Cell-Molecular Biological Study on Detectin of Occult Metastatic Lesions in Lung Cancer

应用细胞角蛋白18单克隆抗体，应用免疫细胞化学方法对骨髓抽吸液中细胞角蛋白阳性细胞进行分析，以发现临床无血液远处转移的非小细胞肺癌隐匿性转移灶。在非小细胞癌中，34/64（53%）细胞角蛋白18阳性（I期32.3%，III期63.6%，IV期100%）。alpha_2、alpha_3、alpha_5、alpha_6、整合素亚基频繁表达。α _2整合素亚基阳性肺癌细胞角蛋白18阳性细胞检出率显著高于α _2亚基阴性组。在肺癌组织中，α _6beta_4整合素亚基与Catepsin B的表达呈极显著相关，整合素作为细胞粘附分子在肺癌隐匿性转移灶形成中起着非常重要的作用。特别是;在非小细胞癌中，Alpha_2beta_1整合素亚基可能有助于转移性转移从原发部位释放、迁移到血液和隐匿性转移形成。

In order to detect occult metastatic lesions in non-small cell lung cancer without clinically hematogenous distant metastasis, cytokeratin positive cell in bone marrow aspirates were analyzed by immunocytochemical method using monoclonal antibody to cytokeratin 18.Cytokeratin 18 positive cells were detected in 34/64 (53%) among non-small cell carcinoma (Stage I 32.3%, III 63.6%, IV 100%).The alpha_2, alpha_3, alpha_5, alpha_6, integrin subunits were frequently expressed. The detection rate of cytokeratin 18-positive cells of alpha_2 integrin subunitt positive lung cancer was significantly higher than that of the alpha_2 subunit negative group. A highly significant correlation was revealed between the alpha_6beta_4 integrin subunitt and the expression of Catepsin B in lung cancer,Integrins play very important roles as cellular adhesion molecules in occult metastatic lesion formation of lung cancer.Especially ; alpha_2beta_1 integrin subunit may contribute to metastatic release from a primary site, migration into bloodstream and occult matastatisis formation in non-small cell carcinoma.