicosanoid production and levels of newly characterized eicosanoid-forming erzymes in different segment of rat nephrens" Nephrolozy. 1. 31-38 (1995)
N.Yanagisawa:“大鼠肾病不同节段中类二十烷酸的产生和新表征的类二十烷酸形成酶的水平”Nephrolozy。
  • DOI:
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  • 影响因子:
    0
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H.Yanagisawa: "Epcosanoid production and levels of newly characteriged eicosanoid-forminy enzymes in different seyments of rat neprons" Nephrology. 1. 31-38 (1995)
H.Yanagisawa:“大鼠肾病不同细胞中的类二十烷酸的产生和新特征的类二十烷酸形成酶的水平”肾脏病学。
  • DOI:
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  • 影响因子:
    0
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YANAGISAWA Hiroyuki其他文献

YANAGISAWA Hiroyuki的其他文献

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{{ truncateString('YANAGISAWA Hiroyuki', 18)}}的其他基金

Potential mechanisms responsible for aging promotion derived from the latent national disease, Zn-deficiency
潜在的民族疾病——缺锌,导致衰老的潜在机制
  • 批准号:
    21590715
  • 财政年份:
    2009
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Dietary protein restriction in rats with bilateral ureteral obstruction
双侧输尿管梗阻大鼠的饮食蛋白质限制
  • 批准号:
    05454217
  • 财政年份:
    1993
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Mechanism by which dietary protein moduletes renal hemodyr-amies
膳食蛋白质调节肾血红蛋白的机制
  • 批准号:
    03670262
  • 财政年份:
    1991
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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