Detection of lung adenocareinoma cells in peripheral blood by RT-PCR analysis for mRNA of surfactant protein

肺癌分期患者的5年生存率。1只有60-70%。肺癌的预后通常因乳房扩散而受损。为了检测外周血循环肺癌eelIs，我们对SP-A、SP-C和CC10的mRNA进行了RT-PCR分析。19例健康志愿者SP-A、SP-C、CC10 mrna表达均为阴性。99例非小细胞肺癌患者中有33例（33.3%）检测到SP-A mRNA。SP-CmRNA阳性率（14%）和CC10mRNA阳性率（8%）较低。SP-A mRNA在1期患者中的阳性表达率为26%，SPA mRNA的阳性率明显高于SP-C mRNA（8%）和CC-10mRNA（3%）。所有病例经根治性手术后SP-A mRNA阳性表达消失。RT-PCR检测SP-A mRNA对检测外周血中隐匿性转移性肺腺癌细胞有临床意义。

The 5 years survival of lung cancer patients with stage.1 is only 60-70%. The prognosis of lung cancer is usually impaired with melastalic dissemination. To detect circulating lung cancer eelIs in peripheral blood, we performed RT-PCR analysis for mRNA of SP-A, SP-C and CC10.The 19 samples from healthy volunteers were all negative for SP-A, SP-C, CC10 mRNA.expression. SP-A mRNA was detected in 33 patients (33.3%) with 99 nonsmall cell lung cancer. The positive rale of SP-CmRNA(14 %) and CC10mRNA(8 %) were low. The positivity of expression for SP-A mRNA in the patients with stage 1 was 26 %, and the positive rate of SPA mRNA was higher than SP-C mRNA (8 %) and CC-10mRNA(3 %) significantly. The positive samples of SP-A mRNA expression disappeared with all cases after a curative surgical operation.RT-PCR analysis for SP-A mRNA is clinically useful for the detection occult metastatic lung adenocarcinoma cells in periferal blood.