PREDICTION OF CAUSATIVE AGENTS AGAINST OCCUPATIONAL HYPERSENSITIVITY PNEUMONITIS AND DETERMINATION OF THE THRESHOLD LIMIT VALUE

职业性过敏性肺炎病原体的预测及阈值的确定

• 批准号: 07457097
• 负责人: UEDA Atsushi
• 金额: $ 4.22万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457097/
• 关键词: occupational hypersensitivity pneumonitis; occupational allergy; fungi; agricultural medicine; industrial health; experiment of allergenesity; eggplant; alstromeria; 許容濃度; 反応予測

1)We conducted to establish an animal experimental model for predictive assessment of an allergenecity of hypersensitivity pneumonitis (HP) against materials born in occupational environment, especially fungi. Finally we adopted for development of HP in the lung tissue of c57Black/6 mice by repeated nasal administration of fungus antigen suspended in distillled water. The test fungus was Fusarium sp. sampled from the plastic greenhous planting eggplants and isolated by purified cultivation.The treated animals developed typical HP lesions in their lung tissue after total of 5 times exposure for 5 weeks.The result suggested that the trial model was useful and adequate to detect fungi sp. in the working places showing allergenesity to HP and also demonstrated firstly the allergenesity of HP of Fusarium sp.2)We made and proposed the flow chart for predictive ditection of allergenesity of HP and it's prevention among materials born in occupational environment, especially in agriculture, including the animal experimental model developed in the present research project.3)We clarified an immediate type allergenesity of an extract from eggplant component and a delayd type allergenesity of an extract of alstromeria both cultivated in plastic greenhouse and found allergic cases in workers of those cultivations.

1)建立了一种动物实验模型，用于预测职业性超敏性肺炎（hypersensitivity pneumonitis， HP）对职业性物质，尤其是真菌的致敏性。最后，我们采用反复经鼻给药蒸馏水悬浮真菌抗原的方法在c57Black/6小鼠肺组织中培养HP。试验真菌为从塑料大棚栽培茄子中提取的镰刀菌，经纯化培养分离得到。接受治疗的动物在5周内共暴露5次后，肺部组织出现典型的HP病变。结果表明，该试验模型可用于HP致敏工作场所真菌的检测，并首次证实了HP对镰刀菌的致敏性。2)我们制作并提出了HP致敏性预测及预防的流程图，包括本课题开发的动物实验模型。3)我们明确了在塑料温室栽培的茄子成分提取物的立即型过敏原和桤木提取物的延迟型过敏原，并在这些栽培的工人中发现了过敏病例。

项目成果

原田 幸一: "アルストロメリア接触皮膚炎のモルモットによる実験的研究" 日本農村医学会雑誌. 46(3). 629-629 (1997)

Koichi Harada：“豚鼠六出花接触性皮炎的实验研究”日本农村医学学会杂志 46(3) 629-629 (1997)。

原田 幸一: "アルストロメリア接触皮膚炎の実験的研究" 日本衛生学雑誌. 52(1). 419-419 (1997)

Koichi Harada：“六出花接触性皮炎的实验研究”日本卫生杂志 52(1) (1997)。

A.Ueda: "A model for prediction system of a hazard of hypersensitivity pneumonitis." J.Occup.Health. 40(suppl.). 713 (1998)

A.Ueda：“过敏性肺炎危害预测系统模型。”

原田 幸一: "フサリウム暴露によるマウスの過敏性肺炎に関する研究" 日本衛生学雑誌. 53(1). 227-227 (1998)

Koichi Harada：“镰刀菌暴露引起的小鼠过敏性肺炎的研究”，日本卫生杂志 53(1) (1998)。

A.Ueda: "Allergy among eggplant cultivators in the plastic greenhouse." Occup.Environ.Allergy. 4. 33 (1996)

A.Ueda：“塑料温室中的茄子种植者过敏。”