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Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome

产前香烟烟雾暴露：对后代肠道细菌微生物组的影响

基本信息

批准号：
10747173
负责人：
CYNTHIA CORBITT
金额：
$ 14.84万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10747173
关键词：
Acetates Age Age Months Air Attenuated Award Behavior Disorders Behavioral Biological Assay Biomedical Research Birth Weight Birth length Blood Glucose Brain Calorimetry Carbohydrates Cecum Characteristics Chemicals Child Coupled Data Development Dual-Energy X-Ray Absorptiometry Dyslipidemias Eating Eligibility Determination Euthanasia Exhibits Family Fasting Feces Female Fermentation Firmicutes First Generation College Students Funding Gene Expression Genes Glucose Glucose Intolerance Glucose tolerance test Goals Grant Growth Health Hepatic High Fat Diet Hormones Hour Immune system Immunologist Inflammation Inhalation Insulin Intervention Intestines Kentucky Latino Link Lipids Liver Low Birth Weight Infant Lupus Measures Mediating Metabolic Metabolic syndrome Modeling Mus Nutritional Organ Overweight Pancreas Parents Phenotype Plasma Pregnancy Production Proteobacteria Protocols documentation Puberty Publications Reporting Research Research Training Resistance Rural Same-sex Scanning Science Serum Signal Transduction Smoke Starch Structure Students Supplementation Testing Transplantation Underrepresented Minority Variant Volatile Fatty Acids Weaning Woman Work Writing absorption bacterial community bacteriome beta diversity career cigarette smoking dietary disadvantaged background dysbiosis economic disparity environmental tobacco smoke exposure exposure to cigarette smoke fetal tobacco exposure functional disability glucagon-like peptide 1 graduate school graduate student gut dysbiosis gut microbiome gut microbiota impaired glucose tolerance in utero insulin sensitivity interest male metabolic phenotype metabolomics microbial microbial composition microbiome microbiome alteration microbiome composition microbiota mouse model obesity risk offspring outcome disparities postnatal pregnant prenatal pup sex smoking during pregnancy symposium transcriptome sequencing transcriptomics

项目摘要

ABSTRACT This Research Supplement to Promote Diversity in Health-Related Research is to support a graduate student to perform research funded by R15 award 2R15ES028440-02A1, Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome. Mr. Isaiah Burciaga is eligible for this award on multiple fronts: he is an underrepresented minoritized (Afro-Latino) student in science from a rural, economically disadvantaged background who is a first-generation college student. He has demonstrated interest in a biomedical research career and has exhibited ability both academically and technically to fulfill that career goal. The studies proposed within the parent award use a well-developed murine model of developmental exposure to tobacco smoke. We have shown effects of prenatal cigarette smoke exposure (CSE) on birth weight, catch-up growth, and gut bacterial microbiome in mouse dams and their offspring, as well as effects on offspring phenotype. However, we do not know if the observed changes in microbiome and offspring phenotype are linked. We will measure the effect of cecal transfer of microbiomes or dietary resistant starch on those same measures in this model. Our central hypothesis, that CSE-induced gut microbiome alterations are linked to offspring phenotype, will be tested within 2 aims: Aim 1: use cecal transfer in weanlings to separate effects of CSE-induced microbiome from CSE itself Aim 2: use dietary resistant starch to increase short-chain fatty acids before vs after puberty If our central hypothesis is supported, additional studies will delve into gut-liver-brain axis consequences of intestinal dysbiosis, as well as investigate potential interventions. Mr. Burciaga’s research training will focus primarily on Aim 2 and will include participation in professional development activities through the UofL Graduate School, participation in scientific conferences, grant writing, and publication.

摘要这个研究补充，以促进健康相关研究的多样性是为了支持研究生进行由R15奖项2 R15 ES 028440 - 02 A1资助的研究，产前香烟烟雾暴露：影响关于后代肠道细菌微生物组的研究Isaiah Burciaga先生在多个方面都有资格获得这一奖项：他是一名来自农村、经济上处于不利地位的少数族裔（非洲裔-拉丁裔）学生在理科中的代表性不足背景是第一代大学生。他对生物医学研究很感兴趣职业生涯，并表现出能力，无论是学术和技术，以实现这一职业目标。研究在父母奖中提出使用发育暴露于烟草的成熟小鼠模型抽烟我们已经证明了产前香烟烟雾暴露（CSE）对出生体重、追赶性生长，和小鼠母体及其后代的肠道细菌微生物组，以及对后代表型的影响。然而，我们不知道观察到的微生物组和后代表型的变化是否存在联系。我们将测量微生物组或膳食抗性淀粉的盲肠转移对这些相同测量的影响，模型我们的中心假设是，CSE诱导的肠道微生物组改变与后代表型有关，将在2个目标内进行测试：目的1：在断奶仔猪中使用盲肠转移来分离CSE诱导的微生物组与CSE本身的影响目的2：在青春期前和青春期后使用抗性淀粉增加短链脂肪酸如果我们的中心假设得到支持，更多的研究将深入研究肠-肝-脑轴的后果，肠道生态失调，以及研究潜在的干预措施。Burciaga先生的研究培训将侧重于主要是目标2，并将包括通过UofL毕业生参与专业发展活动学校，参加科学会议，赠款写作和出版。