Possible reasons for immunological tolerance in rat liver transp lantation

在刘易斯（LEW）大鼠原位肝移植中，急性排斥反应发生在一过性，然后是移植物的自发接受。在前期的研究中，我们发现，在长期存活的受体WF大鼠中，致敏的细胞毒性T淋巴细胞前体（CTLp）被清除，并且进一步表明来自这些动物的LEW肝移植物没有同种异体刺激活性。在这项研究中，我们研究了移植物的免疫刺激活性的动力学变化和迁移供体细胞从移植物到受体脾脏。移植肝的同种异体刺激活性在移植后第1天和第2天观察到，并在移植后第3天消失。肝移植后12小时获得的受体WF大鼠的脾细胞显示出LEW的有效同种异体刺激能力，但在体内没有观察到这种反应与第3天获得的脾细胞。

In orthotopic liver transplantation from Lewis (LEW) into Wistar Furth (WF) rats, acute rejection response occurred transiently, followed by the spontaneous acceptance of the graft. In the previous study, we have found that primed cytotoxic T lymphocyte precursors (CTLp) were eliminated in long-term surviving recipient WF rats, and furthe rmore showed that LEW liver grafts from those animals had no allostimulatory activity. In this study, we investigated kinetic change in immunostimulatory activity of the graft and of the migrating donor cells from the graft into the recipient spleen. Allostimulatory activity of the liver graft was observed on days 1 and 2 and disappeared by day 3 after the trabsplantation. The spleen cells from recipient WF rats obtained 12 hours after liver transplantation showed potent allostimulatory capacity of LEW,but no such response was observed in vivo with the spleen cells obtained on day 3.